Molecular and agronomic evaluation of wheat doubled haploid lines obtained through maize pollination and anther culture methods

Abstract

Although maize pollination (MP) and anther culture (AC) are alternative techniques widely used for wheat doubled haploid (DH) production, there is only limited information on the attributes of the plant materials produced through both methods. This study was conducted to evaluate genetic fidelity, transmission of parental gametes, and to compare field performance of DH populations produced by the MP and AC methods from the F1s of two crosses between spring bread wheat cultivars. The DH populations were compared to single seed descent (SSD) lines created from the same crosses. In total, 76 MP and 122 AC lines of the cross between cultivars of divergent origin were subjected to RAPD and AFLP analysis. Only changes in AFLP banding patterns, at similarly low frequencies, 0.18% (MP) and 0.21% (AC), were detected. The frequency of the DH lines affected by the variation, 14.5% (MP) and 14.8% (AC), was similar in both populations. For most of the DH lines, variation in 1‐2 loci only, out of several hundreds scored, was observed. A total of 14.3% (MP) and 22.2% (AC) marker loci showed the significant segregation distortion from the expected 1 : 1 ratio, but in at least one polymorphic locus the within‐cultivar variation was responsible for the skewed segregation. The field performance of the corresponding MP and AC lines derived from two crosses confirmed the equivalency of both DH populations. In most of the traits analyzed, the MP and AC lines performed the same as the SSD populations created from the same crosses. No, or very small differences in means and ranges, were observed when the best 10% of the lines from all three methods were compared. Moreover, the best 10 % of the lines of the cross between Polish wheat cultivars adapted to the local environment performed significantly better for some traits than different groups of checks used in the study.

In VitroResponse of Pepper Anther Culture (CapsicumannuumL.) Depending on Genotype, Nutrient Medium and Duration of Cultivation

“…in this manner by doubling chromosomes in haploid plants it is possible to obtain genetically stable homozygous lines in a short time thereby providing a method for speeding up and increasing the selection efficiency. Such homozygous DH plants have been used in breeding programmes for production of pure lines and new highly adaptive and productive varieties (1,4,8,15,16,18,20,21,23,24,35,41,48). A number of studies (2,19,37,45,53,55) also reported that the obtained by the in vitro anther culture techniques haploids and Dh plants are an appropriate system for genetic manipulations, gene mapping and mutant line selection.…”

Analysis of Yield and Yield Related Traits Variability of Winter Wheat (Triticum aestivum L.) Cv. Izolda and Double Haploid Lines

“…Literature data shows that wheat DH lines (MP and AC lines) differs in grain yield and some other yield parameters -yield-forming factors (Inagaki et al, 1998;GuzyWróbelska and Szarejko, 2003;Laurie and Snape, 1990). The variation in grain yield between DH lines and parent cultivars could be related to the origin of AC lines (DH androgenic genotypes) as well as MP lines derived from hybrids of F1, and parental cultivars used to hybridization in these experiments (Ma et al, 1999;Guzy-Wróbelska and Szarejko, 2003).…”

“…AC lines compared with MP lines, derived from wheat with maize crosses, yielded lower (Ma et al, 1999) or higher (Guzy-Wróbelska and Szarejko, 2003). Literature data shows that wheat DH lines (MP and AC lines) differs in grain yield and some other yield parameters -yield-forming factors (Inagaki et al, 1998;GuzyWróbelska and Szarejko, 2003;Laurie and Snape, 1990).…”

“…According to literature, winter wheat DH lines derived from wheat anther culture (AC lines) yielded lower or higher in relation to parents used to hybridization (Ma et al, 1999;Guzy-Wróbelska and Szarejko, 2003). AC lines compared with MP lines, derived from wheat with maize crosses, yielded lower (Ma et al, 1999) or higher (Guzy-Wróbelska and Szarejko, 2003).…”

“…In wheat, two techniques are frequently used for doubled haploid production: anther culture (AC) and wheat with maize hybridization (MP) (Ma et al, 1999;Guzy-Wróbelska and Szarejko, 2003). Production techniques of wheat doubled haploids (DH) are worldwide reported (Zheng, 2003;Konieczny et al, 2003;Segui-Simarro and Nuez, 2008;Cistué et al, 2009;Redha and Islam, 2010;Germanà, 2011;Islam and Tuteja, 2012).…”