Molecular and Biochemical Characterization of a Novel Class A β-Lactamase (HER-1) from
            <i>Escherichia hermannii</i>

Beauchef-Havard, Anne; Arlet, Guillaume; Gautier, Valérie; Labia, Roger; Grimont, Patrick A. D.; Philippon, A.

doi:10.1128/aac.47.8.2669-2673.2003

Cited by 16 publications

(9 citation statements)

References 28 publications

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“…The in vitro susceptibilities of these isolates showed that piperacillin-tazobactam, ceftazidime, cefazolin, cefixime, aztreonam, gentamicin, tobramycin imipenem, meropenem, and amikacin were active in vitro . Isolates also showed a low-level resistance against amoxicillin, similar to that reported by Fitoussi et al and Beauchef-Havard et al ( 9 , 10 ). However, more clinical experiments are mandatory.…”

Section: Discussionsupporting

confidence: 87%

Pyelonephritis Caused Solely by Escherichia hermanii

Tong

Bing

Sun

2014

Jundishapur J Microbiol

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Introduction:In contrast with Escherichia coli, the association of E. hermanii with urinary tract infections has not been described.Case Presentation:In this case, E. hermanii was the sole isolate recovered from urine specimens of a pyelonephritis patient. The organism was found to be susceptible to piperacillin-tazobactam, ceftazidime, cefazolin, cefixime, aztreonam, gentamicin, tobramycin, imipenem, meropenem and amikacin, and resistant to amoxicillin. Antibiotic treatment was initiated with oral cefixime (400 mg every 24 hours). The symptoms were relieved within 72 hours after therapy. A urine sample was taken seven days after antibiotic therapy. E. hermanii was no longer isolated.Discussion:The present case demonstrates that the uropathogenic E. hermanii clone can cause destruction of the kidneys. During asymptomatic bacteriuria or cystitis, the bacteria remain in the urinary tract. Even when pyelonephritis develops, inflammatory response of the host is still restricted to the urinary tract. These signs mean that uropathogenic E. hermanii may be not very virulent.

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Section: Discussionsupporting

confidence: 87%

Pyelonephritis Caused Solely by Escherichia hermanii

Tong

Bing

Sun

2014

Jundishapur J Microbiol

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“…However, biofilm formation and its potential role in infections with E. hermannii still have to be confirmed in other studies. Furthermore, E. hermannii has a natural resistance to penicillin, ampicillin, and carbenicillin because of its β-lactamase production - which complicates treatment [ 10 ]. Currently, there is insufficient clinical data on the optimal antibiotic treatment - but mostly various β-lactams and quinolones with in vivo susceptibility have been used.…”

Section: Resultsmentioning

confidence: 99%

Catheter Related Escherichia hermannii Sepsis in a Haemodialysis Patient

Rank¹,

Kristensen²,

Hansen³

et al. 2016

TOMICROJ

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Escherichia hermannii is an extremely rare etiological agent of invasive infection, and thus, the bacterium was initially considered non-pathogenic. However, in five previously reported case reports E. hermannii has been implicated as the sole pathogen. Our case report describes blood stream infection with E. hermannii in a haemodialysis patient with persisting symptoms, high fever and inflammatory markers despite appropriate antibiotic treatment until replacement of the dialysis catheter. We suspect biofilm formation to be a crucial pathogenic feature for E. hermannii in the maintenance of an infection, which stresses the necessity of antibiotic treatment along with catheter replacement in bloodstream- and catheter-related infection with E. hermannii.

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“…Both strains BP-1(T) and BP-2 are multidrug resistant Enterobacteriaceae which harbor extended spectrum β-lactamases and are therefore classified as a serious antimicrobial threat by the CDC (CDC, 2013 ; Iredell et al, 2016 ). The Class A β-lactamase identified by Resfams had high identity to bla HER family genes, which were previously described in Atlantibacter hermanii but only conferred resistance to penicillins (Beauchef-Havard et al, 2003 ). As this gene was proximal to a tetR response regulator, it is possible that it may be inducible, like the ampR/ampC system widespread in Enterobacter and Citrobacter (Vadlamani et al, 2015 ).…”

Section: Discussionmentioning

confidence: 81%

Superficieibacter electus gen. nov., sp. nov., an Extended-Spectrum β-Lactamase Possessing Member of the Enterobacteriaceae Family, Isolated From Intensive Care Unit Surfaces

et al. 2018

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Two Gram-negative bacilli strains, designated BP-1(T) and BP-2, were recovered from two different Intensive Care Unit surfaces during a longitudinal survey in Pakistan. Both strains were unidentified using the bioMerieux VITEK MS IVD v2.3.3 and Bruker BioTyper MALDI-TOF mass spectrometry platforms. To more precisely determine the taxonomic identity of BP-1(T) and BP-2, we employed a biochemical and phylogenomic approach. The 16S rRNA gene sequence of strain BP-1(T) had the highest identity to Citrobacter farmeri CDC 2991-81(T) (98.63%) Citrobacter amalonaticus CECT 863(T) (98.56%), Citrobacter sedlakii NBRC 105722(T) (97.74%) and Citrobacter rodentium NBRC 105723(T) (97.74%). The biochemical utilization scheme of BP-1(T) using the Analytic Profile Index for Enterobacteriaceae (API20E) indicated its enzymatic functions are unique within the Enterobacteriaceae but most closely resemble Kluyvera spp., Enterobacter cloacae and Citrobacter koseri/farmeri. Phylogenomic analysis of the shared genes between BP-1(T), BP-2 and type strains from Kluyvera, Citrobacter, Escherichia, Salmonella, Kosakonia, Siccibacter and Shigella indicate that BP-1(T) and BP-2 isolates form a distinct branch from these genera. Average Nucleotide Identity analysis indicates that BP-1(T) and BP-2 are the same species. The biochemical and phylogenomic analysis indicate strains BP-1(T) and BP-2 represent a novel species from a new genus within the Enterobacteriaceae family, for which the name Superficieibacter electus gen. nov., sp. nov., is proposed. The type strain is BP-1(T) (= ATCC BAA-2937, = NBRC 113412).

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Molecular and Biochemical Characterization of a Novel Class A β-Lactamase (HER-1) from Escherichia hermannii

Cited by 16 publications

References 28 publications

Pyelonephritis Caused Solely by Escherichia hermanii

Pyelonephritis Caused Solely by Escherichia hermanii

Catheter Related Escherichia hermannii Sepsis in a Haemodialysis Patient

Superficieibacter electus gen. nov., sp. nov., an Extended-Spectrum β-Lactamase Possessing Member of the Enterobacteriaceae Family, Isolated From Intensive Care Unit Surfaces

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