Molecular analysis of the graviperception signal transduction in the flagellate Euglena gracilis: Involvement of a transient receptor potential-like channel and a calmodulin

Häder, Donat-Peter; Richter, Peter; Schuster, Martin; Daiker, Viktor; Lebert, Michael

doi:10.1016/j.asr.2009.01.029

Cited by 36 publications

(38 citation statements)

References 34 publications

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“…Our results are in strong agreement with other studies that reported higher sensitivity of gravitactic orientation in Euglena to various toxins (Ahmed and Häder 2010b;Tahedl and Häder 1999). E. gracilis has calcium-sensitive channels in the cell membrane acting as gravireceptors (Häder et al 2009) which can be a possible reason for the higher sensitivity of gravitactic orientation in Euglena to wastewaters and other toxins (Ahmed and Häder 2010b).…”

Section: Discussionsupporting

confidence: 94%

Sensitivity of various parameters in Euglena gracilis to short-term exposure to industrial wastewaters

Azizullah

Richter

Häder³

2011

J Appl Phycol

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Due to the presence of various potential pollutants industrial wastewaters pose considerable threats to natural waters and make it unfit for the aquatic biota. Analysis of wastewaters by chemical methods is a common practice; however, it does not reflect the toxic effects on living organism. Therefore, bioassessment is necessary for monitoring of wastewater quality. In the present study, the toxic effects of wastewater samples from different industries were evaluated using Euglena gracilis as a biotest organism. Various parameters of the freshwater flagellate E. gracilis like motility, swimming velocity, cell shape, gravitactic orientation (using the automatic biotest ECOTOX) and photosynthetic efficiency (using a pulse amplitude modulated fluorometer) were used as end points for toxicity assessment. In addition, the samples were analysed for some ecologically important physicochemical properties. With some exceptions, most of the physicochemical properties of the tested samples were within the acceptable range of national environmental quality standards for municipal and industrial effluents. However, all the water samples adversely affected different parameters in E. gracilis. This study led to the conclusion that different toxic substances present in wastewater, even at low concentrations, can be a possible threat to aquatic biota. The results of this study prove that ECOTOX is a sensitive, easy, and fast bioassay for monitoring of water and wastewater quality. Gravitactic orientation and cell compactness of E. gracilis were the most sensitive parameters to wastewater toxicity.

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Section: Discussionsupporting

confidence: 94%

Sensitivity of various parameters in Euglena gracilis to short-term exposure to industrial wastewaters

Azizullah

Richter

Häder³

2011

J Appl Phycol

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“…This is consistent with Tahedl and Häder (1999) reporting that orientation parameters respond more sensitively than motility to toxins. This may be explained by the presence of calcium-sensitive channels on the cell membrane which have a role as gravireceptors (Häder et al 2009). Shishida et al (2000) have tested the quality of different sewage treatment processes of wastewater by means of bioassays.…”

Section: Discussionmentioning

confidence: 98%

Monitoring of Waste Water Samples Using the ECOTOX Biosystem and the Flagellate Alga Euglena gracilis

Ahmed

Häder²

2010

Water Air Soil Pollut

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In this study, a fast bioassay using the ECOTOX system to evaluate biological safety of waste water samples from different sources was performed. This biological system works full automatically to test water quality. The system uses the image analysis of movement behavior of the flagellate Euglena gracilis as a model organism. The measured parameters are cell motility, velocity, orientation (r-value, upward swimming and alignment) as well as cell form (compactness). In most tested waste samples, precision of orientation (r-value) is inhibited at concentrations which showed less effect on the other parameters. Motility and compactness were noticed to be the least inhibited parameters in response to waste water samples. The results of waste treatment plants revealed the efficiency of the used purification system. Movement as well as orientation parameters in E. gracilis showed high sensitivity toward chlorine. The order of sensitivity was motility>velocity> r-value>upward swimming with EC 50 values of 0.69, 0.81, 0.85, and 1.78 mgL −1 , respectively. ECOTOX with its test organism (E. gracilis) provides an automatic, fast and sensitive system to monitor water samples.

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“…; Haeder et al . ). For higher plants, however, the precise mechanism of how gravity‐induced statolith movement is transduced into a physiological response in statocytes is – despite some progress – still enigmatic.…”

Section: Implementation Of the Recommendations From The 1996 Workhopmentioning

confidence: 97%

“…In an elegant sequence of flight experiments, the various components of the signal transduction chain and their (probable) sequence were determined (Haeder et al . , ; Daiker et al . ; Nasir et al .…”

Section: Implementation Of the Recommendations From The 1996 Workhopmentioning

confidence: 99%

Plant biology in space: recent accomplishments and recommendations for future research

Ruyters

Braun

2013

Plant Biol J

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Gravity has shaped the evolution of life since its origin. However, experiments in the absence of this overriding force, necessary to precisely analyse its role, e.g. for growth, development, and orientation of plants and single cells, only became possible with the advent of spaceflight. Consequently, this research has been supported especially by space agencies around the world for decades, mainly for two reasons: first, to enable fundamental research on gravity perception and transduction during growth and development of plants; and second, to successfully grow plants under microgravity conditions with the goal of establishing a bioregenerative life support system providing oxygen and food for astronauts in long-term exploratory missions. For the second time, the International Space Life Sciences Working Group (ISLSWG), comprised of space agencies with substantial life sciences programmes in the world, organised a workshop on plant biology research in space. The present contribution summarises the outcome of this workshop. In the first part, an analysis is undertaken, if and how the recommendations of the first workshop held in Bad Honnef, Germany, in 1996 have been implemented. A chapter summarising major scientific breakthroughs obtained in the last 15 years from plant research in space concludes this first part. In the second part, recommendations for future research in plant biology in space are put together that have been elaborated in the various discussion sessions during the workshop, as well as provided in written statements from the session chairs. The present paper clearly shows that plant biology in space has contributed significantly to progress in plant gravity perception, transduction and responses - processes also relevant for general plant biology, including agricultural aspects. In addition, the interplay between light and gravity effects has increasingly received attention. It also became evident that plants will play a major role as components of bioregenerative life support and energy systems that are necessary to complement physico-chemical systems in upcoming long-term exploratory missions. In order to achieve major progress in the future, however, standardised experimental conditions and more advanced analytical tools, such as state-of-the-art onboard analysis, are required.

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Molecular analysis of the graviperception signal transduction in the flagellate Euglena gracilis: Involvement of a transient receptor potential-like channel and a calmodulin

Cited by 36 publications

References 34 publications

Sensitivity of various parameters in Euglena gracilis to short-term exposure to industrial wastewaters

Sensitivity of various parameters in Euglena gracilis to short-term exposure to industrial wastewaters

Monitoring of Waste Water Samples Using the ECOTOX Biosystem and the Flagellate Alga Euglena gracilis

Plant biology in space: recent accomplishments and recommendations for future research

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