Molecular analysis of <i>Malassezia</i> species isolated from Israeli patients with pityriasis versicolor

Lyakhovitsky, Anna; Shemer, Avner; Amichai, Boaz

doi:10.1111/j.1365-4632.2012.05595.x

Cited by 12 publications

(8 citation statements)

References 14 publications

(54 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…M . furfur has been shown to be rare or even absent, and when detected, is found in low proportions by molecular-based studies [ 3 , 12 , 47 – 50 ]. In contrast, it is often isolated by culture-based methods [ 51 – 53 ], which favor faster-growing organisms like M .…”

Section: Discussionmentioning

confidence: 99%

Malassezia Intra-Specific Diversity and Potentially New Species in the Skin Microbiota from Brazilian Healthy Subjects and Seborrheic Dermatitis Patients

et al. 2015

View full text Add to dashboard Cite

Malassezia yeasts are part of the resident cutaneous microbiota, and are also associated with skin diseases such as seborrheic dermatitis (SD). The role these fungi play in skin diseases and why they are pathogenic for only some individuals remain unclear. This study aimed to characterize Malassezia microbiota from different body sites in healthy and SD subjects from Brazil. Scalp and forehead samples from healthy, mild SD and severe SD subjects were collected. Non-scalp lesions from severe SD patients were also sampled. 5.8S rDNA/ITS2 amplicons from Malassezia sp. were analyzed by RFLP and sequencing. Results indicate that Malassezia microbiota did not group according to health condition or body area. Phylogenetic analysis revealed that three groups of sequences did not cluster together with any formally described species, suggesting that they might belong to potential new species. One of them was found in high proportions in scalp samples. A large variety of Malassezia subtypes were detected, indicating intra-specific diversity. Higher M. globosa proportions were found in non-scalp lesions from severe SD subjects compared with other areas, suggesting closer association of this species with SD lesions from areas other than scalp. Our results show the first panorama of Malassezia microbiota in Brazilian subjects using molecular techniques and provide new perspectives for further studies to elucidate the association between Malassezia microbiota and skin diseases.

show abstract

Section: Discussionmentioning

confidence: 99%

Malassezia Intra-Specific Diversity and Potentially New Species in the Skin Microbiota from Brazilian Healthy Subjects and Seborrheic Dermatitis Patients

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Few studies found that M. pachydermatis may be transmitted to humans from pets [7,10]. M. pachydermmatis is the only lipid independent species while others are lipid dependent [11].…”

Section: Introductionmentioning

confidence: 99%

Molecular and Phenotypic Identification and Speciation of Malassezia Yeasts Isolated from Egyptian Patients with Pityriasis Versicolor

Elshabrawy

Saudy

Sallam

2017

JCDR

View full text Add to dashboard Cite

show abstract

“…Malassezia species on both healthy (Findley et al, 2013) and diseased skin, such as pityriasis versicolor and seborrheic dermatitis ( Prohic & Ozegovic, 2007;Prohic, 2010;Amado et al, 2013;Lyakhovitsky et al, 2013;Lian et al, 2014;Rodoplu et al, 2014). M. sympodialis is the most frequent coloniser of healthy skin (Findley et al, 2013), but is found less frequently in Malassezia related-diseases, such as seborrheic dermatitis and pityriasis versicolour (Prohic & Ozegovic, 2007;Prohic, 2010;Amado et al, 2013).…”

Section: Globosa M Furfur M Restricta and M Sympodialis Are Tmentioning

confidence: 99%

Host responses in anex-vivohuman skin model challenged withMalassezia sympodialis

Corzo-León

MacCallum

Munro

2020

Preprint

View full text Add to dashboard Cite

Malassezia species are a major part of the normal mycobiota and colonise mainly sebum-rich skin regions of the body. This group of fungi cause a variety of infections such as pityriasis versicolour, folliculitis and fungaemia. In particular, Malassezia sympodialis and its allergens have been associated with non-infective inflammatory diseases such as seborrheic dermatitis and atopic eczema. The aim of this study was to investigate the host response to M. sympodialis on oily skin (supplemented with oleic acid) and non-oily skin using an ex-vivo human skin model. Host-pathogen interactions were analysed by SEM, histology, gene expression, immunoassays and dual species proteomics. The skin response to M. sympodialis was characterised by increased expression of the genes encoding β-defensin 3 and RNase7, and by high levels of S100 proteins in tissue. Supplementation of oleic acid onto skin was associated with direct contact of yeasts with keratinocytes and epidermal damage. In oily conditions, skin response to M. sympodialis showed no gene expression of AMPs, but increased expression of IL18. In supernatants from inoculated skin plus oleic acid, TNFα levels were decreased and IL-18 levels were significantly increased.

show abstract

Molecular analysis of Malassezia species isolated from Israeli patients with pityriasis versicolor

Abstract: Based on the PCR test, we have shown that M. globosa is probably most frequently associated with PV.

Cited by 12 publications

References 14 publications

Malassezia Intra-Specific Diversity and Potentially New Species in the Skin Microbiota from Brazilian Healthy Subjects and Seborrheic Dermatitis Patients

Malassezia Intra-Specific Diversity and Potentially New Species in the Skin Microbiota from Brazilian Healthy Subjects and Seborrheic Dermatitis Patients

Molecular and Phenotypic Identification and Speciation of Malassezia Yeasts Isolated from Egyptian Patients with Pityriasis Versicolor

Host responses in anex-vivohuman skin model challenged withMalassezia sympodialis

Contact Info

Product

Resources

About