Molecular and biological characterization of a new Brazilian begomovirus, euphorbia yellow mosaic virus (EuYMV), infecting Euphorbia heterophylla plants

Abstract

To date, no begomovirus has been fully characterized from Euphorbia heterophylla, a widely distributed weed, in Brazil. Here, we show the occurrence of a new begomovirus on E. heterophylla plants showing bright yellow mosaic. The bipartite viral genome was cloned from 10 samples, and all clones are almost identical to each other (95.6-98.8% nucleotide sequence identity). The DNA-A sequences shared a maximum nucleotide sequence identity of 87.3% with euphorbia mosaic Peru virus (EuMPV) and thus were classified as belonging to a novel begomovirus species, tentatively named Euphorbia yellow mosaic virus (EuYMV). The EuYMV DNA-B sequences share a maximum nucleotide sequence identity of 56.2% with a euphorbia mosaic virus (EuMV) isolate from Mexico. Phylogenetic analysis demonstrated that this new virus belongs to a different lineage than EuMV isolates from Central America.

Melon chlorotic mosaic virus and associated alphasatellite from Venezuela: genetic variation and sap transmission of a begomovirus–satellite complex

“…In addition, disease outbreaks in melon crops from Colombia have been associated with a begomovirus that has been partially sequenced and seems to be a member of the SLCV clade (Morales & Anderson, 2001). Excluding MeCMV, the South American members of the SLCV clade have been isolated from Euphorbia spp., mainly Euphorbia heterophylla (Paprotka et al, 2010;Fernandes et al, 2011;Zambrano et al, 2012) which are common weeds that grow around crops (Zambrano et al, 2012). After introduction of the B biotype of B. tabaci in Latin America, local begomoviruses that were poorly transmitted by indigenous B. tabaci biotypes emerged, developing severe outbreaks due to the high transmission efficiency of the B biotype (Morales & Anderson, 2001).…”

Rad54 is not essential for any geminiviral replication mode in planta

“…After 2-4 days of acclimatization (10-12 leaf stage), viral DNA was inoculated biolistically, using rolling circle amplification (RCA) products of Euphorbia yellow mosaic virus (EuYMV; DNA A: FN435995 and DNA B: FN435996; formerly Euphorbia mosaic virus) and Cleome leaf crumple virus (ClLCrV; DNA A: FN435999 and DNA B: FN436000) (Paprotka et al 2010). Renaming of EuMV to EuYMV was implemented according to Fernandes et al (2011).…”

Cnidoscolus mosaic leaf deformation virus: a novel begomovirus infecting euphorbiaceous plants in Brazil

“…In Brazil, EuYMV has been reported to cause mosaic disease in Euphorbia heterophylla since the 1950s [11] in addition to being found naturally infecting Macroptilium atropurpureum, Sida santaremensis, Crotalaria juncea and Solanum lycopersicum [4,12,13]. To date, EuYMV is the only begomovirus infecting euphorbiaceous hosts in Brazil that has been completely characterized [8]. In 2006, begomovirus infection in Cnidoscolus urens (family Euphorbiaceae) was reported [14], with plants displaying symptoms of yellow mosaic and leaf deformation.…”

“…Previous studies have focused on the diversity of begomoviruses infecting weed plants from the family Euphorbiaceae, and several begomoviruses have been reported, including euphorbia mosaic virus [6], croton yellow vein mosaic virus [7], euphorbia yellow mosaic virus (EuYMV) [8], dalechampia chlorotic mosaic virus [9], and jatropha mosaic virus [10]. In Brazil, EuYMV has been reported to cause mosaic disease in Euphorbia heterophylla since the 1950s [11] in addition to being found naturally infecting Macroptilium atropurpureum, Sida santaremensis, Crotalaria juncea and Solanum lycopersicum [4,12,13].…”