Molecular analysis of the rRNA genes of Babesia spp and Ehrlichia canis detected in dogs from Ribeirão Preto, Brazil

Oliveira, L.P.; Cardozo, G.P.; Santos, EV; Donini, I.A.N.; Zissou, V.G.; Roberto, Patrícia G.; Marins, Mozart

doi:10.1590/s1517-83822009000200006

Cited by 8 publications

(4 citation statements)

References 11 publications

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“…vogeli isolate in this study clustered in the same subclade as strains found in dogs from the states of São Paulo and Minas Gerais and from countries belonging to different continents, such as Venezuela, Nigeria, South Africa, Egypt and China (Matjila et al 2004, Passos et al 2005, Rey-Valeirón et al 2007, Sasaki et al 2007, Oliveira et al 2009, Xu et al 2015 (Fig.3).…”

Section: Discussionsupporting

confidence: 64%

Molecular epidemiology of Anaplasma platys, Ehrlichia canis and Babesia vogeli in stray dogs in Paraná, Brazil

et al. 2017

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Hemoparasitic infections are tick-borne diseases, which affect animals and humans. Considering the importance of canine hemoparasitic infections in veterinary clinics, this study aimed to determine the occurrence of Anaplasma platys, Ehrlichia canis and Babesia vogeli in blood samples from 182 dogs not domiciled in the city of Pato Branco, southwestern region of Paraná State, Brazil, using polymerase chain reaction (PCR). The prevalence of A. platys and B. vogeli was 32.9% and 10.9% respectively, and A. platys infection prevailed (p<0.001). The number of dogs positive for A. platys was larger in Winter (p<0.05). All blood samples were negative for E. canis. In the dogs, infestation by Amblyomma cajennense predominated over that by Rhipicephalus sanguineus (p<0.001); but there was no significant association between PCR and the variables presence of ticks, sex and age. Dogs infected by A. platys and B. vogeli showed thrombocytopenia, lymphopenia and leukocytosis; but there was no correlation between such hematological changes and infection by hemoparasites. This appears to be the first molecular study that demonstrates the existence of A. platys and B. vogeli in dogs from the southwestern region of Paraná.

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Section: Discussionsupporting

confidence: 64%

Molecular epidemiology of Anaplasma platys, Ehrlichia canis and Babesia vogeli in stray dogs in Paraná, Brazil

et al. 2017

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“…The first molecular detection of E. canis in Brazilian cats was reported in a study at a VTH of Minas Gerais State, Southeastern Brazil, in which blood samples from 3/15 cats tested positive by nested-PCR for the 16S rRNA gene. The E. canis sequence, a fragment of the 16S rRNA gene, showed 100% identity with the E. canis sequence obtained from dogs from the same study area (OLIVEIRA et al, 2009a(OLIVEIRA et al, , 2009b. Further studies are needed to better characterize the Ehrlichia spp.…”

Section: Ehrlichiosis In Catsmentioning

confidence: 92%

“…Ehrlichiosis is widely detected across Brazil (Figure 3). Three species have been described to date in Brazil: E. canis (AGUIAR et al, 2007a;LABRUNA et al, 2007;UENO et al, 2009;DINIZ et al, 2007;OLIVEIRA et al, 2009a), E. ewingii (OLIVEIRA et al, 2009c) and E. chaffeensis (MACHADO et al, 2006). This is a review of recent studies on the occurrence of Ehrlichia species in domestic and wild animals, ticks and humans in Brazil.…”

Section: Introductionmentioning

confidence: 99%

Ehrlichiosis in Brazil

Vieira

Biondo

Guimarães

et al. 2011

Rev. Bras. Parasitol. Vet.

123

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Ehrlichiosis is a disease caused by rickettsial organisms belonging to the genus Ehrlichia. In Brazil, molecular and serological studies have evaluated the occurrence of Ehrlichia species in dogs, cats, wild animals and humans. Ehrlichia canis is the main species found in dogs in Brazil, although E. ewingii infection has been recently suspected in five dogs. Ehrlichia chaffeensis DNA has been detected and characterized in mash deer, whereas E. muris and E. ruminantium have not yet been identified in Brazil. Canine monocytic ehrlichiosis caused by E. canis appears to be highly endemic in several regions of Brazil, however prevalence data are not available for several regions. Ehrlichia canis DNA also has been detected and molecularly characterized in three domestic cats, and antibodies against E. canis were detected in free-ranging Neotropical felids. There is serological evidence suggesting the occurrence of human ehrlichiosis in Brazil but its etiologic agent has not yet been established. Improved molecular diagnostic resources for laboratory testing will allow better identification and characterization of ehrlichial organisms associated with human ehrlichiosis in Brazil.

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“…A preliminary investigation of Ehrlichia species was developed in the northern and southeastern regions of Brazil, including various tick species, humans, dogs, and capybaras, but no Ehrlichia DNA was found in human samples (Labruna et al 2007). In contrast, ehrlichial DNA compatible with Ehrlichia chaffeensis and Ehrlichia ewingii were reported in animal blood samples in Brazil (Machado et al 2007, Oliveira et al 2009. A previous report from Pará State revealed an overall canine seroprevalence of 16.2% for E. canis (Spolidorio et al 2013) Brazilian spotted fever (BSF), a rickettsial disease caused by Rickettsia rickettsii, is the most important tick-borne zoonosis in Brazil, where the disease has been reported since 1929 (Piza et al 1932).…”

Section: Introductionmentioning

confidence: 99%

Seroprevalence of Tick-Borne Pathogens and Tick Infestation in Dogs from Tapirapé and Karajá Indigenous Communities, Brazil

Minervino

Lima

Soares

et al. 2015

Vector-Borne and Zoonotic Diseases

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With the aim of studying some tick-borne diseases, a total of 327 dogs (114 from Tapirapé and 213 from Karajá indigenous ethnicity, Mato Grosso, MT, Brazil) were sampled. Serum samples were submitted to the indirect fluorescence antibody test (IFAT) to detect antibodies against Babesia vogeli, Ehrlichia canis, and Rickettsia spp. Possible associations of risk factors and the occurrence of seroreativity to tick-borne agents and tick infestations were analyzed through chi-squared tests. Among 327 dogs, 46 (13.15%) were seropositive for B. vogeli and 47 (14.37%) for E. canis. The B. vogeli seroprevalence was higher for Karajá and for adult dogs (p>0.05). No association was found for E. canis seroprevalence. From 103 serum samples tested with rickettsial antigens, 90 (87%) dogs were seropositive to Rickettsia spp., with highest reactivity to Rickettsia amblyommii. Canine seropositivity to Rickettsia spp. was associated (p<0.05) with ethnicity (higher seroprevalence in Tapirapé dogs), age (higher in adults), and hunting (higher among hunting dogs). From the 327 dogs, 39 (11.9%) were infested by ticks (Amblyomma cajennense sensu stricto, Amblyomma ovale, Amblyomma oblongoguttatum, Amblyomma tigrinum, and Rhipicephalus sanguineus). Infestations by Amblyomma spp. ticks were higher in dogs from Tapirapé community and in hunting dogs (p<0.05). Regarding R. sanguineus, infestations were higher (p<0.05) among young dogs.

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Molecular analysis of the rRNA genes of Babesia spp and Ehrlichia canis detected in dogs from Ribeirão Preto, Brazil

Cited by 8 publications

References 11 publications

Molecular epidemiology of Anaplasma platys, Ehrlichia canis and Babesia vogeli in stray dogs in Paraná, Brazil

Molecular epidemiology of Anaplasma platys, Ehrlichia canis and Babesia vogeli in stray dogs in Paraná, Brazil

Ehrlichiosis in Brazil

Seroprevalence of Tick-Borne Pathogens and Tick Infestation in Dogs from Tapirapé and Karajá Indigenous Communities, Brazil

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