Molecular analysis of sporulation in Streptomyces griseus

McCue, Lee Ann; Kwak, Jae; Babcock, Martin J.; Kendrick, Kathleen E.

doi:10.1016/0378-1119(92)90556-5

Cited by 33 publications

(19 citation statements)

References 30 publications

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“…In S. coelicolor, bldA specifying this tRNA controls both aerial mycelium formation and secondary metabolite formation at the translational level (9,30). This suggests that the expression of amfR is regulated by the bldA gene, which appears to control morphogenesis in S. griseus (32) It is also possible that other species do not use a system like the amfR-amfA/am/B system and that there are diverse peripheral routes to the activation of the core developmental genes. In relation to this, A-factor itself exerts its regulatory function only in limited species, although there is a possibility that -y-butyrolactones structurally similar to A-factor serve as hormonal regulators in general in a given Streptomyces sp.…”

Section: Discussionmentioning

confidence: 99%

A gene cluster involved in aerial mycelium formation in Streptomyces griseus encodes proteins similar to the response regulators of two-component regulatory systems and membrane translocators

et al. 1993

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Section: Discussionmentioning

confidence: 99%

A gene cluster involved in aerial mycelium formation in Streptomyces griseus encodes proteins similar to the response regulators of two-component regulatory systems and membrane translocators

et al. 1993

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“…The orf1590 gene was originally reported by Babcock and Kendrick (1) to be a gene complementing a Bld phenotype of an S. griseus mutant. The expression of orf1590 appears to be very complex, although the mechanism by which it controls aerial mycelium formation is still unknown (25). The same gene was also cloned by our group (31) as a 2.8-kb BamHI fragment on pIJ702 (plasmid pSPO5) that restored the aerial mycelium-negative phenotype of the A-factor-deficient mutant strain, HH1.…”

Section: Figmentioning

confidence: 99%

Cloning and characterization of a gene involved in aerial mycelium formation in Streptomyces griseus

et al. 1995

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, S. griseus HH1, was cloned from this strain on a high-copy-number plasmid. Subcloning and nucleotide sequencing revealed that one open reading frame with 218 amino acids, named AmfC, served as a multicopy suppressor of the aerial mycelium-defective phenotype of the A-factor-deficient strain. The amfC gene did not restore A-factor or streptomycin production, indicating that amfC is involved in aerial mycelium formation independently of secondary metabolic function. Disruption of the chromosomal amfC gene in the wild-type S. griseus strain caused a severe reduction in the abundance of spores but no effect on the shape or size of the spores. The infrequent sporulation of the amfC disruptant was reversed by introduction of amfC on a plasmid. The amfC-defective phenotype was also restored by the orf1590 gene but not by the amfR-amfA-amfB gene cluster. Nucleotide sequences homologous to the amfC gene were distributed in all of 12 Streptomyces species tested, including Streptomyces coelicolor A3(2). The amfC homolog of S. coelicolor A3(2) was cloned and its nucleotide sequence was determined. The AmfC products of S. griseus and S. coelicolor A3(2) showed a 60% identity in their amino acid sequences. Introduction of the amfC gene of S. coelicolor A3(2) into strain HH1 induced aerial mycelium formation and sporulation, which suggests that both play the same functional role in morphogenesis in the strains.

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“…The tDNAs from all four species are missing the invariant and semi-invariant bases 18G and 21A (Lawlor et al, 1987 ;McCue et al, 1992 ;Ueda et al, 1992). Since other tDNAs, such as S. coelicolor leuα and leuβ, S. lividans cysT (Sedlmeier & Schmieger, 1990) and tDNA Asp GTC (Sedlmeier et al, 1993) are also missing 21A, its absence is not uncommon, although it may be a feature of Streptomyces leucyl-tRNAs as all those identified to date are missing this nucleotide.…”

Section: Discussionmentioning

confidence: 92%

“…Since this non-random distribution of TTA codons is conserved among Streptomyces species, it suggests that the bldA tRNA may perform a similar role in most, if not all, members of the genus. At present, the bldA gene has been identified in Streptomyces lividans (Ueda et al, 1992), which is closely related to S. coelicolor, as well as in Streptomyces griseus (McCue et al, 1992), and is similarly implicated in sporulation and antibiotic production in these two species.…”

Section: Introductionmentioning

confidence: 99%

The positive activator of cephamycin C and clavulanic acid production in Streptomyces clavuligerus is mistranslated in a bldA mutant The GenBank accession number for the sequence reported in this paper is AF436078.

et al. 2002

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In Streptomyces coelicolor bldA encodes the principal leucyl tRNA for translation of UUA codons and controls pigmented antibiotic production by the presence of TTA codons in the genes encoding the pathway-specific activators of actinorhodin and undecylprodigiosin biosynthesis. In Streptomyces clavuligerus the gene encoding the pathway-specific activator of both cephamycin C and clavulanic acid production, ccaR, also contains a TTA codon and was expected to exhibit bldA-dependence. A cloned S. clavuligerus DNA fragment containing a sequence showing 91 % identity to the S. coelicolor bldA-encoded tRNA was able to restore antibiotic production and sporulation to bldA mutants of S. coelicolor and the closely related Streptomyces lividans. A null mutation of the bldA gene in S. clavuligerus resulted in the expected sporulation defective phenotype, but unexpectedly had no effect on antibiotic production. Transcript analysis showed no difference in the levels of ccaR transcripts in the wild-type and bldA mutant strains, ruling out any effect of elevated levels of the ccaR mRNA. Furthermore, when compared to the wildtype strain, the bldA mutant showed no differences in the levels of CcaR, suggesting that the single TTA codon in ccaR is mistranslated efficiently. The role of codon context in bldA dependence is discussed.

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Molecular analysis of sporulation in Streptomyces griseus

Cited by 33 publications

References 30 publications

A gene cluster involved in aerial mycelium formation in Streptomyces griseus encodes proteins similar to the response regulators of two-component regulatory systems and membrane translocators

A gene cluster involved in aerial mycelium formation in Streptomyces griseus encodes proteins similar to the response regulators of two-component regulatory systems and membrane translocators

Cloning and characterization of a gene involved in aerial mycelium formation in Streptomyces griseus

The positive activator of cephamycin C and clavulanic acid production in Streptomyces clavuligerus is mistranslated in a bldA mutant The GenBank accession number for the sequence reported in this paper is AF436078.

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