Molecular and biochemical evaluation of effects of malathion, phenanthrene and cadmium on Chironomus sancticaroli (Diptera: Chironomidae) larvae

Rebechi, Débora; Palacio-Cortés, Angela María; Richardi, Vinícius Sobrinho; Beltrão, Tiago; Vicentini, Maiara; Grassi, Marco Tadeu; Silva, Simone Braga da; Alessandre, Thiago; Hasenbein, Simone; Connon, Richard E.; Navarro-Silva, Mário Antônio

doi:10.1016/j.ecoenv.2021.111953

Cited by 13 publications

(3 citation statements)

References 110 publications

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“…After a 15-day exposure, zebrafish brain tissue exhibited high concentrations of malathion (500 g/L), causing significant developmental toxicity in zebrafish embryos, including bradycardia, reduced hatching rates, malformations, causing abnormal movement patterns in zebrafish larvae (Cui et al 2023 ). Malathion (0.001, 0.06, and 0.1 mg/L) also induces oxidative stress and antioxidant effects and even affects gene expression in the nerves of shaker mosquito larvae (Rebechi et al 2021 ). In addition, malathion interferes with the activities of individual organisms and affects complex metabolic processes.…”

Section: Introductionmentioning

confidence: 99%

Mechanisms of oxidative response during biodegradation of malathion by S. oneidensis MR-1

Pan,

Li,

Zhang

et al. 2024

Environ Sci Pollut Res

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Malathion, an extensively used organophosphorus pesticide, poses a high potential risk of toxicity to humans and the environment. Shewanella (S.) oneidensis MR-1 has been proposed as a strain with excellent bioremediation capabilities, capable of efficiently removing a wide range of hard-to-degrade pollutants. However, the physiological and biochemical response of S. oneidensis MR-1 to malathion is unknown. Therefore, this study aimed to examine how S. oneidensis MR-1 responds physiologically and biochemically to malathion while also investigating the biodegradation properties of the pesticide. The results showed that the 7-day degradation rates of S. oneidensis MR-1 were 84.1, 91.6, and 94.0% at malathion concentrations of 10, 20, and 30 mg/L, respectively. As the concentration of malathion increased, superoxide dismutase and catalase activities were inhibited, leading to a significant rise in malondialdehyde content. This outcome can be attributed to the excessive production of reactive oxygen species (ROS) triggered by malathion stress. In addition, ROS production stimulates the secretion of soluble polysaccharides, which alleviates oxidative stress caused by malathion. Malathion-induced oxidative damage further exacerbated the changes in the cellular properties of S. oneidensis MR-1. During the initial stages of degradation, the cell density and total intracellular protein increased significantly with increasing malathion exposure. This can be attributed to the remarkable resistance of S. oneidensis MR-1 to malathion. Based on scanning electron microscopy observations, continuous exposure to contaminants led to a reduction in biomass and protein content, resulting in reduced cell activity and ultimately leading to cell rupture. In addition, this was accompanied by a decrease in Na+/K+- ATPase and Ca2+/Mg2+-ATPase levels, suggesting that malathion-mediated oxidative stress interfered with energy metabolism in S. oneidensis MR-1. The findings of this study provide new insights into the environmental risks associated with organophosphorus pesticides, specifically malathion, and their potential for bioremediation. Graphical Abstract

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Section: Introductionmentioning

confidence: 99%

Mechanisms of oxidative response during biodegradation of malathion by S. oneidensis MR-1

Pan,

Li,

Zhang

et al. 2024

Environ Sci Pollut Res

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“…Chironomid larvae are considered models in toxicity tests due to their wide distribution in aquatic environments, ease of cultivation in the laboratory, short life cycle and their biological characteristics, which confer the ability to adapt to adverse environments (OECD/ OECD, 2010 Rosa et al 2014;Serra et al 2017). The species Chironomus sancticaroli has been widely used in the literature as a bioindicator of environmental quality with several pollutants and chemical compounds, including MP (Pinto et al 2021;Rebechi et al 2021;Palacio-Cortés et al 2022). The life cycle of C. sancticaroli is characterized by four stages: egg, larva, pupa and adult (aerial).…”

Section: Introductionmentioning

confidence: 99%

Biological responses of Chironomus sancticaroli to exposure to naturally aged PP microplastics under realistic concentrations

et al. 2023

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Microplastic (MP) is yet another form of chronic anthropogenic contribution to the environment. MP are plastic particles (< 5 mm) that have been widely found in the most diverse natural environments, but their real impacts on ecosystems are still under investigation. Here, we studied the toxicity of naturally aged secondary polypropylene (PP) MP after constant exposure to ultraviolet radiation (26 µm) to larvae of Chironomus sancticaroli, a dipteran species. The concentrations tested were 13.5; 67.5; and 135 items.g -1 of dry sediment. C. sancticaroli organisms were investigated for fragment ingestion, mortality and changes in enzymatic biomarkers after 144 hours of exposure. The organisms were able to ingest MP from the rst 48 hours, and the amount of items internalized was dose-dependent and time-dependent. Overall, the results show that mortality was low, being signi cant at the lowest and highest concentrations (13.5 and 135 items.g -1 ). Regarding changes in enzyme markers, after 144 hours MDA and CAT activities were both signi cantly altered (increased and reduced, respectively), while SOD and GST levels were unchanged. In the present study, naturally aged polypropylene MP induced biochemical toxicity in C. sancticaroli larvae, with toxicity being higher according to exposure time and particle concentration.

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“…After a 15-day exposure, zebra sh brain tissue exhibited high concentrations of malathion (500 g/L), causing signi cant developmental toxicity in zebra sh embryos, including bradycardia, reduced hatching rates, malformations, causing abnormal movement patterns in zebra sh larvae (Cui et al, 2023). Malathion (0.001, 0.0564, and 0.1006 mg/L) also induces oxidative stress and antioxidant effects and even affects gene expression in the nerves of shaker mosquito larvae (Rebechi et al, 2021). In addition, malathion interferes with the activities of individual organisms and affects complex metabolic processes.…”

Section: Introductionmentioning

confidence: 99%

Mechanisms of oxidative response during biodegradation of malathion by S. oneidensis MR-1

Pan,

Li,

Zhang

et al. 2023

Preprint

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Malathion, an extensively used organophosphorus pesticide, poses a high potential risk of toxicity to humans and the environment. Shewanella (S.) oneidensis MR-1 has been proposed as a strain with excellent bioremediation capabilities, capable of efficiently removing a wide range of hard-to-degrade pollutants. However, the physiological and biochemical response of S. oneidensis MR-1 to malathion is unknown. Therefore, this study aimed to examine how S. oneidensis MR-1 responds physiologically and biochemically to malathion while also investigating the biodegradation properties of the pesticide. The results showed that the 7-day degradation rates of S. oneidensis MR-1 were 84.085, 91.562, and 94.014% at malathion concentrations of 10, 20, and 30 mg/L, respectively. As the concentration of malathion increased, superoxide dismutase and catalase activities were inhibited, leading to a significant rise in malondialdehyde content. This outcome can be attributed to the excessive production of reactive oxygen species (ROS) triggered by malathion stress. In addition, ROS production stimulates the secretion of soluble polysaccharides, which alleviates oxidative stress caused by malathion. Malathion-induced oxidative damage further exacerbated the changes in the cellular properties of S. oneidensis MR-1. During the initial stages of degradation, the cell density and total intracellular protein increased significantly with increasing malathion exposure. This can be attributed to the remarkable resistance of S. oneidensis MR-1 to malathion. Based on scanning electron microscopy observations, continuous exposure to contaminants led to a reduction in biomass and protein content, resulting in reduced cell activity and ultimately leading to cell rupture. In addition, this was accompanied by a decrease in Na+/ K+- ATPase and Ca2+ / Mg2+- ATPase levels, suggesting that malathion-mediated oxidative stress interfered with energy metabolism in S. oneidensis MR-1. The findings of this study provide new insights into the environmental risks associated with organophosphorus pesticides, specifically malathion, and their potential for bioremediation.

show abstract

Molecular and biochemical evaluation of effects of malathion, phenanthrene and cadmium on Chironomus sancticaroli (Diptera: Chironomidae) larvae

Cited by 13 publications

References 110 publications

Mechanisms of oxidative response during biodegradation of malathion by S. oneidensis MR-1

Mechanisms of oxidative response during biodegradation of malathion by S. oneidensis MR-1

Biological responses of Chironomus sancticaroli to exposure to naturally aged PP microplastics under realistic concentrations

Mechanisms of oxidative response during biodegradation of malathion by S. oneidensis MR-1

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