Molecular analysis of endo-β-mannanase genes upon seed imbibition suggest a cross-talk between radicle and micropylar endosperm during germination of<i>Arabidopsis thaliana</i>

Iglesias‐Fernández, Raquel; Rodríguez-Gacio, María del Carmen; Barrero-Sicilia, Cristina; Carbonero, Pilar; Matilla, Ángel J.

doi:10.4161/psb.6.1.14126

Cited by 12 publications

(16 citation statements)

References 21 publications

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“…Mannan‐type oligosaccharides are thought to be produced by endo‐1,4‐β‐mannanase (E.C.3.2.1.78) (MAN) that catalyzes the cleavage of β(1–4) bonds in the backbones of mannan polymers (Shallom and Shoham, ). Plant MAN s are reported to be involved in the seed germination and post‐germination process by promoting hydrolysis of mannan‐rich endosperm CWs (Bewley et al ., ; Nonogaki et al ., ; Gong and Bewley, ; Iglesias‐Fernandez et al ., ,b; Rodriguez‐Gacio Mdel et al ., ). In tomato, LeMAN1 is expressed in the endosperm of germinated seeds and plays a role in hydrolyzing stored polysaccharides to provide germinating seeds with its energy and carbon source needs (Bewley et al ., ; Nonogaki et al ., ).…”

Section: Introductionmentioning

confidence: 99%

“…In tomato, LeMAN1 is expressed in the endosperm of germinated seeds and plays a role in hydrolyzing stored polysaccharides to provide germinating seeds with its energy and carbon source needs (Bewley et al ., ; Nonogaki et al ., ). Tomato LeMAN2 (Nonogaki et al ., ) and Arabidopsis AtMAN5 , AtMAN6 and AtMAN7 (Iglesias‐Fernandez et al ., ,b) are involved in the hydrolysis of mannan‐rich CWs to allow for radicle emergence and the completion of germination. MAN activity was also detected in CW softening associated with fruit ripening (Bewley et al ., ; Schroder et al ., ).…”

Section: Introductionmentioning

confidence: 99%

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Populus endo‐beta‐mannanase PtrMAN6 plays a role in coordinating cell wall remodeling with suppression of secondary wall thickening through generation of oligosaccharide signals

et al. 2013

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SUMMARYEndo-1,4-b-mannanase is known to able to hydrolyze mannan-type polysaccharides in cell wall remodeling, but its function in regulating wall thickening has been little studied. Here we show that a Populus endo-1,4-b-mannanase gene, named PtrMAN6, suppresses cell wall thickening during xylem differentiation. PtrMAN6 is expressed specifically in xylem tissue and its encoded protein localizes to developing vessel cells. Overexpression of PtrMAN6 enhanced wall loosening as well as suppressed secondary wall thickening, whilst knockdown of its expression promoted secondary wall thickening. Transcriptional analysis revealed that PtrMAN6 overexpression downregulated the transcriptional program of secondary cell wall thickening, whilst PtrMAN6 knockdown upregulated transcriptional activities toward secondary wall formation. Activity of PtrMAN6 hydrolysis resulted in the generation of oligosaccharide compounds from cell wall polysaccharides. Application of the oligosaccharides resulted in cellular and transcriptional changes that were similar to those found in PtrMAN6 overexpressed transgenic plants. Overall, our results demonstrated that PtrMAN6 plays a role in hydrolysis of mannan-type wall polysaccharides to produce oligosaccharides that may serve as signaling molecules to suppress cell wall thickening during wood xylem cell differentiation.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Populus endo‐beta‐mannanase PtrMAN6 plays a role in coordinating cell wall remodeling with suppression of secondary wall thickening through generation of oligosaccharide signals

et al. 2013

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“…By contrast, an increased GA-ABA ratio is the most important factor for the integration of the environmental and intrinsic signals for the occurrence of germination. This enhanced GA-ABA balance raises the expression of hydrolase genes such as those encoding cell wall-remodeling enzymes, involved in the weakening of the embryo-surrounding tissues during germination sensu stricto, and of reserve mobilization enzymes post germination (Gubler et al, 1995(Gubler et al, , 1999Mena et al, 2002;Iglesias-Fernández et al, 2011a, 2011cGonzález-Calle et al, 2015).…”

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confidence: 99%

A Developmental Switch of Gene Expression in the Barley Seed Mediated by HvVP1 (Viviparous-1) and HvGAMYB Interactions

Abraham¹,

Iglesias‐Fernández²,

Martínez³

et al. 2016

Plant Physiol.

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The accumulation of storage compounds in the starchy endosperm of developing cereal seeds is highly regulated at the transcriptional level. These compounds, mainly starch and proteins, are hydrolyzed upon germination to allow seedling growth. The transcription factor HvGAMYB is a master activator both in the maturation phase of seed development and upon germination, acting in combination with other transcription factors. However, the precise mechanism controlling the switch from maturation to germination programs remains unclear. We report here the identification and molecular characterization of Hordeum vulgare VIVIPAROUS1 (HvVP1), orthologous to ABA-INSENSITIVE3 from Arabidopsis thaliana. HvVP1 transcripts accumulate in the endosperm and the embryo of developing seeds at early stages and in the embryo and aleurone of germinating seeds up to 24 h of imbibition. In transient expression assays, HvVP1 controls the activation of Hor2 and Amy6.4 promoters exerted by HvGAMYB. HvVP1 interacts with HvGAMYB in Saccharomyces cerevisiae and in the plant nuclei, hindering its interaction with other transcription factors involved in seed gene expression programs, like BPBF. Similarly, this interaction leads to a decrease in the DNA binding of HvGAMYB and the Barley Prolamine-Box binding Factor (BPBF) to their target sequences. Our results indicate that the HvVP1 expression pattern controls the full Hor2 expression activated by GAMYB and BPBF in the developing endosperm and the Amy6.4 activation in postgerminative reserve mobilization mediated by GAMYB. All these data demonstrate the participation of HvVP1 in antagonistic gene expression programs and support its central role as a gene expression switch during seed maturation and germination.

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“…At these intermediate ranges of moisture level, an increase was observed in the germination values for both drying methods (Table 2). Endo-β-mannanase activity is associated with the mobilization of endosperm reserves, making these reserves available for seedling growth and development (Iglesias-Fernandez et al, 2011). The cells that cover the radicle exhibit mechanical resistance to cellular elongation during germination.…”

Section: Discussionmentioning

confidence: 99%

“…In contrast, the cell walls of embryonic cells are not rich in mannans; therefore, they are not digested by endo-β-mannanase. In this way, cellular elongation potential overcomes the physical resistance of the endosperm during germination (Iglesias-Fernandez et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Gene expression of coffee seed oxidation and germination processes during drying

Santos¹,

Pinho²,

Rosa³

2013

Genet. Mol. Res.

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ABSTRACT. Coffee (Coffea arabica L.) seeds are sensitive to desiccation and oxidative stress during drying processes. We investigated the effect of drying and moisture levels on germinationrelated gene expressions associated with enzymatic systems that prevent oxidative stress in coffee seeds. Coffee seeds collected at physiological maturity were subjected to slow and quick drying to 40, 30, 20, and 12% moisture levels (wet basis), and as the control, seeds without drying were used. The seeds' physiological quality was calculated as percentage of normal seedlings at 15 and 30 days, normal vigorous seedlings at 30 days, and cotyledonary leaves at 45 days. The isoenzymes esterase, catalase (CAT), peroxidase (POX), and endo-β-mannanase expressions were electrophoretically analyzed. CAT and POX expressions were analyzed using RT-qPCR with specific primers constructed from the target gene sequences from the Brazilian Coffee Genome Database. Slow drying showed better physiological quality for seeds at 40 and 12% moisture levels, while quick drying was the most effective for seeds with 20% moisture. Sensitivity to water loss was confirmed by quick drying and activation of enzymes. CAT and POX transcriptions reduced during drying. RT-qPCR revealed a 6969 ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 12 (4): 6968-6982 (2013) Gene expression during coffee seed drying process complex gene-expression pattern during the oxidative process, with high gene expression in wet seeds.

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Molecular analysis of endo-β-mannanase genes upon seed imbibition suggest a cross-talk between radicle and micropylar endosperm during germination ofArabidopsis thaliana

Cited by 12 publications

References 21 publications

Populus endo‐beta‐mannanase PtrMAN6 plays a role in coordinating cell wall remodeling with suppression of secondary wall thickening through generation of oligosaccharide signals

Populus endo‐beta‐mannanase PtrMAN6 plays a role in coordinating cell wall remodeling with suppression of secondary wall thickening through generation of oligosaccharide signals

A Developmental Switch of Gene Expression in the Barley Seed Mediated by HvVP1 (Viviparous-1) and HvGAMYB Interactions

Gene expression of coffee seed oxidation and germination processes during drying

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