Molecular analysis of group A rotaviruses detected in adults and adolescents with severe acute gastroenteritis in Italy in 2012

Ianiro, Giovanni; Delogu, Roberto; Bonomo, Paolo; Fiore, Lucia; Ruggeri, Franco Maria

doi:10.1002/jmv.23871

Cited by 14 publications

(6 citation statements)

References 32 publications

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“…Although the number of different G–P combinations was very high, approximately 83.7% of rotavirus strains circulating in our study groups were defined in six common P-G combinations such as G9P[8] genotype (40.5%), followed by G1P[8] (21.6%), G2P[8] (9.3%), G2P[4] (6.5%), G3P[8] (3.5%), and G4P[8] (3.4%). These results are in agreement with the findings indicating that the five common G and P combinations (G1P[8], G2P[4], G3P[8], G4P[8], and G9P[8]) account for approximately 90% of all human rotavirus strains [12] , [28] , [38] , [40] . We observed statistically significant differences in the prevalence of these common genotypes between different regions.…”

Section: Discussionsupporting

confidence: 92%

Prevalence of Rotavirus Genotypes in Children Younger than 5 Years of Age before the Introduction of a Universal Rotavirus Vaccination Program: Report of Rotavirus Surveillance in Turkey

Durmaz

Kalaycioglu²,

Acar³

et al. 2014

PLoS ONE

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BackgroundGroup A rotaviruses are the most common causative agent of acute gastroenteritis among children less than 5 years of age throughout the world. This sentinel surveillance study was aimed to obtain baseline data on the rotavirus G and P genotypes across Turkey before the introduction of a universal rotavirus vaccination program.MethodsRotavirus antigen-positive samples were collected from 2102 children less than 5 years of age who attended hospitals participating in the Turkish Rotavirus Surveillance Network. Rotavirus antigen was detected in the laboratories of participating hospitals by commercial serological tests such as latex agglutination, immunochromatographic test or enzyme immunoassay. Rotavirus G and P genotypes were determined by reverse transcription polymerase chain reaction (RT-PCR) using consensus primers detecting the VP7 and VP4 genes, followed by semi-nested type-specific multiplex PCR.ResultsRT-PCR found rotavirus RNA in 1644 (78.2%) of the samples tested. The highest rate of rotavirus positivity (38.7%) was observed among children in the 13 to 24 month age group, followed by children in the age group of 25 to 36 months (28.3%). A total of eight different G types, six different P types, and 42 different G–P combinations were obtained. Four common G types (G1, G2, G3, and G9) and two common P types (P[8] and P[4]) accounted for 95.1% and 98.8% of the strains, respectively. G9P[8] was the most common G/P combination found in 40.5% of the strains followed by G1P[8] (21.6%), G2P[8] (9.3%), G2P[4] (6.5%), G3P[8] (3.5%), and finally, G4P[8] (3.4%). These six common genotypes included 83.7% of the strains tested in this study. The rate of uncommon genotypes was 14%.ConclusionThe majority of the strains analyzed belonged to the G1–G4 and G9 genotypes, suggesting high coverage of current rotavirus vaccines. This study also demonstrates a dramatic increase in G9 genotype across the country.

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Section: Discussionsupporting

confidence: 92%

Prevalence of Rotavirus Genotypes in Children Younger than 5 Years of Age before the Introduction of a Universal Rotavirus Vaccination Program: Report of Rotavirus Surveillance in Turkey

Durmaz

Kalaycioglu²,

Acar³

et al. 2014

PLoS ONE

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“…The aim of this study was to describe the epidemiologic feature of RVA and the RVA-related disease burden among acute gastroenteritis patients under 5 years of age in Kunming, China, in 2015. The prevalence of RVA (28.5%) in this study was approximately equal to that in a 5-year surveillance study conducted in China (29.7%) [8], but the prevalence of RVA in this study was higher than those in other studies conducted in developed countries such Japan, Belgium, and Italy (11.9, 6.4, and 2.6%, respectively), where the RVA vaccine has been introduced in national immunization programs [11][12][13]. The prevalence of RVA is still as high as 34.0% in diarrhea Common types (total) 279 (79.9) G1P (8) 24 (6.9) G3P (8) 52 (14.9) G9P (8) 203 (58.2) Uncommon types (total) 30 (8.6) G1P (4) 3 (0.9) G2P (6) 2 (0.6) G2P (8) 3 (0.9) G3P (4) 4 (1.1) G3P (9) 2 (0.6) G4P (4) 1 (0.3) G4P (6) 2 (0.6) G4P (8) 3 (0.9) G8P (9) 4 (1.1) G9P (4) 4 (1.1) G9P (6) 2 (0.6) Mixed types (total) 24 (6.9) G1G2P (4) 3 (0.9) G1G9P (8) 2 (0.6) G2P(4)P (8) 1 (0.3) G3G1P (8) 18 ( cases in low-income and middle-income countries, where RVA vaccination is not obtained.…”

Section: Discussioncontrasting

confidence: 70%

Epidemiology and Genetic Diversity of Rotavirus in Kunming, China, in 2015

Kang

Cai

2018

Intervirology

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Group A rotavirus (RVA) is a serious public problem in China, with a prevalence of 29.7% in diarrhea cases under 5 years of age. A total of 1,224 fecal specimens were collected in 2015 from children under 5 years of age with acute gastroenteritis in Kunming, China, for detection and characterization of rotavirus. The prevalence of RVA was 28.5%. The frequency of RVA detection was greatest (52%) among children aged 7–12 months. The following strains were the most common types: G9P(8) (58.2%), G3P(8) (14.9%), and G1P(8) (6.9%). The following strains were uncommon types: G3P(4), G8P(9), and G9P(4) (1.1%); G1P(4), G2P(8), and G4P(8) (0.9%); G2P(6), G3P(9), G4P(6), and G9P(6) (0.6%); and G4P(4) (0.3%). G3G1P(8) (5.2%), G1G2P(4) (0.9%), G1G9P(8) (0.6%), and G2P(4)P(8) (0.3%) were mixed types. This study documents the molecular epidemiology, genetics, and diversity of rotavirus strains in children under 5 years of age in Kunming, China, and suggests that it may be important to offer RVA vaccination and scientific evidence to stop and control RVA-related diarrhea.

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“…This study provides further evidence that RVA is a cause of acute adult diarrhea in China, but other study show that RVA was not an etiological agent with diarrhea [32]. The frequency of RVA infection (7.4%) was close to other study (9.6%) [39], but was higher than in the study (2.6%) conducted in adolescents or adults (10–89 years) in Italy [40]. …”

Section: Discussionsupporting

confidence: 69%

Case–control study of diarrheal disease etiology in individuals over 5 years in southwest China

Zhang

Yang

et al. 2016

Gut Pathog

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BackgroundAcute diarrhea is one of the major public health problems worldwide. Most of studies on acute diarrhea have been made on infants aged below 5 years and few efforts have been made to identify the etiological agents of acute diarrhea in people over five, especially in China.Methods271 diarrhea cases and 149 healthy controls over 5 years were recruited from four participating hospitals between June 2014 and July 2015. Each stool specimen was collected to detect a series of enteric pathogens, involving five viruses (Rotavirus group A, RVA; Norovirus, NoV; Sapovirus, SaV; Astrovirus, As; and Adenovirus, Ad), seven bacteria (diarrheagenic Escherichia coli, DEC; non-typhoidal Salmonella, NTS; Shigella spp.; Vibrio cholera; Vibrio parahaemolyticus; Aeromonas spp.; and Plesiomonas spp.) and three protozoa (Cryptosporidium spp., Giardia lamblia, G. lamblia, and Blastocystis hominis, B. hominis). Standard microbiological and molecular methods were applied to detect these pathogens. Data was analyzed using Chi square, Fisher-exact tests and logistic regressions.ResultsThe prevalence of at least one enteric pathogen was detected in 29.2% (79/271) acute diarrhea cases and in 12.1% (18/149) in healthy controls (p < 0.0001). Enteric viral infections (14.4%) were the most common in patients suffering from acute diarrhea, followed by bacteria (13.7%) and intestinal protozoa (4.8%). DEC (12.5%) was the most common causative agent in diarrhea cases, followed by NoV GII (10.0%), RVA (7.4%) and B. hominis (4.8%). The prevalence of co-infection was statistically higher (p = 0.0059) in the case group (7.7%) than in the healthy control (1.3%). RVA–NoV GII (3.0%) was the most common co-infection in symptomatic cases.ConclusionsDEC was the most predominant pathogen in diarrhea cases, but it was largely overlooked because the lack of laboratory capacities. Because of the high prevalence of co-infections, it is recommended the urgent development of alternative laboratory methods to assess polymicrobial infections. Such methodological improvements will result in a better prevention and treatment strategies to control diarrhea illness in China.Electronic supplementary materialThe online version of this article (doi:10.1186/s13099-016-0141-1) contains supplementary material, which is available to authorized users.

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Molecular analysis of group A rotaviruses detected in adults and adolescents with severe acute gastroenteritis in Italy in 2012

Cited by 14 publications

References 32 publications

Prevalence of Rotavirus Genotypes in Children Younger than 5 Years of Age before the Introduction of a Universal Rotavirus Vaccination Program: Report of Rotavirus Surveillance in Turkey

Prevalence of Rotavirus Genotypes in Children Younger than 5 Years of Age before the Introduction of a Universal Rotavirus Vaccination Program: Report of Rotavirus Surveillance in Turkey

Epidemiology and Genetic Diversity of Rotavirus in Kunming, China, in 2015

Case–control study of diarrheal disease etiology in individuals over 5 years in southwest China

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