Molecular Analysis of Drought Tolerance in Guava Based on In Vitro PEG Evaluation

Abou-Zaid, Eman A.A.; El-Sayed, El-Sayed N.; Elsayed, Mohamed; Youssef, Muhammad

doi:10.1007/s12042-016-9164-5

Cited by 8 publications

(3 citation statements)

References 26 publications

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“…Molecular analysis using simple and efficient tools such as ISSR and SCoT offers a good understanding and explaining for morphological and physiological observations. The two markers utilized in the current study (ISSR and/or SCoT) have been used previously in several studies, e.g., evaluation of genetic diversity (Youssef and Ibrahim, 2015;Khalil et al, 2020), abiotic stress screening (Abouzaid et al, 2016;El-Mahdy et al, 2021;El-Mahdy et al, 2022), mutation detection (Al-Qurainy, 2010) and assessment of heavy metals toxicity (Pisam et al, 2020). In the present study, ISSR and SCoT exposed their efficiency by confirming the observations at morphology and physiology levels.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Chromium harmful effects on in vitro banana performance

Youssef

Elsherbini

Morsy

2022

AJAS

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Abiotic stresses have a significant negative impact on plant growth and production. Among which, toxicity of heavy metals is considered one of the most factors affecting plant growth and agriculture on new reclaimed lands. In this study, the harmful effect of chromium was evaluated on in vitro banana plants of Grand Nain and Williams-Zeef cultivars based on morphological, physiological, and molecular assessments. Results showed that chromium significantly decreased all studied traits, including plant fresh weight, plant length, number of shoots per explant, and photosynthesis related pigments (chlorophyll and carotenoid content). The percentage of reduction due to chromium treatment ranged from 36.48 to 79.69% in shoot length of Grand Nain and chlorophyll-b in Williams-Zeef, respectively. Both banana cultivars were negatively affected by chromium treatment. However, Williams-Zeef showed higher reduction than that of Grand Nain in all studied traits. On the other hand, molecular analysis was performed to detect any variation between chromium-treated and untreated plants using inter simple sequence repeats (ISSR) and start codon targeted (SCoT) polymorphism. Results of molecular analysis confirmed the morphophysiological findings, by detection some polymorphic bands due to chromium treatment. In this regard, a total of six polymorphic bands were detected in the two banana cultivars, discriminating treated and control plants. In agreement with morphophysiological results, Williams-Zeef showed more polymorphism (five bands) due to chromium treatment than Grand Nain (one band). The screening protocol used in this study was efficient and helpful and could be used in successive studies to evaluate other toxicants and with other plant species as well.

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Section: Discussionmentioning

confidence: 99%

Evaluation of Chromium harmful effects on in vitro banana performance

Youssef

Elsherbini

Morsy

2022

AJAS

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“…Screening of drought tolerant and susceptible landraces of guava using SRAP and ISSR markers have shown to be effective and promising in analyzing germplasms tolerant to drought [ 190 ]. On the other hand, 40 potential microRNAs related to salinity stress and their target transcripts were characterized in guava [ 103 ].…”

Section: Abiotic Stressmentioning

confidence: 99%

Genomic Approaches for Improvement of Tropical Fruits: Fruit Quality, Shelf Life and Nutrient Content

Mathiazhagan

Chidambara

Laxman

et al. 2021

Genes

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The breeding of tropical fruit trees for improving fruit traits is complicated, due to the long juvenile phase, generation cycle, parthenocarpy, polyploidy, polyembryony, heterozygosity and biotic and abiotic factors, as well as a lack of good genomic resources. Many molecular techniques have recently evolved to assist and hasten conventional breeding efforts. Molecular markers linked to fruit development and fruit quality traits such as fruit shape, size, texture, aroma, peel and pulp colour were identified in tropical fruit crops, facilitating Marker-assisted breeding (MAB). An increase in the availability of genome sequences of tropical fruits further aided in the discovery of SNP variants/Indels, QTLs and genes that can ascertain the genetic determinants of fruit characters. Through multi-omics approaches such as genomics, transcriptomics, metabolomics and proteomics, the identification and quantification of transcripts, including non-coding RNAs, involved in sugar metabolism, fruit development and ripening, shelf life, and the biotic and abiotic stress that impacts fruit quality were made possible. Utilizing genomic assisted breeding methods such as genome wide association (GWAS), genomic selection (GS) and genetic modifications using CRISPR/Cas9 and transgenics has paved the way to studying gene function and developing cultivars with desirable fruit traits by overcoming long breeding cycles. Such comprehensive multi-omics approaches related to fruit characters in tropical fruits and their applications in breeding strategies and crop improvement are reviewed, discussed and presented here.

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“…The genetic potential of drought resistance using PEG solution with an osmotic potential of -1.03 MPa was evaluated in 20 different sorghum seedlings (Bibi et al, 2012). Abouzaid et al (2016) tested five concentrations of PEG in guava, among which the optimum drought concentration for screening was determined as 8%.…”

Section: Introductionmentioning

confidence: 99%

Drought resistance indicators screening and drought resistance evaluation of Salvia miltiorrhiza Bunge germplasm resources at seedling stage

Zhang,

Meng

et al. 2024

Chil. j. agric. res.

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Drought is a significant abiotic constraint in Salvia miltiorrhiza production, particularly during seedling cultivation. Therefore, researching drought resistance is crucial. This study aimed to investigate the effects of drought stress on the agronomic and physiological characteristics of S. miltiorrhiza at the seedling stage, evaluate drought resistance of various germplasms, and identify drought resistance markers. To simulate drought stress, five different concentrations of polyethylene glycol (PEG) (0%, 5%, 10%, 15%, and 20%) were applied to four S. miltiorrhiza germplasms at the seedling stage. Agronomic traits, root morphology, and physiological and biochemical indicators were measured. The data were then analyzed using the membership function method, correlation analysis, and stepwise regression analysis. The results revealed that as the PEG concentration increased, the aboveground agronomic traits and underground root morphology of the four S. miltiorrhiza germplasms showed a decline. The activities of catalase and peroxidase initially increased and then decreased, while the contents of soluble sugar (SS), soluble protein, and proline increased compared to the control. Based on the membership function values of 16 morphological and physiological indicators, the drought resistance order of the four S. miltiorrhiza germplasms was as follows: Yudanshen VD > Yudanshen VA > Yudanshen VB > Yudanshen VC. Additionally, an optimal regression equation (D = 0.887X1 -0.003X2 + 0.142X3) was developed through stepwise regression analysis, indicating that X1, X2, and X3 represent the maximum root length, root surface area, and SS content, respectively. Consequently, this study presents a comprehensive and reliable method for evaluating drought resistance in S. miltiorrhiza germplasm resources.

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Molecular Analysis of Drought Tolerance in Guava Based on In Vitro PEG Evaluation

Cited by 8 publications

References 26 publications

Evaluation of Chromium harmful effects on in vitro banana performance

Evaluation of Chromium harmful effects on in vitro banana performance

Genomic Approaches for Improvement of Tropical Fruits: Fruit Quality, Shelf Life and Nutrient Content

Drought resistance indicators screening and drought resistance evaluation of Salvia miltiorrhiza Bunge germplasm resources at seedling stage

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