Molecular analysis of H&amp;E- and Papanicolau-stained samples—systematic review

Pote, Alexandra; Boghenco, Otília; Marques-Ramos, Ana

doi:10.1007/s00418-020-01882-w

Cited by 12 publications

(9 citation statements)

References 79 publications

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“…Previous studies have investigated the yield of DNA extracted from H&E, using different protocols of extraction. Although DNA extracted from H&E showed lower recovery and some degree of fragmentation compared to unstained starting samples, however, it has proven to be adequate to perform PCR with high efficiency [10,11]. Several studies evaluated DNA extracted also from archived Papanicolaou-stained smears of lung adenocarcinoma demonstrating that this stain does not influence DNA extraction, allowing for a successful mutational analysis by PCR-based and NGS [12][13][14].…”

Section: Discussionmentioning

confidence: 99%

A Critical Issue in Lung Cancer Cytology and Small Biopsies: DNA and RNA Extraction from Archival Stained Slides for Biomarker Detection through Real Time PCR and NGS—The Experience in Pathological Anatomy Unit

et al. 2023

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The handling of biomaterials is crucial for precision medicine in advanced-stage lung patients with only cytology or small biopsies available. The main purpose of the study was to evaluate the quantity and quality of nucleic acids extracted from mixed stained slides (MSSs), including H&E, IHC and FISH, compared to the extraction from unstained slides (USs). A series of 35 lung adenocarcinoma surgical samples was selected to set up the method and the technical approach was validated in a series of 15 small biopsies and 38 cytological samples. DNA extracted from MSSs was adequate in all samples and the Real Time PCR was successful in 30/35 surgical samples (86%), 14/15 small biopsies (93%), and 33/38 cytological samples (87%). NGS using DNA extracted from MSSs was successful in 18/35 surgical samples (51%), 11/15 small biopsies (73%), and 26/38 cytological samples (68%). RNA extracted from MSSs was unsatisfactory in all cases showing an inadequate degree of fragmentation. Our technical approach based on the recovery of stained slides could represent a strategic way forward for DNA-based biomarker testing in lung cancer cases without biomaterials. The RNA extracted from MSSs did not represent a successful approach.

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Section: Discussionmentioning

confidence: 99%

A Critical Issue in Lung Cancer Cytology and Small Biopsies: DNA and RNA Extraction from Archival Stained Slides for Biomarker Detection through Real Time PCR and NGS—The Experience in Pathological Anatomy Unit

et al. 2023

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“…The main advantage of Pap staining is that it clearly shows the fine structure of the nucleus. The cytoplasm is bright and transparent, reflecting the change in the differentiation of the cytoplasm (44,45). Diff-Quick staining is a fast staining method modified on the basis of Swiss staining.…”

Section: Cytopathological Specimen Collectionmentioning

confidence: 99%

Minimally Invasive Cytopathology and Accurate Diagnosis: Technical Procedures and Ancillary Techniques

Huang

Wang

et al. 2023

In Vivo

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In recent years, the demand for cytopathological accurate diagnoses has increased as expanding minimally invasive procedures obtain materials from patients with advanced cancer for diagnostic, prognostic, and predictive purposes. However, inadequate knowledge of cytopathological technical procedures and ancillary techniques by clinicians remains the most common reason for the limited availability of cytopathology. The objectives of this review were to understand the technical procedures, ancillary techniques, and application and effectiveness of various types of tests in cytopathology. Each of the many ancillary technologies described in the literature has specific advantages and limitations and laboratories select one or more methods depending on their infrastructure and expertise to achieve the goal from initial screening of the disease to the final diagnosis of the cytopathology. This paper systematically reviews the development of cytopathology, summarizes the existing problems in cytopathology and the new progress of auxiliary examination, to provide a theoretical basis for the advanced development of cytopathological diagnostic technologies and to consolidate the minimally invasive and accurate diagnosis of cytopathologies for clinicians.Cytopathology offers many advantages over other clinical examinations, particularly for minimally invasive and accurate diagnosis.

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“…H&E and Papanicolau (Pap) staining result in low DNA recovery and some degree of DNA fragmentation. [ 41 ] To overcome these limitations the usage of consecutive stained and unstained slides or the adaption of the staining protocols by reducing incubation times and solvent concentration are appropriate. Another option for performing cell selection and analysis on the same but unstained slide is offered in novel AI approaches for automatical tissue classification (see Section 5).…”

Section: Omic Techniques Compatible With Microdissectionmentioning

confidence: 99%

Microdissection—An Essential Prerequisite for Spatial Cancer Omics

Eggeling

Hoffmann

2020

Proteomics

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The problem with cancer tissue is that its intratumoral heterogeneity and its complexity is extremely high as cells possess, depending on their location and function, different mutations, different mRNA expression and the highest intricacy in the protein pattern. Prior to genomic and proteomic analyses, it is therefore indispensable to identify the exact part of the tissue or even the exact cell. Laser‐based microdissection is a tried and tested technique able to produce pure and well‐defined cell material for further analysis with proteomic and genomic techniques. It sheds light on the heterogeneity of cancer or other complex diseases and enables the identification of biomarkers. This review aims to raise awareness for the reconsideration of laser‐based microdissection and seeks to present current state‐of‐the‐art combinations with omic techniques.

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Molecular analysis of H&E- and Papanicolau-stained samples—systematic review

Cited by 12 publications

References 79 publications

A Critical Issue in Lung Cancer Cytology and Small Biopsies: DNA and RNA Extraction from Archival Stained Slides for Biomarker Detection through Real Time PCR and NGS—The Experience in Pathological Anatomy Unit

A Critical Issue in Lung Cancer Cytology and Small Biopsies: DNA and RNA Extraction from Archival Stained Slides for Biomarker Detection through Real Time PCR and NGS—The Experience in Pathological Anatomy Unit

Minimally Invasive Cytopathology and Accurate Diagnosis: Technical Procedures and Ancillary Techniques

Microdissection—An Essential Prerequisite for Spatial Cancer Omics

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