Molecular Analysis of Transgene (psy) Inheritance in a Golden Rice Line Developed in the Genetic Background of IR64

Analysis of transgene(s) (psy+crtI) inheritance and its stability over generations in the genetic background of indica rice cultivar Swarna

“…However the transgene in G1 event showed normal segregation in BC 1 F 1 when F 1 was female while generating BC 1 F 1 seeds. The transgene segregation distortion of G1 event in BC 1 F 2 may be due to differential transmission of transgene(s) via male gametes as earlier observed (Nandakumar et al 2008). In BC 1 F 1 , the same G1 event showed normal segregation when F 1 was used as female to produce BC 1 F 1 seed.…”

“…Transgene segregation distortion in rice has been reported earlier by several workers (Peng et al 1995;Chareonpornwattana et al 1999;Kim et al 1999;Ghakawa et al 2000;Vain et al 2002). The severe segregation distortion of psy in F 2 as well as in BC 1 F 1 population involving Swarna as recurrent parent was reported earlier (Nandakumar et al 2008). Segregation distortion of the transgene was explained due to its location in the genome, near the gametophytic selection locus ga2 and not because effect of transgene per se.…”

“…Segregation distortion of the transgene was explained due to its location in the genome, near the gametophytic selection locus ga2 and not because effect of transgene per se. Further in BC 2 F 2 , the transgene showed the normal segregation probably due to fixation of the ga2 locus in homozygous condition (Nandakumar et al 2008). However the transgene in G1 event showed normal segregation in BC 1 F 1 when F 1 was female while generating BC 1 F 1 seeds.…”

Transgenic Peanut (Arachis hypogaea L.) Overexpressing mtlD Gene Showed Improved Photosynthetic, Physio-Biochemical, and Yield-Parameters under Soil-Moisture Deficit Stress in Lysimeter System

“…The best performing T events, MTD1 and MTD4, will be used for open-field trials for their release as cultivars, especially for use in drought-prone areas. Furthermore, these identified T lines can also be used as a valuable pre-breeding resource to transfer the transgene using a backcross breeding approach into other favored genetic backgrounds, such as, backcross-derived Golden rice lines of Swarna (Nandakumar et al, 2008 ) and drought-tolerant TaDREB3 -derived lines of wheat (Shavrukov et al, 2016 ). The results clearly signified that mannitol accumulation in the T lines played an important role in osmoregulation and hydroxyl radical scavenging, which facilitated maintenance of better photosynthetic machinery and lower oxidative damage.…”

Epigenetics: History, present status and future perspective

“…Ever since the domestication of plants, considerable progress has been made in agriculture due to human behavioral changes from food gathering to farming. Domestication followed by selection of plants with desirable traits, breeding varieties for higher yield, tolerance to abiotic and biotic stresses, better quality and nutrition (Singh et al 2002;Nandakumar et al 2008;Parida et al 2009;Marathi et al 2012) and the technological advancements (Nandakumar et al 2004;Kumar et al 2006;Chikkappa et al 2011;Kumar 2014) for generating better agricultural inputs enabled more than four times increase in food grain production in India from 50 million tons in 1950 to 273 million tons in 2016 (Kulkarni 2017). While plant breeding aims at developing newer crop varieties with wider adaptation to the changing climatic condition, understanding the adaptation process in plants to the changing environmental conditions is also an interesting phenomenon.…”