Molecular and biochemical characterizations of three fructose-1,6-bisphosphate aldolases from Clonorchis sinensis

Li, Shan; Bian, Meng; Wang, Xiaoyun; Chen, Xueqing; Xie, Zhizhi; Sun, Hengchang; Jia, Feifei; Liang, Pei; Zhou, Chenhui; He, Lei; Mao, Qiang; Huang, Bo; Liang, Chi; Wu, Zhongdao; Li, Xuerong; Xu, Jin; Yu, Xinbing

doi:10.1016/j.molbiopara.2014.04.005

Cited by 16 publications

(14 citation statements)

References 42 publications

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“…Enzymes associated with the glycolytic pathway that is used to generate energy in nematodes play important roles in parasite survival in the host. FBPA is an essential enzyme in energy production by carbohydrate metabolism and has been identified and characterized in some parasites (El-Dabaa et al, 1998; Starnes et al, 2009; Li et al, 2014). Beyond its normal glycolytic role, several studies have shown that it plays important roles in host invasion (Hu et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…Class-I enzymes are mainly expressed in animals and plants, while Class-II enzymes are found in bacteria and lower eukaryotes (Maurady et al, 2002). FBPA has been identified in many parasites and plays an essential role during the development and survival of helminths, is involved in nutrient transport through the tegument, egg laying and muscular activity due to its involvement in carbohydrate metabolism (Lorenzatto et al, 2012; Li et al, 2014; Hu et al, 2015). FBPA not only contributes to energy generation in the glycolytic pathway but also has non-glycolytic effects on various key processes, for instance, adhesion to host cells, plasminogen binding, invasion, and immune evasion (Starnes et al, 2009; Lorenzatto et al, 2012; Hu et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Molecular Characterization of Fructose-1,6-bisphosphate Aldolase From Trichinella spiralis and Its Potential in Inducing Immune Protection

Yang

Bai

et al. 2019

Front. Cell. Infect. Microbiol.

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Trichinella spiralis is a major food-borne parasite worldwide. Trichinellosis caused by T. spiralis is not only a public health problem, but also an economic hazard in food safety. The development of effective vaccines to prevent Trichinella infection in domestic animals and humans is urgently needed for controlling of this zoonosis. Fructose-1, 6-bisphosphate aldolase (FBPA) is involved in energy production in glycolysis and is also associated with many non-glycolysis functions in the parasite, such as adhesion to host cells, plasminogen binding, and invasion. FBPA has been considered as a potential vaccine candidate or as a target for chemotherapeutic treatment. Here, we report for the first time the characterization of FBPA of T. spiralis and an evaluation of its potential as a vaccine candidate antigen against T. spiralis infection in mice. The results of qPCR and western blot analysis showed that the Ts -FBPA gene was expressed at various developmental stages of T. spiralis and was also detected in excretory–secretory products (ES) of T. spiralis muscle larvae (ML). Immunostaining with anti- Ts -FBPA mouse sera indicated that it localized principally to the surface and embryos of this parasitic nematode. Vaccination of mice with recombinant Ts -FBPA (r Ts -FBPA) resulted in a Th1/Th2 mixed humoral and cellular immune response with Th2 predominant, as well as remarkably elevated IgE levels. Moreover, mice vaccinated with r Ts -FBPA displayed a 48.7% reduction in adult worm burden and 52.5% reduction in muscle larval burden. These studies indicated that Ts -FBPA is a promising target for developing an effective vaccine to prevent and control Trichinella infection.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of Fructose-1,6-bisphosphate Aldolase From Trichinella spiralis and Its Potential in Inducing Immune Protection

Yang

Bai

et al. 2019

Front. Cell. Infect. Microbiol.

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“…The 50% inhibiting concentration (IC50) of AMP (positive control) and MB05032 against rCsFBPase or native CsFBPase in soluble protein was respectively measured at 37˚C in a reaction mixture containing different concentrations of AMP (0-50 mM) or MB05032 (0-500μM). For inhibitory specificity assay, recombinant fructose-1,6-bisphosphate aldolase-2 of C. sinensis (rCsFbA-2, GenBank accession number: DF143896.1) was prepared and used as a control [32]. Reaction was performed at 37˚C in the reaction mixture of rCsFBPase or rCsFbA-2 with or without the addition of 10 μM MB05032.…”

Section: Assay Of L-amino Acid Level In Cultured Supernatant Of Diffementioning

confidence: 99%

“…Reaction was performed at 37˚C in the reaction mixture of rCsFBPase or rCsFbA-2 with or without the addition of 10 μM MB05032. The reaction mixture of rCsFbA-2 was prepared as described [32]. Every sample was performed in triplicate and the experiment was repeated for 3 times.…”

Section: Assay Of L-amino Acid Level In Cultured Supernatant Of Diffementioning

confidence: 99%

Amino acids serve as an important energy source for adult flukes of Clonorchis sinensis

Chen

Zhou

et al. 2020

PLoS Negl Trop Dis

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Clonorchiasis, caused by chronic infection with Clonorchis sinensis (C. sinensis), is an important food-borne parasitic disease that seriously afflicts more than 35 million people globally, resulting in a socioeconomic burden in endemic regions. C. sinensis adults longterm inhabit the microaerobic and limited-glucose environment of the bile ducts. Energy metabolism plays a key role in facilitating the adaptation of adult flukes to crowded habitat and hostile environment. To understand energy source for adult flukes, we compared the component and content of free amino acids between C. sinensis-infected and uninfected bile. The results showed that the concentrations of free amino acids, including aspartic acid, serine, glycine, alanine, histidine, asparagine, threonine, lysine, hydroxylysine, and urea, were significantly higher in C. sinensis-infected bile than those in uninfected bile. Furthermore, exogenous amino acids could be utilized by adult flukes via the gluconeogenesis pathway regardless of the absence or presence of exogenous glucose, and the rate-limiting enzymes, such as C. sinensis glucose-6-phosphatase, fructose-1,6-bisphosphatase, phosphoenolpyruvate carboxykinase, and pyruvate carboxylase, exhibited high expression levels by quantitative real-time PCR analysis. Interestingly, no matter whether exogenous glucose was present, inhibition of gluconeogenesis reduced the glucose and glycogen levels as well as the viability and survival time of adult flukes. These results suggest that gluconeogenesis might play a vital role in energy metabolism of C. sinensis and exogenous amino acids probably serve as an important energy source that benefits the continued survival of adult flukes in the host. Our study will be a cornerstone for illuminating the biological characteristics of C. sinensis and the host-parasite interactions.

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“…However, fructose-bisphosphate aldolase 1 (FBA1; spot 13), fructose-bisphosphate aldolase 3 (FBAIII; spot 46), and malate dehydrogenase (MDH; spots 44, 45, and 51) were expressed with an "above high parent" patern in the "FX01 × ZNYS01" F1. In glycolysis, FBA reversibly catalyzes fructose 1,6-bisphosphate (F1,6BP) into triose phosphates dihydroxyacetone phosphate and glyceraldehyde 3-phosphate (GA3-P) [37]. The increased abundance of various isoforms of FBA indicates the enhancement of the glycolytic pathway in the hybrids.…”

Section: Carbohydrate Metabolism-related Proteinsmentioning

confidence: 99%

Comparative Proteomic Analysis of Panax ginseng C. A. Meyer × Panax quinquefolius L. Leaves and Parental Lines

Lei¹,

Wang²,

Song³

et al. 2017

Active Ingredients From Aromatic and Medicinal Plants

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Heterosis refers to the superior performance of heterozygous F1 hybrid plants with respect to those of their genetically distinct parents. Despite its wide use in crops, heterosis is seldom applied in the Panax genus, and its molecular basis remains unclear. Thus, this study is aimed to obtain hybrid F1s and identify the proteins associated with heterosis. Hybrid F1 plants and parental inbred lines were obtained using the embryo rescue technique, and the proteomes of their leaves were analyzed using two-dimensional gel electrophoresis. A total of 236 diferentially expressed proteins were found, among which 84 nonadditive proteins indicated a heterosis patern in the hybrid. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes analysis revealed that photosynthesis, carbohydrate metabolism, and protein and amino acid synthesis were the most abundant classes of nonadditive proteins. Of the proteins in these categories, 10, 6, and 4 proteins, respectively, showed above high parent expression in the hybrid leaves. These results imply that the increment in photosynthetic capacity, carbohydrate decomposition, and nitrogen ixation might be related to the heterosis of the hybrid biomass and ginsenoside production in the hybrid leaves. This study could provide a basis for hybrid breeding of the Panax genus.

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Molecular and biochemical characterizations of three fructose-1,6-bisphosphate aldolases from Clonorchis sinensis

Cited by 16 publications

References 42 publications

Molecular Characterization of Fructose-1,6-bisphosphate Aldolase From Trichinella spiralis and Its Potential in Inducing Immune Protection

Molecular Characterization of Fructose-1,6-bisphosphate Aldolase From Trichinella spiralis and Its Potential in Inducing Immune Protection

Amino acids serve as an important energy source for adult flukes of Clonorchis sinensis

Comparative Proteomic Analysis of Panax ginseng C. A. Meyer × Panax quinquefolius L. Leaves and Parental Lines

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