Molecular and biological characterization of a putative new sobemovirus infecting Physalis peruviana

Fariña, Arnaldo Esquivel; Gorayeb, Eduardo Silva; Camelo‐García, Viviana Marcela; Bonin, Jaíne; Nagata, Tatsuya; Silva, Joao Marcos F.; Bogo, Amauri; Rezende, Jorge Alberto Marques; Silva, Fábio Nascimento da; Kitajima, Elliot Watanabe

doi:10.1007/s00705-019-04374-y

Cited by 10 publications

(13 citation statements)

References 25 publications

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“…CMV was successfully transmitted to five P. peruviana plants using 20 aphids per plant, demonstrating acquisition and inoculation access periods of 10 min. PhyRMV was previously found naturally infecting P. peruviana plants in Brazil (Fariña et al, 2019, 2022). To our knowledge, this is the first report of CMV infecting P. peruviana plants in Brazil.…”

Section: Figurementioning

confidence: 96%

“…Isometric particles measuring approximately 30 nm in diameter were observed by transmission electron microscope in leaf extracts from all plants stained with 1% uranyl acetate. Total RNA was extracted from all four symptomatic plants using the PureLink Viral RNA/DNA Mini kit (Thermo Fisher Scientific) and separately subjected to a single‐step reverse transcription PCR (RT‐PCR) using specific primers for cucumber mosaic virus ‐ CMV (5'CP and 3'CP) (Rizos et al, 1992) and for physalis rugose mosaic virus ‐ PhyRMV (Sobemo1F/Sobemo1R; Fariña et al, 2019). Amplicons of the expected size for CMV (870 bp) and PhyRMV (528 bp) were obtained with total RNA from each of the four plants analysed.…”

Section: Figurementioning

confidence: 99%

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Natural occurrence of cucumber mosaic virus infecting Physalis peruviana plants in Brazil

Bello

Carpim

Lorenzi

et al. 2023

Journal of Phytopathology

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In 2020, virus‐like symptoms such as leaf size reduction, mosaic, and blistering were observed in Physalis peruviana plants in Brazil. Isometric particles ca. 30 nm in diameter were observed by transmission electron microscope in leaf extracts of field‐symptomatic plants. Based on molecular assays and nucleotide sequencing of amplicons, cucumber mosaic virus (CMV, genus Cucumovirus) was identified in mixed infection with physalis rugose mosaic virus (PhyRMV, genus Sobemovirus). Phylogenetic analysis indicated that the CMV belongs to the subgroup IA. Symptoms like those of field symptomatic plants were observed on mechanically inoculated plants of P. peruviana. RT‐PCR confirmed CMV and PhyRMV infection. CMV was transmitted by Aphis gossypii to P. peruviana plants. This is the first report of CMV infecting P. peruviana plants in Brazil.

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Section: Figurementioning

confidence: 96%

Section: Figurementioning

confidence: 99%

Natural occurrence of cucumber mosaic virus infecting Physalis peruviana plants in Brazil

Bello

Carpim

Lorenzi

et al. 2023

Journal of Phytopathology

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“…Total RNA was extracted from leaf tissues using the Purelink Viral RNA/DNA Mini kit (Thermo Fisher Scientific), following the manufacturer's protocol. For PhyRMV detection, total RNA was used for one-step RT-PCR, using the primer set Sobemo1F (5′-TAGCC AAG CTC AAT CCATTT-3′) and Sobemo1R (5′-GTCTT AGG CCA AGA AGTCAA-3′), generating an amplicon of 937 bp (Fariña et al, 2019).…”

Section: Virus Detectionmentioning

confidence: 99%

“…Like most solanaceous plants, Physalis is a target of several pathogens (Fariña et al, 2019; Ferreira et al, 2021). Viruses have gained attention due to their high incidence and difficulty in controlling their infections.…”

Section: Introductionmentioning

confidence: 99%

“…Viruses have gained attention due to their high incidence and difficulty in controlling their infections. In Brazil, five viruses have been found to naturally infect Physalis plants: two belonging to the genus Orthotospovirus , tomato chlorotic spot virus and groundnut ringspot virus (GRSV) (Eiras et al, 2012; Fariña et al, 2018), two belonging to the genus Potyvirus , potato virus Y (PVY) and pepper yellow mosaic virus (Esquivel‐Fariña et al, 2022), and one in the genus Sobemovirus , physalis rugose mosaic virus (PhyRMV), which has been reported in high incidences in the states of Santa Catarina, Paraná and São Paulo (Fariña et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Effects of physalis rugose mosaic and groundnut ringspot viruses, in single and double infections, on the development, yield and postharvest fruit characteristics of Physalis peruviana plants

et al. 2023

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Physalis peruviana (Solanaceae), originating from the Andean region of South America, is an exotic plant cultivated in Brazil that produces edible fruit. Five viruses have been reported to naturally infect the Physalis plants in Brazil, of which, two have been drawing attention for the severity of their infections: sobemovirus, physalis rugose mosaic virus (PhyRMV) and orthotospovirus, groundnut ringspot virus (GRSV). This study aimed to evaluate the effects of single and double infections of PhyRMV and GRSV on the development, yield and postharvest fruit characteristics of P. peruviana plants. We found that both viruses caused severe systemic symptoms in infected plants, and double infection even led to plant death. Plant height and aerial dry weight were significantly affected by PhyRMV and GRSV infections. Double‐infected plants were the most affected, exhibiting average height and aerial dry weight reductions of 70.9% and 89.7%, respectively. Moreover, PhyRMV‐ and GRSV‐infected plants showed average fruit yield reductions of 66.4% and 85.2%, respectively, compared to healthy plants. Notably, double‐infected plants did not produce any fruit. The fruits produced by infected plants were smaller than those produced by healthy plants. Viral infections also affected postharvest characteristics, such as titratable acidity, total soluble solids (°Brix), phenolic compound concentration and antioxidant activity. Both viruses affected the pulp firmness and palatability, but not the colour of the fruits. Therefore, effective disease management strategies against these viruses are necessary to prevent economic losses.

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Damage quantification in Physalis peruviana L. infected by the new putative sobemovirus physalis rugose mosaic virus

Gorayeb

Savi

Gonçalves

et al. 2020

Trop. plant pathol.

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A putative new virus, physalis rugose mosaic virus (PhyRMV), has been reported more frequently in Physalis peruviana associated with severe symptoms. In this study the damage caused by sap-inoculated PhyRMV in P. peruviana was assessed based on the comparison of host vegetative and reproductive parameters between virus-infected and healthy plants. Infected plants confirmed using molecular assays, showed a reduction in growth, leaf area, specific leaf area (SLA) and relative chlorophyll content. PhyRMV-infected plants yielded 70% less fruit of general lower quality parameters, except for pH, compared with the healty plants. Finally, seeds from PhyRMV-infected plants showed a reduced germination rate and, in accelerated aging assay, vigor of seeds were also significantly reduced.

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Molecular and biological characterization of a putative new sobemovirus infecting Physalis peruviana

Cited by 10 publications

References 25 publications

Natural occurrence of cucumber mosaic virus infecting Physalis peruviana plants in Brazil

Natural occurrence of cucumber mosaic virus infecting Physalis peruviana plants in Brazil

Effects of physalis rugose mosaic and groundnut ringspot viruses, in single and double infections, on the development, yield and postharvest fruit characteristics of Physalis peruviana plants

Damage quantification in Physalis peruviana L. infected by the new putative sobemovirus physalis rugose mosaic virus

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