Molecular and biochemical mechanisms associated with differential responses to drought tolerance in wheat (<i>Triticum aestivum</i>L.)

Islam, Monirul; Begum, Most Champa; Kabir, Ahmad Humayan; Alam, Mohammad Firoz

doi:10.1080/17429145.2015.1064174

Cited by 21 publications

(14 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Drought stress is the most serious environmental stress posing a severe threat to maize production worldwide [ 9 , 10 , 11 , 16 , 40 ]. In response to drought stress, plants evolve complex adaptive mechanisms at the physiological, biochemical, and molecular levels [ 41 , 42 , 43 , 44 , 45 ]. To gain in-depth understanding of the determinants underpinning drought tolerance in maize, herein, we have performed proteomic analysis of two contrasting maize inbred lines (drought-tolerant YE8112 and drought-sensitive MO17) after a seven-day moisture-deficit exposure period at the seedling stage.…”

Section: Discussionmentioning

confidence: 99%

Comparative Proteomic and Physiological Analyses of Two Divergent Maize Inbred Lines Provide More Insights into Drought-Stress Tolerance Mechanisms

Zenda

Liu

Wang

et al. 2018

IJMS

View full text Add to dashboard Cite

Drought stress is the major abiotic factor threatening maize (Zea mays L.) yield globally. Therefore, revealing the molecular mechanisms fundamental to drought tolerance in maize becomes imperative. Herein, we conducted a comprehensive comparative analysis of two maize inbred lines contrasting in drought stress tolerance based on their physiological and proteomic responses at the seedling stage. Our observations showed that divergent stress tolerance mechanisms exist between the two inbred-lines at physiological and proteomic levels, with YE8112 being comparatively more tolerant than MO17 owing to its maintenance of higher relative leaf water and proline contents, greater increase in peroxidase (POD) activity, along with decreased level of lipid peroxidation under stressed conditions. Using an iTRAQ (isobaric tags for relative and absolute quantification)-based method, we identified a total of 721 differentially abundant proteins (DAPs). Amongst these, we fished out five essential sets of drought responsive DAPs, including 13 DAPs specific to YE8112, 107 specific DAPs shared between drought-sensitive and drought-tolerant lines after drought treatment (SD_TD), three DAPs of YE8112 also regulated in SD_TD, 84 DAPs unique to MO17, and five overlapping DAPs between the two inbred lines. The most significantly enriched DAPs in YE8112 were associated with the photosynthesis antenna proteins pathway, whilst those in MO17 were related to C5-branched dibasic acid metabolism and RNA transport pathways. The changes in protein abundance were consistent with the observed physiological characterizations of the two inbred lines. Further, quantitative real-time polymerase chain reaction (qRT-PCR) analysis results confirmed the iTRAQ sequencing data. The higher drought tolerance of YE8112 was attributed to: activation of photosynthesis proteins involved in balancing light capture and utilization; enhanced lipid-metabolism; development of abiotic and biotic cross-tolerance mechanisms; increased cellular detoxification capacity; activation of chaperones that stabilize other proteins against drought-induced denaturation; and reduced synthesis of redundant proteins to help save energy to battle drought stress. These findings provide further insights into the molecular signatures underpinning maize drought stress tolerance.

show abstract

Section: Discussionmentioning

confidence: 99%

Comparative Proteomic and Physiological Analyses of Two Divergent Maize Inbred Lines Provide More Insights into Drought-Stress Tolerance Mechanisms

Zenda

Liu

Wang

et al. 2018

IJMS

View full text Add to dashboard Cite

show abstract

“…Among them, CRT is a unique endoplasmic reticulum (ER) luminal Ca 2+ binding chaperone, which plays a role in many cellular processes [76]. The increase of CRT abundance enhanced the survival of T. aestivum plants under drought condition [77], and the TaCRT -overexpressing tobacco ( Nicotiana benthamiana ) plants exhibited enhanced drought resistance [78]. Proteomic studies revealed a drought-induced CRT1 in Glycine max [17], but a drought-reduced CRT1 in Quercus robur under prolonged (65 days) drought stress [61].…”

Section: Drought Sensing and Signalingmentioning

confidence: 99%

Drought-Responsive Mechanisms in Plant Leaves Revealed by Proteomics

Wang

Cai

et al. 2016

IJMS

213

143

View full text Add to dashboard Cite

Plant drought tolerance is a complex trait that requires a global view to understand its underlying mechanism. The proteomic aspects of plant drought response have been extensively investigated in model plants, crops and wood plants. In this review, we summarize recent proteomic studies on drought response in leaves to reveal the common and specialized drought-responsive mechanisms in different plants. Although drought-responsive proteins exhibit various patterns depending on plant species, genotypes and stress intensity, proteomic analyses show that dominant changes occurred in sensing and signal transduction, reactive oxygen species scavenging, osmotic regulation, gene expression, protein synthesis/turnover, cell structure modulation, as well as carbohydrate and energy metabolism. In combination with physiological and molecular results, proteomic studies in leaves have helped to discover some potential proteins and/or metabolic pathways for drought tolerance. These findings provide new clues for understanding the molecular basis of plant drought tolerance.

show abstract

“…Phytochelatin (PC) and 2-DMA were tested in roots and leaves by HPLC (Binary Gradient HPLC System, Waters Corporation, Milford, Massachusetts, USA) with Empower2 ™ software as previously described with some modifications (Lindberg et al 2007;Nishiyama et al 2012;Islam et al 2015;Kabir et al 2015). Briefly, samples were ground in chilled mortar pestle in deionized water.…”

Section: Analysis Of Pc and Dma By Hplc (High-performance Liquid Chromentioning

confidence: 99%

“…Plant metabolites were analyzed in leaves by an LCMS-2020 system equipped with prominence HPLC system (Shimadzu, Kyoto, Japan) and mass spectrometry (4000 Qtrap, AB Sciex) in ESI positive ion mode as previously described with some modifications (Murakami et al 2014;Islam et al 2015). Briefly, a mobile-phase column (EZ:faast AAA-MS column 250 × 2.0 mm) was eluted (0.25 mL/min) with gradient of 68% (0-13 min), 83% (13-13.1 min) and 68% (13-17 min) acetonitrile containing 10 mM ammonium formate in water at 35°C.…”

Section: Analysis Of Metabolites By Lc-ms (Liquid Chromatography-massmentioning

confidence: 99%

Biochemical and molecular mechanisms associated with Zn deficiency tolerance and signaling in rice (Oryza sativa L.)

Kabir

Hossain

Khatun

et al. 2017

Journal of Plant Interactions

Self Cite

View full text Add to dashboard Cite

In this study, zinc (Zn) deficiency caused a significant reduction in growth parameters and tissue Zn concentrations in BRRI 33 (sensitive) but not in Pokkali (tolerant). The increase of proton extrusion in both genotypes under high pH suggests that it gets triggered as a common consequence of reducing pH and solubilization of Zn. Real-time PCR showed pronounced upregulation of OsZIP4, OsDMAS1, OsNAS2 and OsPCS1 in Zn-deficient roots of Pokkali, and to a lesser extent in BRRI 33 only for OsZIP4 and OsPCS1. This suggests that OsDMAS1, OsNAS2 and OsPCS1 functions as secondary consequences leading to higher chelation and uptake of Zn under Zn deficiency in Pokkali. Further, a major increase in CAT, POD, SOD, GR and key metabolites suggests that high antioxidant defense plays a critical role in Zn deficiency tolerance in Pokkali. Further, Pokkali selfgrafts and plants having Pokkali rootstock combined with BRRI 33 scion showed no significant decline in plant height, root dry matter and Zn concentration along with upregulation of Zn transporters (OsZIP4 and OsIRT1) under Zn deficiency, suggesting that signal driving mechanisms for Zn deficiency tolerance mechanisms are generated in the root and Zn-inefficient BRRI 33 is not capable of producing signals or sensing them. ARTICLE HISTORY

show abstract

Molecular and biochemical mechanisms associated with differential responses to drought tolerance in wheat (Triticum aestivumL.)

Cited by 21 publications

References 27 publications

Comparative Proteomic and Physiological Analyses of Two Divergent Maize Inbred Lines Provide More Insights into Drought-Stress Tolerance Mechanisms

Comparative Proteomic and Physiological Analyses of Two Divergent Maize Inbred Lines Provide More Insights into Drought-Stress Tolerance Mechanisms

Drought-Responsive Mechanisms in Plant Leaves Revealed by Proteomics

Biochemical and molecular mechanisms associated with Zn deficiency tolerance and signaling in rice (Oryza sativa L.)

Contact Info

Product

Resources

About