Molecular analysis of VHI+ B lymphocytes in hepatitis C patients

Galli-Stampino, Luisa; Pasqualini, A.; Pozzato, Gabriele; Bonino, Ferruccio; Filipponi, Franco; Mosca, Marta; Masciopinto, Francesca; Abrignani, Sergio; Uematsu, Yasushi

doi:10.1016/s1590-8658(03)00452-3

Cited by 6 publications

(3 citation statements)

References 25 publications

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“…These observations support the notion of compartmentalization of lymphocyte subpopulations in the hepatic microenvironment in which activation, differentiation and proliferation of B cells occur [11–13,31]. Our group has demonstrated recently a disproportionate distribution of B cell clonotypes in different biological compartments.…”

Section: Discussionsupporting

confidence: 87%

Virological analysis and phenotypic characterization of peripheral blood lymphocytes of hepatitis C virus-infected patients with and without mixed cryoglobulinaemia

Sansonno

Lauletta

Montrone

et al. 2005

Clinical and Experimental Immunology

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Section: Discussionsupporting

confidence: 87%

Virological analysis and phenotypic characterization of peripheral blood lymphocytes of hepatitis C virus-infected patients with and without mixed cryoglobulinaemia

Sansonno

Lauletta

Montrone

et al. 2005

Clinical and Experimental Immunology

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“…B cell clonal expansion has been reported in small cohorts of HCV‐infected patients, more frequently in those who are mixed cryoglobulin positive (15–18). Clonal B cell proliferation has also been observed in liver biopsy specimens obtained from Italian patients with HCV infection (19–23). Another manifestation of the lymphoid tropism of HCV may be the development of B cell non‐Hodgkin's lymphoma (NHL).…”

mentioning

confidence: 99%

Clonal B cell populations in the blood and liver of patients with chronic hepatitis C virus infection

Vallat¹,

Benhamou²,

Gutierrez³

et al. 2004

Arthritis & Rheumatism

107

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Objective. The association of hepatitis C virus (HCV) infection with type II mixed cryoglobulinemia is well established, but the role of HCV in B cell lymphoma remains controversial. The objective of this study was to determine the frequency of circulating and liverinfiltrating monoclonal B cells in patients with HCV infection.Methods. One hundred sixty patients were studied prospectively, including 115 HCV-positive patients and 45 HCV-negative patients with other nonimmune chronic liver disease(s). B cell clonality was determined by DNA amplification of the IgH rearrangements, followed by polyacrylamide gel electrophoresis.Results. A clonal B cell population was detected in the blood of 21 (26%) of 81 HCV-positive patients whose cryoglobulin status was known, including 12 of 25 patients with type II cryoglobulinemia, 2 of 12 patients with type III cryoglobulinemia, and 7 of 44 patients without cryoglobulins. A clonal IgH rearrangement was detected in 26 (32%) of 81 liver biopsy specimens from HCV-infected patients, including 16 patients with a circulating clonal population. A clonal B cell population was not observed in the blood of 40 patients with non-HCV liver diseases and was present in only 1 (3%) of 30 liver biopsy specimens. Logistic regression analysis showed that HCV-infected patients with clonal B cell proliferation in both the blood and liver were older (P ‫؍‬ 0.004) and had longer duration of HCV infection (P ‫؍‬ 0.009), higher serum cryoglobulin levels (P ‫؍‬ 0.001) that were more frequently symptomatic (P < 0.03), and liver disease that was more severe than that in patients without a clonal B cell population in the blood or liver (P ‫؍‬ 0.05). In 4 of 16 patients with a clonal B cell population in both the blood and liver, a definite B cell malignancy was finally diagnosed.Conclusion. Clonal B lymphocytes are frequently detected in the blood and liver of patients with chronic HCV infection, in the absence of overt B cell malignancy. These clones are usually, but not always, associated with the presence of type II cryoglobulins. A high percentage of patients with B cell clonality in both the blood and liver were finally diagnosed as having a definite B cell malignancy.

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“…We hypothesized that the HCV viral load rebound beginning 1 day post LT would cause unspecific activation and an expansion of CD19+ B cells. Many reports have demonstrated that HCV has the capacity to proliferate inside CD19+ B cells, which can result in the unspecific proliferation of these cells (11) as well as apoptosis inhibition (12). It was precisely during this period shortly after transplantation when the most infections occurred in the HCV group.…”

Section: Discussionmentioning

confidence: 99%

High rate of infection and immune disorders in patients with hepatitis C virus after liver transplantation

Micheloud

Salcedo

Bañares

et al. 2009

Transplant Infectious Dis

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The aim of this prospective study was to analyze the incidence of serious infections and changes in immunological markers after liver transplantation (LT) in a cohort of patients with hepatitis C virus (HCV). This study included 34 patients who had LT, 20 patients with HCV etiology (HCV group), and 14 patients with alcoholic etiology (non-HCV group). Patients with HCV were more likely to have severe infections (80%) in comparison with patients in the non-HCV group (42%) (P=0.05). The HCV group had a 3-fold greater likelihood of early severe bacterial infections than the non-HCV group. At 1 week post LT, the HCV group showed higher values of CD19+ B cells/microL than the non-HCV group (P<0.05). At weeks 4 and 12 post LT, the HCV group had lower values of CD19+ B cells/microL (P<0.05). Our data suggest that HCV recurrence after LT was associated with a high incidence of early severe infections and immunological alterations, which may be related to this increased risk.

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Molecular analysis of VHI+ B lymphocytes in hepatitis C patients

Cited by 6 publications

References 25 publications

Virological analysis and phenotypic characterization of peripheral blood lymphocytes of hepatitis C virus-infected patients with and without mixed cryoglobulinaemia

Virological analysis and phenotypic characterization of peripheral blood lymphocytes of hepatitis C virus-infected patients with and without mixed cryoglobulinaemia

Clonal B cell populations in the blood and liver of patients with chronic hepatitis C virus infection

High rate of infection and immune disorders in patients with hepatitis C virus after liver transplantation

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