Molecular and biological characterization of fusion regulatory proteins (FRPs): anti-FRP mAbs induced HIV-mediated cell fusion via an integrin system.

Ohta, Hiroya; Tsurudome, Masato; Matsumura, Haruo; Koga, Yuhki; Morikawa, Shigeru; Kawano, Mitsuo; Kusugawa, S.; Komada, Hiroshi; Nishio, Machiko; Ito, Yoshihiro

doi:10.1002/j.1460-2075.1994.tb06479.x

Cited by 71 publications

(71 citation statements)

References 41 publications

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“…Human SLC3a2/4F2hc/CD98hc regulates cell fusion in a variety of cell types (23)(24)(25)(26). We therefore speculated that abrogation of Slc3a2 in the zebrafish embryo might alter plasma membrane fusion during YSL formation.…”

Section: Slc3a2 Knockdown Enhances Plasma Membrane Fusion In the Ysl Andmentioning

confidence: 99%

“…Mammalian SLC3a2/4F2hc/CD98hc contributes to integrin outside-in signaling to regulate various integrin-mediated cell functions, including cell adhesion and cell-cell fusion (19,(21)(22)(23)(24). A downstream effector of integrin outside-in signaling is c-Src, which is phosphorylated at tyrosine 416 upon integrin-ligand binding (34).…”

Section: Slc3a2 Knockdown Enhances Plasma Membrane Fusion In the Ysl Andmentioning

confidence: 99%

“…Thus it is highly possible that Slc3a2 regulates microtubule dynamics via RhoA/ROCK pathway in cell-cell fusion and syncytium formation in these cells (23)(24)(25)(26).…”

Section: Slc3a2 Knockdown Enhances Plasma Membrane Fusion In the Ysl Andmentioning

confidence: 99%

“…Mammalian Slc3a2/4F2hc/CD98hc regulates integrin-mediated cell spreading, cell migration, and adhesion in mouse embryonic stem cells (19) and human placenta trophoblasts (21). Furthermore, anti-CD98hc/FRP-1 antibodies regulate virus-mediated cell fusion (23,24) and formation of multinucleated giant monocytes (25), and knockdown of Slc3a2/4F2hc/CD98hc inhibits placental syncytial trophoblast formation (26).…”

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confidence: 99%

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Solute carrier family 3 member 2 (Slc3a2) controls yolk syncytial layer (YSL) formation by regulating microtubule networks in the zebrafish embryo

Takesono

Moger

Farooq

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

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The yolk syncytial layer (YSL) in the zebrafish embryo is a multinucleated syncytium essential for embryo development, but the molecular mechanisms underlying YSL formation remain largely unknown. Here we show that zebrafish solute carrier family 3 member 2 (Slc3a2) is expressed specifically in the YSL and that slc3a2 knockdown causes severe YSL defects including clustering of the yolk syncytial nuclei and enhanced cell fusion, accompanied by disruption of microtubule networks. Expression of a constitutively active RhoA mimics the YSL phenotypes caused by slc3a2 knockdown, whereas attenuation of RhoA or ROCK activity rescues the slc3a2 -knockdown phenotypes. Furthermore, slc3a2 knockdown significantly reduces tyrosine phosphorylation of c-Src, and overexpression of a constitutively active Src restores the slc3a2 -knockdown phenotypes. Our data demonstrate a signaling pathway regulating YSL formation in which Slc3a2 inhibits the RhoA/ROCK pathway via phosphorylation of c-Src to modulate YSL microtubule dynamics. This work illuminates processes at a very early stage of zebrafish embryogenesis and more generally informs the mechanism of cell dynamics during syncytium formation.

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Section: Slc3a2 Knockdown Enhances Plasma Membrane Fusion In the Ysl Andmentioning

confidence: 99%

Section: Slc3a2 Knockdown Enhances Plasma Membrane Fusion In the Ysl Andmentioning

confidence: 99%

“…Thus it is highly possible that Slc3a2 regulates microtubule dynamics via RhoA/ROCK pathway in cell-cell fusion and syncytium formation in these cells (23)(24)(25)(26).…”

Section: Slc3a2 Knockdown Enhances Plasma Membrane Fusion In the Ysl Andmentioning

confidence: 99%

mentioning

confidence: 99%

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Solute carrier family 3 member 2 (Slc3a2) controls yolk syncytial layer (YSL) formation by regulating microtubule networks in the zebrafish embryo

Takesono

Moger

Farooq

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

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“…In addition, CD98hc binds to integrin ␤ 1A cytoplasmic domains, and this interaction correlates with CODS (14). Furthermore, clustering CD98hc activates multiple integrin-dependent functions (13,15,16) and mimics ␤ 1 integrin cosignaling in T-cells (17). Thus, CD98hc physically and functionally interacts with integrin adhesion receptors.…”

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confidence: 97%

Distinct Domains of CD98hc Regulate Integrins and Amino Acid Transport

Fenczik

Zent

Dellos

et al. 2001

Journal of Biological Chemistry

118

163

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CD98 is a cell surface heterodimer formed by the covalent linkage of CD98 heavy chain (CD98hc) with several different light chains to form amino acid transporters. CD98hc also binds specifically to the integrin ␤ 1A cytoplasmic domain and regulates integrin function. In this study, we examined the relationship between the ability of CD98hc to stimulate amino acid transport and to affect integrin function. By constructing chimeras with CD98hc and a type II transmembrane protein (CD69), we found that the cytoplasmic and transmembrane domains of CD98hc are required for its effects on integrin function, while the extracellular domain is required for stimulation of isoleucine transport. Consequently, the capacity to promote amino acid transport is not required for CD98hc's effect on integrin function. Furthermore, a mutant of CD98hc that lacks its integrin binding site can still promote increased isoleucine transport. Thus, these two functions of CD98hc are separable and require distinct domains of the protein.

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CD98 (4F2 Antigen)

Fox

2002

Wiley Encyclopedia of Molecular Medicine

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Molecular and biological characterization of fusion regulatory proteins (FRPs): anti-FRP mAbs induced HIV-mediated cell fusion via an integrin system.

Cited by 71 publications

References 41 publications

Solute carrier family 3 member 2 (Slc3a2) controls yolk syncytial layer (YSL) formation by regulating microtubule networks in the zebrafish embryo

Solute carrier family 3 member 2 (Slc3a2) controls yolk syncytial layer (YSL) formation by regulating microtubule networks in the zebrafish embryo

Distinct Domains of CD98hc Regulate Integrins and Amino Acid Transport

CD98 (4F2 Antigen)

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