Molecular analysis of clonality of sporadic angiomyolipoma

Saxena, Anurag; Alport, Edward C.; Custead, Savita; Skinnider, Leo F.

doi:10.1002/(sici)1096-9896(199909)189:1<79::aid-path366>3.3.co;2-3

Cited by 3 publications

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“…Why does the fatty component reach such a huge mass? By molecular analysis of AML, Saxena et al reported that the muscle and the blood vessel components are monoclonal, whereas the adipose tissue is polyclonal and probably metaplastic or reactive 6 . It is postulated that some growth factors may excessively effect the adipose tissue in the case of huge AML.…”

Section: Discussionmentioning

confidence: 99%

A case of angiomyolipoma presenting as a huge retroperitoneal mass

et al. 2001

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A 60-year-old man was admitted to Hitachi General Hospital, Hitachi, Japan, with general fatigue and epigastric fullness. A large mass was palpated on whole abdomen and abdominal computed tomography scan showed a large lobulated fatty mass surrounding the right kidney, which indicated the existence of angiomyolipoma arising from the right kidney. The tumor was successfully resected through a thoracoabdominal incision. The total weight of the resected specimen was 3500 g, apparently the largest angiomyolipoma resected by operation in Japan.

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Section: Discussionmentioning

confidence: 99%

A case of angiomyolipoma presenting as a huge retroperitoneal mass

et al. 2001

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“…Saxena et al . recently reported a monoclonal pattern in smooth muscle cells and blood vessels of sporadic angiomyolipoma, while the adipose tissue revealed a polyclonal pattern4. Based on these findings the authors concluded that the polyclonal adipose tissue is probably metaplastic or reactive.…”

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confidence: 89%

“…Some of these considerations do not appear to have been addressed in the paper of Saxena et al 4,. especially those concerning tests for digestion completion with restriction endonuclease; controls regarding both tumour heterogeneity and their methylation patterns; PCR bias in the amplification of both alleles; tumour heterogeneity and patch size mosaicism; and the meaning of monoclonal and polyclonal patterns.…”

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confidence: 98%

Designing a molecular analysis of clonality in tumours

Díaz‐Cano

2000

J. Pathol.

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“…HUMARA is characterized by highly polymorphic trinucleotide-repeat (CAG) sequence proximal to methylation site with high incidence of heterozygosity [ 12 ], and the target gene amplicon is less than 300 bp. Those distinctiveness of the approach make it applicable to archival formalin-fixed tissues, which are often not amenable to analysis by a variety of alternative approaches [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Clonal analysis of palmar fibromatosis: a study whether palmar fibromatosis is a real tumor

Wang

Zhu

2006

J Transl Med

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BackgroundPalmar fibromatosis that arises in the palmar soft tissue is characterized by infiltrative growth with a tendency toward local recurrence but does not metastasize. This study investigated the clonality of this process in twelve female patients, each with a single lesion, by examining the pattern of X-chromosome inactivation.MethodsHematoxylin and eosin stained sections of formalin-fixed, paraffin-embedded tissues were microdissected by laser capture microdissection to obtain the proliferative spindle cells. Tumor cells were isolated from the sections of rectum adenocarcinoma, and used for positive control. The genomic DNAs was extracted with phenol-chloroform, digested with a methylation-sensitive restriction endonuclease HpaII, and amplified by polymerase chain reaction (PCR), using primers targeted to a highly polymorphic short tandem repeat (STR) of the human androgen receptor gene (HUMARA).ResultsAmong the twelve samples, three samples failed amplification, one sample showed homozygosity which was not suitable for further analysis, eight samples were successfully amplified, and showed a random X chromosome inactivation pattern, suggesting polyclonality of these lesions.ConclusionThe current findings suggest that palmar fibromatosis is a reactive proliferation rather than a clonal neoplasm.

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Molecular analysis of clonality of sporadic angiomyolipoma

Cited by 3 publications

References 0 publications

A case of angiomyolipoma presenting as a huge retroperitoneal mass

A case of angiomyolipoma presenting as a huge retroperitoneal mass

Designing a molecular analysis of clonality in tumours

Clonal analysis of palmar fibromatosis: a study whether palmar fibromatosis is a real tumor

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