Molecular and Biological Characteristics of Human Herpes Virus Type 6 in Patients With Different Variants of the Disease Course

Nikolskiy, M. A.; Vyazovaya, Anna; Vedernikov, V. E.; Narvskaya, Olga; Lioznov, Dmitry; Smirnova, N. N.; Полунина, Анна Владимировна; Бурмистрова, А Г; Zolotova, M.A.

doi:10.24110/0031-403x-2019-98-1-53-56

Cited by 8 publications

(3 citation statements)

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“…Группой исследователей из Санкт-Петербурга в 2016 г. было проведено исследование с целью определения варианта возбудителя ВГЧ-6-инфекции на территории Российской Федерации, результаты которого показали абсолютное превалирование среди вирусов ВГЧ-6B [47]. Частота инфицирования ВГЧ-6А изучена недостаточно [5].…”

Section: эпидемиология и пути передачи вгч-6a/bunclassified

Human herpes virus type 6 (Orthoherpesviridae: <i>Roseolovirus</i>): features of epidemiology and diagnosis

Saydullayeva,

Tikhomirov,

Drokov

et al. 2024

Problems of Virology

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Human herpes virus 6A and human herpes virus 6B (HHV-6A and HHV-6B) are ubiquitous viruses. The spectrum of clinical manifestations of HHV-6A/B infections is quite wide. The current understanding of the natural history and laboratory diagnosis of HHV-6A and HHV-6B, including their chromosome-integrated form, serves the basis for development of the tools for HHV-6 epidemiological monitoring. This article addresses the epidemiology and diagnosis of infections caused by these viruses, including ones in patients after transplantation of solid organs and allogeneic hematopoietic stem cells.

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Section: эпидемиология и пути передачи вгч-6a/bunclassified

Human herpes virus type 6 (Orthoherpesviridae: <i>Roseolovirus</i>): features of epidemiology and diagnosis

Saydullayeva,

Tikhomirov,

Drokov

et al. 2024

Problems of Virology

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“…The characteristic symptoms of primary HHV-6 infection are fever, enlarged occipital lymph nodes, and a typical rash that appears after the normalization of body temperature. White blood cell counts often show leukopenia or a normal count, which is an important feature for diagnostic purposes in a febrile infant [2].…”

Section: Introductionmentioning

confidence: 99%

“…In addition, up to 20-30% of febrile seizures (FS) in children are caused by a primary HHV-6 infection [6]. FS usually occurs on the first or second day of fever and affects nearly 10-15% of children with a primary HHV-6 infection [2]. A series of convulsions that occur after the normalization of body temperature at the time of the appearance of the rash is called "HHV-6 encephalopathy with cluster of convulsions in eruptive stage" (HECCES) [7].…”

Section: Introductionmentioning

confidence: 99%

HHV-6 in Cerebrospinal Fluid in Immunocompetent Children

Nikolskiy,

Lioznov,

Gorelik

et al. 2023

BioMed

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Background: Human herpes virus-6 (HHV-6) is a ubiquitous virus known as an etiological agent of exanthem subitum. HHV-6-encephalitis in immunocompetent children is a rare complication of the primary infection. There are a lack of data on the prevalence of HHV-6-encephalitis in Russia. The aim of our study was to analyze the prevalence of HHV-6 DNA in the cerebrospinal fluid (CSF) of immunocompetent children with suspected acute meningoencephalitis. Materials and Methods: A total of 712 CSF samples were tested using qualitative PCR over 12 years at N.F. Filatov Children’s City Clinical Hospital No.5, Saint-Petersburg, Russia. Results: HHV-6 DNA was detected in 20 (2.8%) of the samples. Retrospectively, we analyzed the medical records of 15 cases. There were seven boys and eight girls from 1 month to 7 years old; the mean age was 22.3 ± 5.6 months. Most HHV-6 DNA-positive cases were due to the primary form of HHV-6 infection. There were, in total, four cases of HHV-6-meningoencephalitis. All the children had a complete recovery, without neurological deficits or death. Conclusions: HHV-6 encephalitis is a very rare disease in immunocompetent children (four cases among 712 samples over 12 years). Additional studies are needed to develop accurate diagnostic criteria and therapeutic approaches.

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Development and Validation for Intra-Species Classification of Hhv6a and Hhv6b Molecular Genetic Diversity

Filatova,

Utkin,

Khrulev

et al. 2024

Russian Journal of Infection and Immunity

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Human herpesvirus 6A (HHV6A) and human herpesvirus 6B (HHV6) are ubiquitous viruses that infect more than 95% of the population. Clinical manifestations of HHV6 infection and associated diseases are diverse, which may depend on virus molecular genetic characteristics (genovariants). Estimating the significance of the molecular genetic diversity is complicated due to the lack of proper classification. The aim of the study was to develop an intraspecies HHV6A and HHV6B classification. Using 50 and 207 HHV6A and HHV6B full-genome sequences retrieved from the NCBI Nucleotide database, various fragments of virus genome were analyzed. Multiple sequence alignment was performed using MAFFT L-INS-i algorithm; F81 nucleotide substitution model and maximum likelihood method were used to construct dendrograms. Nucleotide substitutions were determined relative to reference sequences X83413 (HHV6A) and AF157706 (HHV6B). Genovariants were defined based on the nucleotide substitutions in variable positions of the genomic fragment. The results were confirmed by constructing dendrograms. An opportunity of using fragments of HHV6A and HHV6B genomes to construct an intraspecies classification was assessed. Fragments U90 (part206) and U90B(part431) were selected as optimal. Based on the nucleotide sequences of the fragments, the intraspecies classification for HHV6A and HHV6B was constructed, including seven genovariants of each virus. The genovariants were characterized by unique nucleotide composition in the signature positions. A minimum (0.001 or less for both viruses) nucleotide diversity within the isolated genovariants was established. The classification reflects the phylogenetic relationships of circulating and inherited chromosomally integrated forms of HHV6A and HHV6B: divergence of HHV6A genovariants depending on its persistence form and integration site and coevolution of two HHV6B forms within several genovariants. Further studies on virus molecular genetic diversity in different regions of Russia and abroad may supplement the classification. The method of HHV6A and HHV6B classification is characterized by simplicity, technological accessibility and can be implemented in laboratories of different levels of technical equipment. The classification can be used to analyze an effect of virus molecular genetic diversity on the clinical characteristics of associated diseases, optimize the epidemiological surveillance system and develop new approaches for diagnostics, prevention, and treatment of HHV6 infection.

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Molecular and Biological Characteristics of Human Herpes Virus Type 6 in Patients With Different Variants of the Disease Course

Cited by 8 publications

References 0 publications

Human herpes virus type 6 (Orthoherpesviridae: <i>Roseolovirus</i>): features of epidemiology and diagnosis

Human herpes virus type 6 (Orthoherpesviridae: <i>Roseolovirus</i>): features of epidemiology and diagnosis

HHV-6 in Cerebrospinal Fluid in Immunocompetent Children

Development and Validation for Intra-Species Classification of Hhv6a and Hhv6b Molecular Genetic Diversity

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