Molecular Analysis of<i>Plasmodium ovale</i>Variants

Win, Thin Thida; Jalloh, Amadu; Tantular, Indah Setyawati; Tsuboi, Takafumi; Ferreira, Marcelo Urbano; Kimura, Michio; Kawamoto, Fumihiko

doi:10.3201/eid1007.030411

Cited by 64 publications

(73 citation statements)

References 29 publications

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“…32,41 On the basis of analysis of sequences from three different P. ovale genes, a recent report segregates P. ovale into two clades, classic and variant, that are as divergent as separate species. 31 Analysis of partial 18S rRNA gene sequences from the two P. ovale isolates described here indicates they are both members of the classic clade (data not shown). It would be interesting to obtain the MSP1 sequence for a member of the P. ovale variant clade, and compare it to the sequences presented here to see if it supports the contention that the two clades represent separate species.…”

Section: Discussionmentioning

confidence: 81%

“…31 BLAST analysis showed 100% homology of the amplicon sequences with "classic-type" P. ovale 18S rRNA gene sequences in GenBank (data not shown). For specimens identified as co-infected with P. falciparum and P. malariae , the 18S rRNA gene sequence chromatograms suggested the presence of multiple sequences.…”

Section: Identification Of Parasitemic Donors Whole Blood Frommentioning

confidence: 99%

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Isolation and Characterization of the MSP1 Genes from Plasmodium malariae and Plasmodium ovale

Birkenmeyer¹,

Muerhoff²,

Dawson³

et al. 2010

The American Society of Tropical Medicine and Hygiene

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The merozoite surface protein 1 (MSP1) is the principal surface antigen of the blood stage form of the Plasmodium parasite. Antibodies recognizing MSP1 are frequently detected following Plasmodium infection, making this protein a significant component of malaria vaccines and diagnostic tests. Although the MSP1 gene sequence has been reported for Plasmodium falciparum and Plasmodium vivax, this gene has not been identified for the other two major human-infectious species, Plasmodium malariae and Plasmodium ovale. MSP1 genes from these two species were isolated from Cameroon blood donor samples. The genes are similar in size to known MSP1 genes and encode proteins with interspecies conserved domains homologous to those identified in other Plasmodium species. Sequence and phylogenetic analysis of all available Plasmodium MSP1 amino acid sequences clearly shows that the Po and Pm MSP1 sequences are truly unique within the Plasmodium genus and not simply Pf or Pv variants.

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Section: Discussionmentioning

confidence: 81%

Section: Identification Of Parasitemic Donors Whole Blood Frommentioning

confidence: 99%

Isolation and Characterization of the MSP1 Genes from Plasmodium malariae and Plasmodium ovale

Birkenmeyer¹,

Muerhoff²,

Dawson³

et al. 2010

The American Society of Tropical Medicine and Hygiene

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“…Sensitive molecular techniques that allow the sensitive detection and the accurate identification of Plasmodium parasites, on the basis of amplification of their small-subunit rRNA (ssrRNA) gene, revealed that the prevalences of P. malariae and P. ovale infections were higher than previously thought (9,14,18,21,25). Sequence variations were, however, detected in these genes in P. malariae and, in particular, in P. ovale, in which two types were found (4,8,28,30). This led to the design of novel oligonucleotide primer combinations to increase the accuracy of P. ovale detection (5,10,19).…”

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confidence: 99%

“…The efficacy and the accuracy of detection rely on a lack of variation in the target sequences to which the amplification primers hybridize. To date, evidence of polymorphisms in the ssrRNA genes was obtained for P. ovale and P. malariae (4,8,28,30), mainly isolates collected in Southeast Asia, but not for P. falciparum and P. vivax. This might reflect a different and possibly more ancient evolutionary pathway for P. malariae and P. ovale.…”

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confidence: 99%

Genetic Polymorphisms Influence Plasmodium ovale PCR Detection Accuracy

et al. 2007

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“…Kodak BioMax MS Films were obtained from Perkin Elmer (France). In addition, intensifying screens (Kodak BioMax TranScreen LE, Perkin Elmer) were used for TLC of [1][2][3] H]ethanolamine-labeled metabolites. Radioactive spots were scraped off directly into scintillation vials and radioactivity was determined by liquid scintillation counting (Ultimagold, Perkin Elmer) and counted in a Beckman LS 6500 spectrometer.…”

Section: Syntenic Regions Corresponding To the Genomic Context Of Phomentioning

confidence: 99%

Rodent and nonrodent malaria parasites differ in their phospholipid metabolic pathways

Déchamps

Maynadier

Wein

et al. 2010

Journal of Lipid Research

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Malaria is a disease caused by an intraerythrocytic protozoan parasite of the genus Plasmodium , which is transmitted by dipterans and affects vertebrates such as reptiles, birds, and mammals, including humans (see supplementary Table I). By contrast with other Apicomplexa parasite species that can infect a broad range of metazoans, Plasmodium species have a narrow specifi city range regarding insect and vertebrate hosts ( 1 ). Plasmodium falciparum is, thus, responsible for the most severe form of malaria in humans only. Other species, such as P. vivax , two P. ovale subspecies ( 2 ), P. malariae , and, according to recent reports, P. knowlesi ( 3 ) cause less complicated forms of human malaria. The host specifi city of P. knowlesi is not restricted to humans because it also infects monkeys.The evolutionary history of Plasmodium species has been highly debated, especially the position of P. falciparum , either grouped with avian parasites ( 4, 5 ) or placed as a sister species to other mammalian parasites including rodent parasites. The fi ndings of most recent analyses using three classes of rare genomic changes and mitochondrial RNA genes unambiguously support a mammalian clade and no Abstract Malaria, a disease affecting humans and other animals, is caused by a protist of the genus Plasmodium . At the intraerythrocytic stage, the parasite synthesizes a high amount of phospholipids through a bewildering number of pathways. In the human Plasmodium falciparum species, a plant-like pathway that relies on serine decarboxylase and phosphoethanolamine N-methyltransferase activities diverts host serine to provide additional phosphatidylcholine and phosphatidylethanolamine to the parasite. This feature of parasitic dependence toward its host was investigated in other Plasmodium species. In silico analyses led to the identifi cation of phosphoethanolamine N-methyltransferase gene orthologs in primate and bird parasite genomes. However, the gene was not detected in the rodent P. berghei , P. yoelii , and P. chabaudi species. Biochemical experiments with labeled choline, ethanolamine, and serine showed marked differences in biosynthetic pathways when comparing rodent P. berghei and P. vinckei , and human P. falciparum species. Notably, in both rodent parasites, ethanolamine and serine were not signifi cantly incorporated into phosphatidylcholine, indicating the absence of phosphoethanolamine N-methyltransferase activity. To our knowledge, this is the fi rst study to highlight a crucial difference in phospholipid metabolism between Plasmodium species. The fi ndings should facilitate efforts to develop more rational approaches to identify and evaluate new targets for antimalarial therapy.

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Molecular Analysis ofPlasmodium ovaleVariants

Abstract: Sequence analyses of six isolates from Southeast Asia supports dividing species into types

Cited by 64 publications

References 29 publications

Isolation and Characterization of the MSP1 Genes from Plasmodium malariae and Plasmodium ovale

Isolation and Characterization of the MSP1 Genes from Plasmodium malariae and Plasmodium ovale

Genetic Polymorphisms Influence Plasmodium ovale PCR Detection Accuracy

Rodent and nonrodent malaria parasites differ in their phospholipid metabolic pathways

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