Molecular and biological assessment reveals sources of resistance to Plum pox virus - Turkey strain in Turkish apricot (Prunus armeniaca) germplasm

Gürcan, Kahraman; Çetinsağ, Necati; Pınar, Hasan; Macit, Tahir

doi:10.1016/j.scienta.2019.04.003

Cited by 9 publications

(2 citation statements)

References 36 publications

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“…To cope with this issue, germplasm banks are fundamental in preserving genetic diversity and avoiding gene loss. Genetically diverse germplasm can provide useful genes for enhancing pest resistance and disease tolerance and for breeding cultivars with new fruit quality traits and improved postharvest shelf-life [7][8][9][10]. Effective utilization of Prunus accessions in breeding programs requires precise and unambiguous characterization.…”

Section: Introductionmentioning

confidence: 99%

Exploring Genome-Wide Diversity in the National Peach (Prunus persica) Germplasm Collection at CITA (Zaragoza, Spain)

et al. 2021

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Peach (Prunus persica (L.) Batsch) is one of the most produced and studied stone fruits. Many genetic and genomic resources are available for this species, including a high-quality genome. More recently, a new high-density Illumina peach Single Nucleotide Polymorphism (SNP) chip (9+9K) has been developed by an international consortium as an add-on to the previous 9K array. In the current study, this new array was used to study the genetic diversity and population structure of the National Peach Germplasm Collection of the Agrifood Research and Technology Centre of Aragon (CITA), located in Zaragoza (northern Spain). To accomplish this, 90 peach accessions were genotyped using the new peach SNP chip (9+9K). A total of 9796 SNPs were finally selected for genetic analyses. Through Identity-By-Descent (IBD) estimate analysis, 15 different groups with genetically identical individuals were identified. The genetic diversity and population structure elucidated a possible exchange of germplasm material among regions, mainly in the northern regions of Spain. This study will allow for more efficient management of the National Peach Germplasm Collection by classifying valuable individuals for genetic diversity preservation and will benefit forthcoming Genome-Wide Association Studies (GWAS) of commercially important fruit traits in peach.

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Section: Introductionmentioning

confidence: 99%

Exploring Genome-Wide Diversity in the National Peach (Prunus persica) Germplasm Collection at CITA (Zaragoza, Spain)

et al. 2021

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“…The genus Prunus contains 400 species of trees and shrubs, covering some of the economically important temperate stone fruit species (Abbasi et al, 2019;Batnini et al, 2019). Prunus armeniaca L. (apricot), an economically important fruit species, is grown worldwide with a large number of varieties, mostly in temperate regions, the Mediterranean Basin, North America, and Asia (Hagen et al, 2004;Gürcan et al, 2019;Zhang et al, 2019a). Apricot is among the most commonly cultivated stone fruits in the world (Yılmaz et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Molecular Genetic Diversity of Prunus armeniaca L. (Rosaceae) Genotypes by RAPD, ISSR-PCR, and Chloroplast DNA (cpDNA) trnL-F Sequences

Sevindik

Murathan

Sevindik

2020

International Journal of Fruit Science

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In this study, phylogenetic analysis of some Turkish Prunus armeniaca L. genotypes was conducted based on RAPD-PCR, ISSR-PCR, and chloroplast DNA (trnL-F) sequence analyses. Prunus armeniaca genotypes were obtained from Malatya province, and brought to the laboratory for genomic DNA isolation. Eleven RAPD and fifteen ISSR primers were used to determine molecular characterization of Prunus armeniaca genotypes. For the amplification of the trnL-F region, trne and trnf primers were used in Polymerase Chain Reaction (PCR). A total of 46 and 95 bands were obtained with RAPD and ISSR analysis, respectively. The trnL-F sequences varied from 398 to 403 nucleotides. Average nucleotide composition of trnL-F sequences was 31.0% T, 14.4% C, 37.4% A and 17.2% G. The genetic distance between the five apricot genotypes is 0.00. A maximum likelihood tree was generated to determine genetic relationships among Prunus armeniaca genotypes. Analysis of Prunus armeniaca genotypes based on RAPD, ISSR, and trnL-F sequences revealed that the rate of polymorphism obtained using RAPD and ISSR were higher than those obtained through the trnL-F sequences.

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Genotypic diversity and population structure of the apricot landraces of the Campania region (Southern Italy) based on fluorescent SSRs

Basile

Rao

Corrado

2022

Genet Resour Crop Evol

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Apricot (Prunus armeniaca L.) is a cosmopolitan, major stone fruit species cultivated for its delicious drupes. Strong concerns about loss of diversity in apricot have been recently put forward mainly because of the enduring transition from local to globalized varieties more suitable for intensive agriculture. Italy, one of the top producing countries in the world, has arguably one of the richest apricot germplasms, with the Campania region being a traditional and leading area of cultivation since Roman times. This work aimed to study the molecular diversity of an ex-situ collection of local apricot varieties. They once flourished in conventional cropping systems, but are nowadays neglected, surviving in family farms, and sold only in local markets. We investigated the level of genetic and genotypic diversity, assessed clonality in the population, and verified the presence of genetic structure and differentiation in 73 apricot varieties by using fluorescent SSR markers resolved in capillary electrophoresis (SSR-CE). The analysis indicated a high level of genetic diversity (with little locus-specific differences) and of genotypic assortment, with few possible synonyms. Moreover, the investigation of relatedness among apricot landraces and the negligible within-landraces differentiation are consistent with a common origin of the germplasm, a likely result of an adaptive diversification that is mainly due to similar environmental and agricultural factors. This work highlights the value of the traditional germplasm where crops thrived. Moreover, it provides support for studying the possible mechanisms of evolution and adaptation and for implementing measures for the exploitation, regeneration, and on-farm promotion of the apricot landraces.

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Molecular and biological assessment reveals sources of resistance to Plum pox virus - Turkey strain in Turkish apricot (Prunus armeniaca) germplasm

Cited by 9 publications

References 36 publications

Exploring Genome-Wide Diversity in the National Peach (Prunus persica) Germplasm Collection at CITA (Zaragoza, Spain)

Exploring Genome-Wide Diversity in the National Peach (Prunus persica) Germplasm Collection at CITA (Zaragoza, Spain)

Molecular Genetic Diversity of Prunus armeniaca L. (Rosaceae) Genotypes by RAPD, ISSR-PCR, and Chloroplast DNA (cpDNA) trnL-F Sequences

Genotypic diversity and population structure of the apricot landraces of the Campania region (Southern Italy) based on fluorescent SSRs

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