Molecular and biochemical characterization of torenia flavonoid 3′-hydroxylase and flavone synthase II and modification of flower color by modulating the expression of these genes

Ueyama, Yukiko; Suzuki, Ken-ichi; Fukuchi-Mizutani, Masako; Fukui, Yûkô; Miyazaki, Kiyoshi; Ohkawa, Hideo; Kusumi, Takenori; Tanaka, Yoshikazu

doi:10.1016/s0168-9452(02)00098-5

Cited by 106 publications

(92 citation statements)

References 28 publications

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“…Flavone composition also changed; the level of luteolin decreased and that of apigenin increased, indicating that the F3ЈH gene is suppressed in SWV transgenic plants and that F3ЈH determines the hydroxylation pattern of the flavone Bring in SWV. This result is consistent with our previous study that the torenia F3ЈH can catalyze hydroxylation of apigenin to luteolin in vitro (Ueyama et al 2002). Suppression of the F3ЈH and F3Ј5ЈH transcripts and expression of rose or pelargonium DFR gene in SWV transgenic plants were confirmed by RT-PCR analysis (Figure 4, E-H).…”

Section: Cloning Pelargonium Dfr Cdna and Binary Vector Constructionsupporting

confidence: 92%

“…Pelargonium DFR cDNA was obtained by screening a cDNA library derived from Pelargonium zonale (L.) petals with rose DFR cDNA (Tanaka et al 1995). Torenia F3ЈH (Ueyama et al 2002) and F3Ј5ЈH (Suzuki et al 2000) cDNAs were used to downregulate their expression. The F3ЈH double stranded RNA, F3Ј5ЈH double stranded RNA, and the DFR genes were transcribed by constitutive promoters; the El235S promoter, which is an enhanced cauliflower mosaic virus 35S promoter from pBE2113-GUS (Mitsuhara et al 1996) or the Mac1 promoter (Comai et al 1990).…”

Section: Molecular Cloning and Binary Vector Construction For Flower mentioning

confidence: 99%

“…2000) and magenta flowers have been produced by additional overexpression of the torenia F3ЈH gene (Ueyama et al 2002). These results suggest that accumulation of pelargonidin-based anthocyanins is necessary to produce a more deep red or intense pink petal color.…”

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confidence: 91%

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Generation of pink flower varieties from blue Torenia hybrida by redirecting the flavonoid biosynthetic pathway from delphinidin to pelargonidin

Nakamura

Fukuchi-Mizutani

Fukui

et al. 2010

Plant Biotechnology

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Section: Cloning Pelargonium Dfr Cdna and Binary Vector Constructionsupporting

confidence: 92%

Section: Molecular Cloning and Binary Vector Construction For Flower mentioning

confidence: 99%

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Generation of pink flower varieties from blue Torenia hybrida by redirecting the flavonoid biosynthetic pathway from delphinidin to pelargonidin

Nakamura

Fukuchi-Mizutani

Fukui

et al. 2010

Plant Biotechnology

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“…Furthermore, ORF10 and ORF12 could be responsible for the introduction of the methoxyl group onto the indole ring. ORF12 shows sequence similarity to cytochrome P450 enzymes which catalyse the hydroxylation of flavonoids (Ueyama et al, 2002), while ORF10 is a homologue of methyltransferases which catalyse the methylation of a phenolic hydroxyl group (Dickens et al, 1995). By sequence analysis, however, it is not possible to assign the prenyltransferases FtmPT1 and FtmPT2 to the corresponding reactions.…”

Section: Resultsmentioning

confidence: 99%

Overproduction, purification and characterization of FtmPT1, a brevianamide F prenyltransferase from Aspergillus fumigatus

2005

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A putative prenyltransferase gene, ftmPT1, was identified in the genome sequence of Aspergillus fumigatus. ftmPT1 was cloned and expressed in Escherichia coli, and the protein FtmPT1 was purified to near homogeneity and characterized biochemically. This enzyme was found to catalyse the prenylation of cyclo-L-Trp-L-Pro (brevianamide F) at the C-2 position of the indole nucleus. FtmPT1 is a soluble monomeric protein, which does not contain the usual prenyl diphosphate binding site (N/D)DXXD found in most prenyltransferases, and which does not require divalent metal ions for its enzymic activity. K m values for brevianamide F and dimethylallyl diphosphate were determined as 55 and 74 mM, respectively. The turnover number was 5?57 s "1 . FtmPT1 showed a high substrate specificity towards dimethylallyl diphosphate, but accepted different tryptophan-containing cyclic dipeptides. Together with dimethylallyltryptophan synthase of ergot alkaloid biosynthesis, FtmPT1 belongs to a new group of prenyltransferases with aromatic substrates. INTRODUCTIONTrans-prenyltransferases (Bohlmann et al., 1998;Liang et al., 2002;Sacchettini & Poulter, 1997) are involved in the biosynthesis of terpenoids from C-5 units. These enzymes usually contain one or more prenyl diphosphate binding motifs, (N/D)DXXD, in their sequences (Bohlmann et al., 1998;Liang et al., 2002;Sacchettini & Poulter, 1997). Their enzymic reaction depends strictly on the presence of metal ions such as Mg 2+ or Mn 2+ (Bohlmann et al., 1998;Liang et al., 2002). A special group of prenyltransferases catalyses the attachment of a prenyl moiety to an aromatic nucleus. These enzymes show similar sequence motifs and metal ion requirements to those of the trans-prenyltransferases. Examples of this group are the prenyltransferases involved in the biosynthesis of the primary metabolites ubiquinone (Turunen et al., 2004), menaquinone (Suvarna et al., 1998), tocopherol (Schledz et al., 2001) and plastoquinone (Collakova & DellaPenna, 2001), and the prenyltransferase involved in the formation of the plant secondary metabolite shikonin (Yazaki et al., 2002). All these enzymes are membrane-bound proteins.In contrast, the dimethylallyltryptophan synthase (DMATS) that catalyses the prenylation of tryptophan at position C-4 of the indole nucleus during ergot alkaloid biosynthesis in the fungus Claviceps has been found to be a soluble protein and is active in a metal-free buffer containing EDTA (Cress et al., 1981;Lee et al., 1976;Tsai et al., 1995; Tudzynski et al., 1999). Recently, two further soluble prenyltransferases with aromatic substrates, which are active in the absence of metal ions and contain no (N/D)DXXD motifs, CloQ involved in the biosynthesis of clorobiocin from Streptomyces roseochromogenes (Pojer et al., 2003) and LtxC involved in the biosynthesis of lyngbyatoxins from Lyngbya majuscula (Edwards & Gerwick 2004), have been identified. Very recently, we identified an orthologue of DMATS, FgaPT2, in the genome sequence of Aspergillus fumigatus (Unsöld & Li, 2005). Inte...

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“…A pink variety was obtained by suppressing the flavonoid 3Ј,5Ј-hydroxylase (F3Ј,5ЈH) gene, and a yellow variety was obtained from a cultivar that contained both carotenoids and anthocyanins by suppression of CHS or DFR genes. Ueyama et al (2002) reported that a 'Summerwave' whose F3Ј,5ЈH expression had been suppressed was further transformed with the flavonoid 3Ј-hydroxylase (F3ЈH) sense gene. Some of the transgenic plants had an elevated amount of cyanidin-type anthocyanins and exhibited redder flower color.…”

Section: Modification Of Flower Colormentioning

confidence: 99%

Torenia fournieri (torenia) as a model plant for transgenic studies

Aida

2008

Plant Biotechnology

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Molecular and biochemical characterization of torenia flavonoid 3′-hydroxylase and flavone synthase II and modification of flower color by modulating the expression of these genes

Cited by 106 publications

References 28 publications

Generation of pink flower varieties from blue Torenia hybrida by redirecting the flavonoid biosynthetic pathway from delphinidin to pelargonidin

Generation of pink flower varieties from blue Torenia hybrida by redirecting the flavonoid biosynthetic pathway from delphinidin to pelargonidin

Overproduction, purification and characterization of FtmPT1, a brevianamide F prenyltransferase from Aspergillus fumigatus

Torenia fournieri (torenia) as a model plant for transgenic studies

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