Molecular analysis of the gene family of the signal recognition particle (SRP) RNA of tomato

Riedel, Leonhard; Volger, Ute; Luckinger, Renate; Putz, Austin M.; Sänger, Heinz L.; Wassenegger, Michael

doi:10.1007/bf00020611

Cited by 7 publications

(6 citation statements)

References 54 publications

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“…However, a tissue-specific expression of plant 7SL RNA variants has not been studied in detail. No variability of 7SL RNA expression was reported by Riedel et al (1995Riedel et al ( , 1996 in different tissues of the tomato using molecular hybridization. However, it is possible that the hybridization technique was unable to distinguish low expression differences among 7SL RNA gene variants due to their high homology, in contrast to the specific detection method used in this study.…”

Section: Discussionmentioning

confidence: 81%

Expression of modified 7SL RNA gene in transgenic Solanum tuberosum plants

Vrba¹,

Matoušek²

2005

Biologia plant.

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A modified plant 7SL RNA gene from Arabidopsis thaliana designated AHIIA63M was introduced into potato plants via Agrobacterium-mediated transformation. No transgenic plants could be obtained using pGPTV-based binary vectors where AHIIA63M gene driven by polIII promoter was located close to the polII promoter of the selection gene. Special binary vectors with matrix attachment region (MAR) elements had to be used for transformation to insulate polII and polIII promoters within T-DNA. The level of AHIIA63M RNA in transgenic plants was lower than the levels of transcripts of transgenes driven by RNA polymerase II. The level of AHIIA63M transcript in transgenic potato plants was tissue specific. The highest expression was detected in roots and gynoecium and the lowest in tubers. Moreover, non-specific promoter activity within the MAR element was revealed. This activity contributed to AHIIA63M transcription. This is the first report of expression of a modified 7SL RNA gene in transgenic plants and promoter activity within the MAR element.Additional key words: RNA polymerase III, polIII expression cassette, MAR element.

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Section: Discussionmentioning

confidence: 81%

Expression of modified 7SL RNA gene in transgenic Solanum tuberosum plants

Vrba¹,

Matoušek²

2005

Biologia plant.

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“…Interestingly, a number of signal recognition particle (SRP) RNAs, i.e. the RNA component of the SRP ribonucleoprotein complex that targets proteins to cellular membranes (Rosenblad et al , 2009), along with small nucleolar RNA (snRNA) U3, a member of the SRP RNA family (Riedel et al , 1996), were actively regulated during the early time points (Fig. 6A).…”

Section: Resultsmentioning

confidence: 99%

“…6B). U3 snRNA, which is involved in pre-rRNA processing, is grouped together with SRP in the polymerase III-specific subclass of externally regulated poll III genes (Riedel et al , 1996). The SRP is a ribonucleoprotein complex containing an SRP RNA component, which participates in the targeting of protein to the cellular membrane (Rosenblad et al , 2009), to which the SRP recognizes signal sequences and directs secretory proteins (Andersen et al , 2006), suggesting that SRP RNA might function as a mediator controlling protein secretion at the cell membrane in response to sound wave treatment.…”

Section: Discussionmentioning

confidence: 99%

Exploring the sound-modulated delay in tomato ripening through expression analysis of coding and non-coding RNAs

Kim

Jung

et al. 2018

Annals of Botany

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“…A considerable number of apparently functional 7SL RNA genes exist in higher plant genomes such as tomato (Riedel et al. , 1996) and hop (Matoušek et al.…”

Section: Resultsmentioning

confidence: 99%

“…(b) A propeller‐like secondary structure can be formed of the first 40 nt of all known plant 7SL RNAs (Matoušek et al. , 1999; Riedel et al. , 1996).…”

Section: Resultsmentioning

confidence: 99%

Plant 7SL RNA genes belong to type 4 of RNA polymerase III‐ dependent genes that are composed of mixed promoters

et al. 2005

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SummaryThe genes transcribed by RNA polymerase III (pol III) display a great diversity in terms of promoter structure and are placed in four groups accordingly. Type 3 subset of pol III genes has promoter elements which reside entirely upstream of the coding region of the gene whereas type 4 consists of genes with mixed promoters that enclose intra-and extragenic regulatory sequences. Plant 7SL RNA genes have been previously classified as type 3 of pol III genes requiring an upstream sequence element and a canonical TATA box for transcriptional activity in transfected plant protoplasts. We have identified two novel functional control regions within the coding region of an Arabidopsis 7SL RNA gene (At7SL-1) that resemble tRNA gene-specific A and B boxes with respect to sequence and position. Single and multiple nucleotide substitutions in either of these regions resulted in a pronounced reduction of transcription activity in tobacco nuclear extract that was not caused by a decreased stability as shown by decay kinetics of wild type and mutant RNA transcripts. These findings suggest that plant 7SL RNA genes should be actually placed in type 4 of pol III-transcribed genes. As a consequence of substantially different upstream promoters utilized by plant and human pol III, in vitro transcription of 7SL RNA genes in heterologous systems is severely impaired. A chimeric human 7SL RNA gene that contains the 5¢ flanking region up to position )300 of At7SL-1 is yet transcribed with a reduced efficiency in tobacco extract when compared with the plant wild-type gene, supporting the notion that internal regulatory elements contribute to full activity.

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Molecular analysis of the gene family of the signal recognition particle (SRP) RNA of tomato

Cited by 7 publications

References 54 publications

Expression of modified 7SL RNA gene in transgenic Solanum tuberosum plants

Expression of modified 7SL RNA gene in transgenic Solanum tuberosum plants

Exploring the sound-modulated delay in tomato ripening through expression analysis of coding and non-coding RNAs

Plant 7SL RNA genes belong to type 4 of RNA polymerase III‐ dependent genes that are composed of mixed promoters

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