Molecular analysis of the determination of developmental fate in the small intestinal epithelium in the chicken embryo

Hiramatsu, Hiroaki; Yasugi, Sadao

doi:10.1387/ijdb.041886hh

Cited by 9 publications

(9 citation statements)

References 38 publications

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“…In chick, onset of neurogenesis in most embryonic structures takes place around this time and continues till HH27 [42]–[44]. While induction and early patterning of the gut tube in chick is completed before HH18 differentiation begins around HH30 [45]. We have observed the peak of MRG expression in the gut at HH28 (Fig.…”

Section: Discussionmentioning

confidence: 54%

A Genome-Wide Screen Indicates Correlation between Differentiation and Expression of Metabolism Related Genes

et al. 2013

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Differentiated tissues may be considered as materials with distinct properties. The differentiation program of a given tissue ensures that it acquires material properties commensurate with its function. It may be hypothesized that some of these properties are acquired through production of tissue-specific metabolites synthesized by metabolic enzymes. To establish correlation between metabolism and organogenesis we have carried out a genome-wide expression study of metabolism related genes by RNA in-situ hybridization. 23% of the metabolism related genes studied are expressed in a tissue-restricted but not tissue-exclusive manner. We have conducted the screen on whole mount chicken (Gallus gallus) embryos from four distinct developmental stages to correlate dynamic changes in expression patterns of metabolic enzymes with spatio-temporally unique developmental events. Our data strongly suggests that unique combinations of metabolism related genes, and not specific metabolic pathways, are upregulated during differentiation. Further, expression of metabolism related genes in well established signaling centers that regulate different aspects of morphogenesis indicates developmental roles of some of the metabolism related genes. The database of tissue-restricted expression patterns of metabolism related genes, generated in this study, should serve as a resource for systematic identification of these genes with tissue-specific functions during development. Finally, comprehensive understanding of differentiation is not possible unless the downstream genes of a differentiation cascade are identified. We propose, metabolic enzymes constitute a significant portion of these downstream target genes. Thus our study should help elucidate different aspects of tissue differentiation.

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Section: Discussionmentioning

confidence: 54%

A Genome-Wide Screen Indicates Correlation between Differentiation and Expression of Metabolism Related Genes

et al. 2013

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“…In the epithelium of the developing chicken SI before day 15 of incubation, Msi‐1 is expressed ubiquitously. Cytodifferentiation of the SI epithelium begins from around day 7 to day 9 of incubation, when the expression of chicken intestinal fatty acid binding proteins and sucrase commences (Hiramatsu & Yasugi 2004). The ubiquitous expression of Msi‐1 may therefore be related to the onset of such SI‐specific gene expression.…”

Section: Discussionmentioning

confidence: 99%

Correlation between Musashi‐1 and c‐hairy‐1 expression and cell proliferation activity in the developing intestine and stomach of both chicken and mouse

2005

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Musashi-1 (Msi-1) is an RNA-binding protein that plays key roles in the maintenance of neural stem cell states and in their differentiation into neural cells. Msi-1 has also been proposed as a candidate marker gene of mammalian intestinal stem cells and their immediate lineages. In this study, we examined Msi-1 expression in the small intestine and the stomach of both chicken and mouse during embryonic, fetal and postnatal development. In addition, we analyzed the expression of c-hairy-1, a chicken homologue of mouse Hes1, and assessed the proliferative activity of the cells expressing both of these factors. Significantly, during the development of these digestive organs in both species Msi-1 expression showed dynamic changes, suggesting that it is important for digestive organ development, particularly for epithelial differentiation. Based on our observations of the expression patterns of Msi-1 and c-hairy-1 in the adult small intestine, we speculate that Msi-1 is also a stem cell marker of the chicken small intestinal epithelium.

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“…If the endoderm was cultivated with dermis taken from 6 day old embryonic skin, it expressed Shh , a pan‐endodermal marker, but not specific markers of either the intestinal or stomach epithelium. These data indicated that the young presumptive intestinal endoderm possesses a differentiation potency towards intestinal epithelium, but that the realization of this is dependent on certain factors from the digestive organ mesenchyme or splanchnic mesoderm (Hiramatsu & Yasugi 2004). Although we have not yet elucidated the factors that are involved in the specification of the anterior and posterior parts of the primitive digestive tract, it is highly conceivable that CdxA , a homologue of the Drosophila caudal gene, is important.…”

Section: Molecular Mechanisms Of Digestive Organ Developmentmentioning

confidence: 99%

Molecular analysis of endoderm regionalization

Yasugi¹,

Mizuno²

2008

Dev Growth Differ

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We have engaged in a number of studies in our laboratory that have focused on the molecular mechanisms underlying gut formation, with particular attention being paid to the establishment of regional differences found in the entire gut and within each digestive organ. We have found from our analyses that the presumptive fate of the endoderm in the embryos of vertebrates is determined quite early during development, but the realization of this fate often requires molecular cues from the neighboring tissues such as the lateral plate mesoderm and the mesenchyme derived from it. The mesenchyme seems often to exert instructive or supportive induction effects and, in some cases, a completely inhibitory role during the differentiation of the endodermal epithelium. In addition, many reports on the formation of the stomach, intestine, liver and salivary gland in vertebrates, and of Drosophila gut, all indicate that the morphogenesis and cytodifferentiation of these organs are regulated by the regulated expression of genes encoding growth factors and transcription factors. We have further shown that the epithelium can regulate the differentiation of the mesenchyme into the connective tissue and the smooth muscle layers, thus demonstrating the occurrence of literally interactive processes in the development of the digestive organs.

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Molecular analysis of the determination of developmental fate in the small intestinal epithelium in the chicken embryo

Cited by 9 publications

References 38 publications

A Genome-Wide Screen Indicates Correlation between Differentiation and Expression of Metabolism Related Genes

A Genome-Wide Screen Indicates Correlation between Differentiation and Expression of Metabolism Related Genes

Correlation between Musashi‐1 and c‐hairy‐1 expression and cell proliferation activity in the developing intestine and stomach of both chicken and mouse

Molecular analysis of endoderm regionalization

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