Molecular Analysis of the Triticale Lines with Different Vrn Gene Systems Using Microsatellite Markers and Hybridization In Situ

Abstract

Hexaploid triticale ( × Triticosecale Wittmack) lines were examined using molecular markers and the hybridization in situ technique. Triticale lines were generated based on wheat varieties differing by the Vrn gene systems and the earing times. Molecular analysis was performed using Xgwm and Xrms microsatellite markers with the known chromosomal localization in the common wheat Triticum aestivum , and rye Secale cereale genomes. Comparative molecular analysis of triticale lines and their parental forms showed that all lines contained A and B genomes of common wheat and also rye homoeologous chromosomes. In the three lines the presence of D genome markers, mapped to the chromosomes 2D and 7D, was demonstrated. This was probably the consequence of the translocations of homoeologous chromosomes from wheat genomes, which took part during the process of triticale formation. The data obtained by use of genomic in situ hybridization supported the data of molecular genetic analysis. In none of the lines wheat-rye translocations or recombinations were observed. These findings suggest that the change of the period between the seedling appearance and earing time in triticale lines compared to the initial wheat lines, resulted from the inhibitory effect of rye genome on wheat vernalization genes. PLANT GENETICS

Analysis of VRN1 gene in triticale and common wheat genetic background

“…Analyses of the triticale lines obtained by crossing wheat with different VRN genotypes and rye showed that heading time in triticale was later in comparison to the initial wheat forms and showed greater frost resistance (Khotyljova et al, 2002;Leonova et al, 2005). In our experiment all analyzed triticale accessions headed earlier than wheat, despite a VRN-1 genotype.…”

THE USE OF Vrn GENES FOR CREATION OF TRITICALE FORMS WITH DIFFERENT LENGTH OF VEGETATION PERIOD (review)

“…Rye has Vrn-R1 gene on the chromosome 5RL (J. Plaschke et al, 1993) Leonova et al, 2005). The genetic control of growth habit of spontaneous spring mutants is currently unknown.…”

“…При изучении этих линий обнаружено ингибирующее действие генетической среды тритикале на проявление доминантных генов Vrn: у тритикале растения выколашивались позднее, чем у соответствующих линий пшеницы. При этом доминантный ген Vrn-A1, определяющий раннее выколашивание, подавлялся в большей степени, чем Vrn-B1 и Vrn-D1 (22,82). Позднее в Сибирском НИИ растениеводства и селекции СО РАСХН были созданы октаплоидные линии тритикале с доминантными генами Vrn посредством скрещивания изогенных по генам Vrn-A1, Vrn-B1, Vrn-D1 и Vrn-D4 линий мягкой пшеницы Triple Dirk с озимой диплоидной рожью сорта Короткостебельная 69.…”

Application of Rye SSR Markers for Detection of Genetic Diversity in Triticale

“…It is a crop with broad genetic potential and is widely adapted to abiotic stress conditions such as aluminium toxicity, drought, salinity, acidic or waterlogged soils (Kuleung et al, 2004). Triticale is also resistant to different diseases of wheat (Leonova et al, 2005). Triticale constitutes a valuable genetic resource for transferring genes of interest from rye into wheat, particularly those related to biotic and abiotic stresses (Vaillancourt et al, 2007).…”