Molecular and biochemical biomarkers responses in the mussel Mytilus edulis collected from Southern Brazil coast

Rola, Regina Coimbra; Monteiro, Maurício da Costa; Reis, Sérgio Renan da Silva; Sandrini, Juliana Zomer

doi:10.1016/j.marpolbul.2012.01.004

Cited by 37 publications

(12 citation statements)

References 31 publications

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“…GST-omega class also has been identified of up-regulated genes in oysters exposed to hydrocarbon (Boutet et al, 2004b;Lüchmann et al, 2014), domestic sewage (Medeiros et al, 2008a) and in transplanted zebra mussels (Châtel et al, 2015). This GST isoform is also suggested to be involved in the antioxidant defense system (Medeiros et al, 2008a;Rola et al, 2012), that can be important during PAH exposures.…”

Section: Discussionmentioning

confidence: 96%

Exposure to phenanthrene and depuration: Changes on gene transcription, enzymatic activity and lipid peroxidation in gill of scallops Nodipecten nodosus

et al. 2016

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Understanding the mechanism of phenanthrene (PHE) biotransformation and related cellular responses in bivalves can be an important tool to elucidate the risks of polycyclic aromatic hydrocarbons (PAHs) to aquatic organisms. In the present study it was analyzed the transcriptional levels of 13 biotransformation genes related to cytochrome P450 (CYP), glutathione S-transferase (GST), sulfotransferase (SULT), flavin-containing monooxygenase and fatty acid-binding proteins by qPCR in gill of scallops Nodipecten nodosus exposed for 24 or 96h to 50 or 200μgL(-1) PHE (equivalent to 0.28 and 1.12μM, respectively), followed by depuration in clean water for 96h (DEP). Likewise, it was quantified the activity of catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD), glutathione reductase (GR), glucose 6-phosphate dehydrogenase (G6PDH), GST and levels of lipid peroxidation. Increased transcriptional levels of CYP2UI-like, CYP2D20-like, CYP3A11-like, GSTomega-like, SULT1B1-like genes were detected in organisms exposed to PHE for 24 or 96h. In parallel, GR and GPX activities increased after 96h exposure to 200μgL(-1) PHE and G6PDH activity increased after 24h exposure to 50μgL(-1) PHE. This enhancement of antioxidant and phase I and II biotransformation systems may be related to the 2.7 and 12.5 fold increases in PHE bioaccumulation after 96h exposure to 50 and 200μgL(-1) PHE, respectively. Interestingly, DEP caused reestablishment of GPX and GR activity, as well as to the transcript levels of all upregulated biotransformation genes (except for SULT1B1-like). Bioaccumulated PHE levels decreased 2.5-2.9 fold after depuration, although some biochemical and molecular modifications were still present. Lipid peroxidation levels remained lower in animals exposed to 200μgL(-1) PHE for 24h and DEP. These data indicate that N. nodosus is able to induce an antioxidant and biotransformation-related response to PHE exposure, counteracting its toxicity, and DEP can be an effective protocol for bivalve depuration after PHE exposure.

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Section: Discussionmentioning

confidence: 96%

Exposure to phenanthrene and depuration: Changes on gene transcription, enzymatic activity and lipid peroxidation in gill of scallops Nodipecten nodosus

et al. 2016

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“…Patos Lagoon in Brazil had lower CAT activity (in muscle), higher SOD activity (mantle) and expression (gills and muscle), higher GST activity (mantle) and expression (gills), and higher HSP70 expression (gills and muscle) than those mussels collected from a reference station (Rola et al, 2012). A study with mud snails (Bullacta exarata) in Bohai Bay, China demonstrated an increase in SOD activity and MT concentration, but no effect on GST and CAT activity and vitellin-like protein levels in areas where metal and PAH contamination were higher than the reference station flushing of a contaminated lake, exposed Manila clams (Ruditapes philippinarium) to resusupended sediment from the area.…”

Section: Mussels (Mytilus Edulis) Collected Near the Pollutedmentioning

confidence: 84%

Effects of Pollution on Marine Organisms

Mearns¹,

Reish²,

Oshida³

et al. 2013

Water Environment Research

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This review covers selected 2012 articles on the biological effects of pollutants and human physical disturbances on marine and estuarine plants, animals, ecosystems and habitats. The review, based largely on journal articles, covers field and laboratory measurement activities (bioaccumulation of contaminants, field assessment surveys, toxicity testing and biomarkers) as well as pollution issues of current interest including endocrine disrupters, emerging contaminants, wastewater discharges, dredging and disposal, etc. Special emphasis is placed on effects of oil spills and marine debris due in part to the 2010 Deepwater Horizon oil blowout in the Gulf of Mexico and the 2011 Japanese tsunami. Several topical areas reviewed in the past (ballast water and ocean acidification) were dropped this year. The focus of this review is on effects, not pollutant fate and transport. There is considerable overlap across subject areas (e.g. some bioaccumulation papers may be cited in other topical categories). Please use keyword searching of the text to locate related but distributed papers. Use this review only as a guide and please consult the original papers before citing them.

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Section: Polycyclicmentioning

confidence: 99%

“…Chemical analyses of environmental samples are difficult, expensive and timeconsuming method [6][7][8]. Analytical technique is unable to provide the risk assessment of contaminants and biological effects of xenobiotics after entering an ecosystem [5,7]. Biomarkers have been explained as biological assay including as molecular, biochemical, cellular and physiological responses that can be determined in different parts of organisms such fluids, cells and tissues [7][8][9].…”

Section: Polycyclicmentioning

confidence: 99%

Effects of Beta-Naphthoflavone and 3-Methylcholantheren on Biochemical Markers in Sturgeon Fish, Huso huso

Karimzadeh

2016

IJT

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Background: Polycyclic aromatic hydrocarbons (PAHs) are a predominant group of contaminants that have been shown to accumulate and persist in marine organisms. We evaluated the dose-dependent induction of the cytochrome P4501A (CYP1A) system in the Sturgeon fish, Huso huso after after exposure to PAHs, beta-naphthoflavone (BNF) and 3methylcholantheren. Methods: The fish were treated by i.p. injections of beta-naphthoflavone and 3methylcholantheren dissolved in corn oil at three doses 35, 70 and 105 mg/kg wet-body weight respectively for 72 h every day. Similarly, the control fish received injections of only corn oil. Microsomal fraction was prepared and ethoxy resorufin-O-deethylase activity (EROD) was measured in the fish liver. Cytochrome P4501A1 (CYP1A1) content was estimated by ELISA based on monoclonal anti-cod P4501A1. Results: Beta-naphthoflovone and 3-methylcholantheren treated-fish developed 15-32 fold increase in Ethoxyresorufin-O-deethylase activity in liver microsomes. The results of ELISA showed higher levels of cytochrome P4501A content in treated fish as compared to controls. Conclusion: A parallel increase of the CYP1A immunochemically assay and an increase in EROD activity could be recorded for beta-naphthoflavone and 3-methylcholantheren in injected fish. Dose-response relationships on the induction of EROD and CYP1A immunodetectable protein were recorded. Therefore, EROD activity and CYP1A content can be used as a biomarker of polycyclic aromatic hydrocarbons.

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Molecular and biochemical biomarkers responses in the mussel Mytilus edulis collected from Southern Brazil coast

Cited by 37 publications

References 31 publications

Exposure to phenanthrene and depuration: Changes on gene transcription, enzymatic activity and lipid peroxidation in gill of scallops Nodipecten nodosus

Exposure to phenanthrene and depuration: Changes on gene transcription, enzymatic activity and lipid peroxidation in gill of scallops Nodipecten nodosus

Effects of Pollution on Marine Organisms

Effects of Beta-Naphthoflavone and 3-Methylcholantheren on Biochemical Markers in Sturgeon Fish, Huso huso

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