Molecular analysis of nosocomial infection by oxacillin-resistant Staphylococcus aureus lacking protein A and clumping factor

Schwarzkopf, Andreas; Schmidt-Rotte, H.; Schmidt, Herbert; E, Kunz; Karch, Helge; Heesemann, Jürgen

doi:10.1016/0140-6736(92)92165-c

Cited by 5 publications

(2 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…aureus is the clumping factor and protein A detection performed with latex particles coated with fibrinogen or immunoglobulin G (IgG). Unusual strains of ORSA which were nonreactive in the two latex agglutination assays have recently been reported in North America (20), the British Isles (13), and Germany (18). After isolating clumping factornegative (CFN) ORSA from a hospital outbreak (18), we learned of five other hospitals in Germany which had also recovered such unusual outbreak-associated CFN ORSA strains.…”

mentioning

confidence: 99%

Phenotypical and genotypical characterization of epidemic clumping factor-negative, oxacillin-resistant Staphylococcus aureus

Schwarzkopf¹,

Karch²,

Schmidt³

et al. 1993

J Clin Microbiol

View full text Add to dashboard Cite

A total of 50 oxacillin-resistant Staphylococcus aureus (ORSA) strains that were clumping factor negative (CFN) and protein A negative by latex agglutination were collected from patients in six different hospitals at different locations in Germany during 199l and 1992. Antibiograms, bacteriophage typing, and plasmid analysis were performed. The antibiograms showed that, besides oxacillin, all CFN ORSA strains were resistant to gentamicin, clindamycin, erythromycin, ciprofloxacin, and fosfomycin. All these isolates were nontypeable with an international set of phages, and an additional experimental phage set indicated that the strains were phage type 16, 192. Moreover, all isolates possessed a single plasmid of 30 kb, and restriction analysis of those plasmids revealed identical patterns. For genotyping, these 50 isolates were also analyzed by pulsed-field gel electrophoresis (PFGE) and polymerase chain reaction (PCR) of the coagulase and protein A genes and then by restriction enzyme digestion and analysis of restriction fragment length polymorphisms (RFLPs). With 49 strains, electrophoresis of SmaI-digested chromosomal DNA revealed identical PFGE patterns regarding the number and size of the DNA fragments, which could be differentiated from those of clumping factor-positive ORSA strains. Typing for the coagulase gene by PCR revealed PCR products of identical sizes. The AluI restriction digestion patterns of the PCR products were identical. PCR with primers derived from the region of that part of the protein A gene that encodes the immunoglobulin G-binding domains showed a PCR product that was about 170 bp smaller than that of the protein A gene from strains that were positive in the protein A latex agglutination test. Since it is precisely this size that is required in order to encode one immunoglobulin G-binding region, we assume that this is not present in the CFN ORSA strains. The * Corresponding author.

show abstract

mentioning

confidence: 99%

Phenotypical and genotypical characterization of epidemic clumping factor-negative, oxacillin-resistant Staphylococcus aureus

Schwarzkopf¹,

Karch²,

Schmidt³

et al. 1993

J Clin Microbiol

View full text Add to dashboard Cite

show abstract

“…In addition to S. lugdunensis and S. schleiferi, rare strains of S. aureus have been reported to produce only clumping factor and DNase, but not free coagulase [6,[9][10][11][12][13][14]. Also S. aureus variants lacking clumping factor [12,13], clumping factor and protein A [14][15][16][17] and catalase production [18,19] have been reported. These reports suggest that although the coagulase-negative variants of S. aureus are considered to be rare, their numbers appear to be increasing.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of Coagulase-Negative Methicillin-Resistant <i>Staphylococcus aureus</i> from Patients in an Intensive Care Unit

et al. 1999

View full text Add to dashboard Cite

Objective: To report the isolation and characterization of four coagulase-negative methicillin-resistant Staphylococcus aureus isolated from patients admitted to the ICU of a Kuwait hospital by bacteriological and molecular methods. Methods: The isolates were characterized by cultural characteristics, Gram stain, catalase, coagulase, DNase and biochemical tests and typed by a combination of antibiogram and pulsed-field gel electrophoresis to evaluate their relatedness. Results: These isolates were gram-positive cocci in clusters, catalase, DNase and slide coagulase-positive but tube coagulase-negative. They gave negative results for ornithine decarboxylase and pyrrolidonyl-arylamidase tests, which indicated that these were not Staphylococcus lugdunensis or Staphylococcus schleiferi, and were identified as S. aureus by API Staph. They had identical antibiograms and pulsed-field gel electrophoresis patterns which suggested that they had a common origin. Conclusion: The results highlight the need to complement the coagulase tests with other tests such as DNase and biochemical tests to correctly identify S. aureus. Coagulase-negative S. aureus appears to be an increasing problem that clinical laboratories should be aware of. They are as virulent as those producing coagulase and can colonize, cause infections and spread among patients.

show abstract

Genomic Fingerprinting for the Evaluation of Epidemic Processes

Schwarzkopf¹,

Böhm²,

Schmidt³

et al. 1995

Immunology and Its Impact on Infections in Surgery

View full text Add to dashboard Cite

Molecular analysis of nosocomial infection by oxacillin-resistant Staphylococcus aureus lacking protein A and clumping factor

Cited by 5 publications

References 3 publications

Phenotypical and genotypical characterization of epidemic clumping factor-negative, oxacillin-resistant Staphylococcus aureus

Phenotypical and genotypical characterization of epidemic clumping factor-negative, oxacillin-resistant Staphylococcus aureus

Isolation and Characterization of Coagulase-Negative Methicillin-Resistant <i>Staphylococcus aureus</i> from Patients in an Intensive Care Unit

Genomic Fingerprinting for the Evaluation of Epidemic Processes

Contact Info

Product

Resources

About