Molecular and bacteriological investigations for the co-existence CRISPR/Cas system and β-lactamases of types extended-spectrum and carbapenemases in Multidrug, extensive drug and Pandrug-Resistant Klebsiella pneumoniae

The clustered regularly interspaced short palindromic repeat (CRISPR)-Caspase (Cas) system acts as a natural defense of bacteria against invasion by mobile genetic elements, such as plasmids, transposons, and bacteriophages. The review discusses the different classes and types of CRISPR-Cas systems in terms of principles of their action, limitations, and future perspectives. Also, the role of the CRISPR-Cas system as a new arsenal in fighting multidrug-resistant pathogens and clinically relevant pathogenic viruses and up-to-date clinical trials have been discussed and highlighted. Moreover, the utilization mode, regulation, and the link of CRISPR-Cas to quorum sensing for targeting cell chromosome or antimicrobial-resistant gene(s) of some clinically relevant pathogens, including Klebsiella pneumoniae, Escherichia coli, and Pseudomonas aeruginosa have been discussed. The information provided in this review can act as a platform for researchers for the future use of the CRISPR-Cas system as a smart antimicrobial agent for combating infections caused by life-threatening pathogens, particularly those with limited therapeutic options.

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An investigation of carbapenemase-encoding genes inBurkholderia cepaciaandAeromonas sobrianosocomial infections among Iraqi patients

Al-Ouqaili,

Hussein,

Kanaan

et al. 2024

Preprint

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Burkholderia cepacia and Aeromonas sobria are difficult to eradicate due to their innate resistance to a variety of medications, and cause various diseases. The aim of this study was to investigate the occurrence of carbapenemase genes and patterns of antibiotic resistance in isolates of B. cepacia and A. sobria. A cross-sectional study was conducted in the Ramadi Teaching Hospitals in the Al-Anbar Governorate in 2024. Various study samples, were used to collect the studied bacteria. The antibiotic resistance was detected by the VITEK®2 System. The presence of carbapenemase genes was confirmed via PCR technique. In this investigation, seventy-five (75) isolates of A. sobria and B. cepacia were assessed. Of these, A. sobria made up 16.6% (n = 20) while B. cepacia accounted for 45.8% (n = 55). The study isolates showed highest antimicrobial resistance to piperacillin, cefepime, ceftriaxone (100%), ceftazidime (97.3%), and lowest antimicrobial resistance to imipenem (36%). The result showed 55/57 recA gene positive for differentiated B. cepacia complex from other Burkholderia spp. The overall prevalence of carbapenemase genes was 92.8%% (52/56) with blaKPC accounting for 80.8% (42/52) and blaGES for 19.2% (10/52) of the total. The 42 B. cepacia isolates that tested positive for carbapenem resistance were constituted of 38 blaKPC (n = 38) and two blaGES (n = 2); in contrast, four blaKPC (n = 4) and eight blaGES (n = 8) were present in the A. sobria isolates that tested positive for carbapenems resistance. None of isolates studied tested positive for the blaIMP gene. The recent study concluded that recA gene identification was more sensitive and specific technique for detection B. cepacia complex isolates. There was a notable predominance of blaKPC and blaGES carbapenemase producers among the isolates under investigation. The blaIMP gene was not found in any of the research isolates.

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Molecular and bacteriological investigations for the co-existence CRISPR/Cas system and β-lactamases of types extended-spectrum and carbapenemases in Multidrug, extensive drug and Pandrug-Resistant Klebsiella pneumoniae

Cited by 6 publications

References 41 publications

Transmission chains and molecular characterizations of extended-spectrum β-lactamase producing Enterobacteriaceae at a veterinary hospital in Chengdu, China

Transmission chains and molecular characterizations of extended-spectrum β-lactamase producing Enterobacteriaceae at a veterinary hospital in Chengdu, China

Role of CRISPR-Cas System as a New Approach in Fighting the Antimicrobial Resistance of Bacterial and Viral Pathogens

An investigation of carbapenemase-encoding genes inBurkholderia cepaciaandAeromonas sobrianosocomial infections among Iraqi patients

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