Molecular analysis of chicken interferon-alpha inducible
                        protein 6 gene and transcriptional regulation

Song, Ki-Duk; Park, Jeong-Woong; Ndimukaga, Marc; So, Jaerung; Kim, Su-Jung; Truong, Anh Duc; Tran, Ha Thi Thanh; Dang, Hoang Vu

doi:10.5187/jast.2022.e101

J Anim Sci Technol

2023

DOI: 10.5187/jast.2022.e101

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Molecular analysis of chicken interferon-alpha inducible protein 6 gene and transcriptional regulation

Ki-Duk Song¹,

Jeong-Woong Park²,

Marc Ndimukaga³

et al.

Abstract: Molecular analysis of chicken IFI6 gene and transcriptional regulation Running Title(English) chickne IFI6 gene and transcriptional expression

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Cited by 3 publications

References 47 publications

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Antiviral Effects of Avian Interferon-Stimulated Genes

He,

Zhang,

Zou

et al. 2024

Animals

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Interferons (IFNs) stimulate the expression of numerous IFN-stimulating genes via the Janus kinase-signal transducers and activators of the transcription (JAK-STAT) signaling pathway, which plays an important role in the host defense against viral infections. In mammals, including humans and mice, a substantial number of IFN-stimulated genes (ISGs) have been identified, and their molecular mechanisms have been elucidated. It is important to note that avian species are phylogenetically distant from mammals, resulting in distinct IFN-induced ISGs that may have different functions. At present, only a limited number of avian ISGs have been identified. In this review, we summarized the identified avian ISGs and their antiviral activities. As gene-editing technology is widely used in avian breeding, the identification of avian ISGs and the elucidation of their molecular mechanism may provide important support for the breeding of avians for disease resistance.

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Antiviral Effects of Avian Interferon-Stimulated Genes

He,

Zhang,

Zou

et al. 2024

Animals

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Regulation of Chicken FABP4 Transcription by Toll-Like Receptor 3 Activation in DF-1 Cells

So,

Kim,

Song

2023

Korean J. Poult. Sci.

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Long-chain fatty acids (LCFAs) are vital in cellular compartments, primarily regulating lipid metabolism. Fatty Acid-Binding Proteins (FABPs) facilitate LCFA transport, lipid synthesis, storage, and act as signaling molecules influencing various pathways, including inflammation. FABP4, in particular, is linked to vascular and cardio-related diseases, and it plays a role in macrophage-mediated inflammatory responses. Previous studies have identified FABP4 as not only a representative biomarker for lipogenesis but also as having correlations with immune responses. This study aims to investigate the regulation of the chicken FABP4 (chFABP4) gene by toll-like receptor 3 (TLR3) activation and determine the signaling pathways that are involved in chFABP4 transcriptional regulation. We analyzed the transcriptional regulation of chFABP4 in TLR3-stimulated DF-1 cells. The results showed that chFABP4 was up-regulated upon stimulation with polyinosinic-polycytidylic acid (PIC), a TLR3 ligand. Notably, chFABP4 transcription was independently regulated in the NF-κB signaling pathway. It was up-regulated in p38 inhibition, demonstrating that the p38 signaling pathway might suppress the transcription of chFABP4 within TLR3-activated DF-1 cells. In contrast, chFABP4 expression was down-regulated in JNK signaling pathway inhibition, suggesting the positive regulation of JNK signaling pathway for chFABP4 transcription in DF-1 cells in response to TLR3 activation, consistent with findings in macrophages. MEK pathway inhibition resulted in a similar regulation to NF-κB signaling. These results suggest that each MAPK contributes differentially to the transcriptional regulation of chFABP4 by in DF-1 cells in response to TLR3 activation.

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In vitro analysis of antiviral immune response against avian influenza virus in chicken tracheal epithelial cells

Heo,

Vu,

Kim

et al. 2024

Anim Biosci

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Objective: Avian influenza virus (AIV) infections first affect the respiratory tract of chickens. The epithelial cells activate the host immune system, which leads to the induction of immune-related genes and the production of antiviral molecules against external environmental pathogens. In this study, we used chicken tracheal epithelial cells (TECs) <i>in vitro</i> model to investigate the immune response of the chicken respiratory tract against avian respiratory virus infections.Methods: Eighteen-day-old embryonic chicken eggs were used to culture the primary chicken TECs. Reverse transcription-polymerase chain reaction (RT-PCR) and immunocytochemistry (ICC) analysis of epithelial cell-specific gene makers were performed to confirm the characteristics, morphology, and growth pattern of primary cultured chicken TECs. Moreover, to investigate the cellular immune response to AIV infection or polyinosinic-polycytidylic acid (poly [I:C]) treatment, the TECs were infected with the H5N1 virus or poly (I:C). Then, immune responses were validated by RT-qPCR and western blotting.Results: The TECs exhibited polygonal morphology and formed colony-type cell clusters. The RT-qPCR results showed that H5N1 infection induced a significant expression of antiviral genes in TECs. We found that TECs treated with poly (I:C) and exposed to AIV infection-mediated activation of signaling pathways, leading to the production of antiviral molecules (e.g., pro-inflammatory cytokines and chemokines), were damaged due to the loss of junction proteins. We observed the activation of the nuclear factor kappa B and mitogen-activated protein kinase (MAPK) pathways, which are involved in inflammatory response by modulating the release of pro-inflammatory cytokines and chemokines in TECs treated with poly (I:C) and pathway inhibitors. Furthermore, our findings indicated that poly (I:C) treatment compromises the epithelial cell barrier by affecting junction proteins in the cell membrane.Conclusion: Our study highlights the utility of <i>in vitro</i> TEC models for unraveling the mechanisms of viral infection and understanding host immune responses in the chicken respiratory tract.

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Molecular analysis of chicken interferon-alpha inducible protein 6 gene and transcriptional regulation

Abstract: Molecular analysis of chicken IFI6 gene and transcriptional regulation Running Title(English) chickne IFI6 gene and transcriptional expression

Cited by 3 publications

References 47 publications

Antiviral Effects of Avian Interferon-Stimulated Genes

Antiviral Effects of Avian Interferon-Stimulated Genes

Regulation of Chicken FABP4 Transcription by Toll-Like Receptor 3 Activation in DF-1 Cells

In vitro analysis of antiviral immune response against avian influenza virus in chicken tracheal epithelial cells

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