Molecular and Biological Characterization of a New Strawberry Cytorhabdovirus

Fráňová, Jana; Přibylová, Jaroslava; Koloniuk, Igor

doi:10.3390/v11110982

Cited by 32 publications

(35 citation statements)

References 46 publications

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“…To further investigate the phylogenetic relationship of cytorhabdoviruses, we aligned 28 RdRP amino acid sequences (2374 aa in length, including gaps) from reference cytorhabdoviruses available in Genbank, including two sequences derived from transcriptomes of B. tabaci ( Table S2 ). The phylogenetic analysis shows that cytorhabdoviruses cluster in monophyletic groups according to its potential vector: aphid [ 35 , 36 , 37 , 38 , 39 ], planthopper [ 40 , 41 , 42 , 43 , 44 , 45 ], leafhopper [ 46 ], whitefly, and an undescribed vector ( Figure 6 ). As previously suggested [ 7 , 47 , 48 ], BaCV isolates and PpVE were closely related to B. tabaci TSA 2 (AKC57270.1), confirming that these viruses belong to the species Papaya cytorhabdovirus .…”

Section: Resultsmentioning

confidence: 99%

Transmission of the Bean-Associated Cytorhabdovirus by the Whitefly Bemisia tabaci MEAM1

Pinheiro-Lima

Pereira–Carvalho

Alves-Freitas

et al. 2020

Viruses

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The knowledge of genomic data of new plant viruses is increasing exponentially; however, some aspects of their biology, such as vectors and host range, remain mostly unknown. This information is crucial for the understanding of virus–plant interactions, control strategies, and mechanisms to prevent outbreaks. Typically, rhabdoviruses infect monocot and dicot plants and are vectored in nature by hemipteran sap-sucking insects, including aphids, leafhoppers, and planthoppers. However, several strains of a potentially whitefly-transmitted virus, papaya cytorhabdovirus, were recently described: (i) bean-associated cytorhabdovirus (BaCV) in Brazil, (ii) papaya virus E (PpVE) in Ecuador, and (iii) citrus-associated rhabdovirus (CiaRV) in China. Here, we examine the potential of the Bemisia tabaci Middle East-Asia Minor 1 (MEAM1) to transmit BaCV, its morphological and cytopathological characteristics, and assess the incidence of BaCV across bean producing areas in Brazil. Our results show that BaCV is efficiently transmitted, in experimental conditions, by B. tabaci MEAM1 to bean cultivars, and with lower efficiency to cowpea and soybean. Moreover, we detected BaCV RNA in viruliferous whiteflies but we were unable to visualize viral particles or viroplasm in the whitefly tissues. BaCV could not be singly isolated for pathogenicity tests, identification of the induced symptoms, and the transmission assay. BaCV was detected in five out of the seven states in Brazil included in our study, suggesting that it is widely distributed throughout bean producing areas in the country. This is the first report of a whitefly-transmitted rhabdovirus.

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Section: Resultsmentioning

confidence: 99%

Transmission of the Bean-Associated Cytorhabdovirus by the Whitefly Bemisia tabaci MEAM1

Pinheiro-Lima

Pereira–Carvalho

Alves-Freitas

et al. 2020

Viruses

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“…Rujana hosted five different viruses: strawberry crinkle virus (SCV; family Rhabdoviridae ; genus Cytorhabdovirus ; two genotypes), strawberry virus 1 (StrV-1; family Rhabdoviridae ; genus Cytorhabdovirus ; two genotypes), strawberry polerovirus 1 (SPV1; family Tombusviridae , genus Luteovirus ), strawberry mottle virus (SMoV; family Secoviridae ; genus Stramovirus ), and olive latent virus 1 (OLV-1; family Tombusviridae ; genus Alphanecrovirus ). The 1/2017 isolates of StrV-1 and SPV1 were described earlier [ 12 , 13 ]. Furthermore, several contigs that resembled umbraviruses were obtained.…”

Section: Resultsmentioning

confidence: 99%

“…Such high divergence of genomic sequences is not unique among viruses infecting strawberry plants. For example, three variants of a rhabdovirus StrV-1 coinfecting the same plant shared 76–87% of nt identity in their genomes [ 12 ]. At the same time, the strawberry plant was hosting three RNA1 and three RNA2 variants of a stramovirus, SMoV (GenBank accession numbers MH013322-7).…”

Section: Resultsmentioning

confidence: 99%

“…Then, 1 µL of cDNA was mixed with 10 µL of 2× PPP Master Mix (Top-Bio), 8 µL of PCR-grade H 2 O, and the 1750:1751 primers ( Table 3 ). The primers used in the study were those previously published for SMoV, SCV, SMYEV, SVBV [ 17 ], SPV1 [ 18 ] and StrV-1 [ 12 ]. Reaction mixtures devoid of cDNA templates served as no-template controls.…”

Section: Methodsmentioning

confidence: 99%

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Identification and Characterization of a Novel Umbra-like Virus, Strawberry Virus A, Infecting Strawberry Plants

Koloniuk

Přibylová

Čmejla

et al. 2022

Plants

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A novel RNA virus infecting strawberry plants was discovered using high-throughput sequencing. The analyzed plant was simultaneously infected with three different genetic variants of the virus, provisionally named strawberry virus A (StrVA). Although StrVA is phylogenetically clustered with several recently discovered, unclassified plant viruses, it has a smaller genome and several unique features in its genomic organization. A specific and sensitive qPCR system for the detection of identified StrVA genetic variants was designed. A survey conducted in the Czech Republic revealed that StrVA was present in 28.3% of strawberry samples (n = 651) from various origins (plantations, gardens, and propagation material). Sequencing of 48 randomly selected StrVA-positive strawberry samples showed that two or all three StrVA genetic variants were present in 62.5% of the samples in various proportions. StrVA was found in mixed infections with other viruses (strawberry mild yellow edge virus, strawberry crinkle virus, strawberry mottle virus, strawberry polerovirus 1, or strawberry virus 1) in 57.1% of the samples, which complicated the estimation of its biological relevance and impact on the health status of the plants.

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“…The severity of symptoms varies, and plants may also be symptomless. The virus was detected in 31% of strawberry field samples in the Czech Republic (Fránová et al, 2019). He et al (2021) found an infection rate of about 10% in samples from China, Shandong province, indicating that it may pose a potential threat to global strawberry production; however, information about its harmfulness is lacking.…”

Section: Introductionmentioning

confidence: 99%

Symptomless infection by strawberry virus 1 (StrV‐1) leads to losses in strawberry yields

et al. 2022

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High‐throughput sequencing has opened up possibilities of detecting new plant pathogens, especially viruses, with relative ease. However, information about biological characteristics and relevance to plant production is usually lacking. Recently, a new strawberry cytorhabdovirus named strawberry virus 1 (StrV‐1) was described and characterized at a molecular level. The virus can be transmitted by aphids and is commonly found in commercial plantations. Here, we studied the impact of a sole StrV‐1 infection on production in two strawberry cultivars—Darselect and Karmen. All plants infected with StrV‐1 remained symptomless during the experiment. In general, Darselect was more resilient to StrV‐1 infection, and the average yield per plant was nonsignificantly lowered by 11.7% over the 2‐year experiment. In contrast, StrV‐1 infection in cultivar Karmen led to a significant yield loss of 31.8%, indicating that the genetic background plays a role. While the average fruit weight and diameter were relatively unaffected, lower yields in infected plants stemmed from a significantly reduced number of inflorescences, by 23.6% and 29.6% in Darselect and Karmen, respectively, which resulted in a reduction in the average number of flowers per plant (decreased by 16.5% and 27.3%, respectively), and hence the number of fruits per plant (by 14.5% and 27.6%, respectively). The StrV‐1 load was higher in spring than in autumn, and generally higher in the second year after infection, which corresponded to higher losses in the second harvest, especially in cultivar Karmen.

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Molecular and Biological Characterization of a New Strawberry Cytorhabdovirus

Cited by 32 publications

References 46 publications

Transmission of the Bean-Associated Cytorhabdovirus by the Whitefly Bemisia tabaci MEAM1

Transmission of the Bean-Associated Cytorhabdovirus by the Whitefly Bemisia tabaci MEAM1

Identification and Characterization of a Novel Umbra-like Virus, Strawberry Virus A, Infecting Strawberry Plants

Symptomless infection by strawberry virus 1 (StrV‐1) leads to losses in strawberry yields

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