Molecular and biological studies on male sterile cytoplasm in Cruciferae. II. The origin of Ogura male sterile cytoplasm inferred from the segregation pattern of male sterility in the F1 progeny of wild and cultivated radishes (Raphanus sativus L.)

Yamagishi, Hiroshi; Terachi, Toru

doi:10.1007/bf00039651

Cited by 20 publications

(16 citation statements)

References 16 publications

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“…The Ogura-type mtDNA is widely distributed among Japanese wild radishes, whereas most of the Asian cultivars have normal mtDNA (Yamagishi and Terachi 1996). The present study also identified Ogura-type mtDNA in R. raphanistrum.…”

Section: Origin Of Japanese Wild Radishesmentioning

confidence: 61%

“…Therefore, primer A, from which the normal atp6 locus was amplified (Yamagishi and Terachi 1994a), was used as a PCR primer. The novel primer F (5'-ATCCCTGCAGACAG-CTTCATTAC-3'), being homologous to the 3'-flanking region of orfl38, was synthesized.…”

Section: Methodsmentioning

confidence: 99%

“…The first two cultivars and 'Comet', a restorer of Ogura male sterihty, were used for the crossing experiment. Seeds were sown in pots and grown in a greenhouse as described by Yamagishi and Terachi (1994a).…”

Section: Methodsmentioning

confidence: 99%

“…Our previous studies (Yamagishi andTerachi 1994a,b, 1996) show that Ogura-type cytoplasm, which confers male sterility upon cultivated radishes, is widely distributed among Japanese wild radish plants. We also found that most Asian radish cultivars, including Japanese ones, have normal cytoplasm and that the Ogura-type is restricted to a few varieties in Tibet and Taiwan (Yamagishi and Terachi 1996). We therefore concluded that the original Ogura male-sterile radishes originated in progeny of natural hybrids between a female wild radish with Oguratype cytoplasm and a male cultivar lacking a restorer gene(s).…”

Section: Malementioning

confidence: 99%

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Molecular and biological studies on male‐sterile cytoplasm in the Cruciferae. IV. Ogura‐type cytoplasm found in the wild radish, Raphanus raphanistrum

Yamagishi

Terachi

1997

Plant Breeding

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The orfl38 gene, which is specific to Ogura male-sterile cytoplasm, was analysed in mitochondrial DNA (mtDNA) ofthe wild radish, Raphanus raphanistrum, by polymerase chain reaction (PCR), Southern hybridization and sequencing. The effect of R. raphanistrum cytoplasm on the expression of male sterihty was also examined in progeny with R. sativus. A PCR-aided assay and Southern hybridization revealed that three out of six strains analysed included plants with orfl38. The sequence of wild type orfl38 was same as that of Ogura, except for one or two nucleotide substitutions. Southern hybridization showed a novel mtDNA configuration in R. raphanistrum, in addition to the normal and Ogura types identical to those in R. sativus.Among interspecific hybrids, all the F, had normal pollen fertihty. In the F2 progeny between female wild plants having orfl38 and the maintainer of Ogura male sterihty, male-sterile plants were segregated, fitting the ratio of 3 fertile: 1 sterile plant. R. raphanistrum has cytoplasm that induces male sterihty in radishes, and contains a dominant fertiUty restorer gene.

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Section: Origin Of Japanese Wild Radishesmentioning

confidence: 61%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Malementioning

confidence: 99%

See 2 more Smart Citations

Molecular and biological studies on male‐sterile cytoplasm in the Cruciferae. IV. Ogura‐type cytoplasm found in the wild radish, Raphanus raphanistrum

Yamagishi

Terachi

1997

Plant Breeding

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show abstract

“…Yamagishi andTerachi (1996, 1997) studied the cytoplasm of radishes with the aid of PCR of mitochondrial orf138, and found that the Ogura cytoplasm is distributed widely in the wild species R. raphanistrum and Japanese wild radishes, while most cultivated radishes have a normal-type cytoplasm. They inferred from the results that the origin of the Ogura male sterility lies in the population of wild radish or the hybrids between wild and cultivated radishes (Yamagishi and Terachi, 1994b).…”

Section: Introductionmentioning

confidence: 99%

Distribution and allelism of restorer genes for Ogura cytoplasmic male sterility in wild and cultivated radishes.

Yamagishi

1998

Genes Genet. Syst.

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Brassica Ogu-INRA Cytoplasmic Male Sterility: An Example of Successful Plant Somatic Fusion for Hybrid Seed Production

Pelletier

Budar

2015

Somatic Genome Manipulation

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Molecular and biological studies on male sterile cytoplasm in Cruciferae. II. The origin of Ogura male sterile cytoplasm inferred from the segregation pattern of male sterility in the F1 progeny of wild and cultivated radishes (Raphanus sativus L.)

Cited by 20 publications

References 16 publications

Molecular and biological studies on male‐sterile cytoplasm in the Cruciferae. IV. Ogura‐type cytoplasm found in the wild radish, Raphanus raphanistrum

Molecular and biological studies on male‐sterile cytoplasm in the Cruciferae. IV. Ogura‐type cytoplasm found in the wild radish, Raphanus raphanistrum

Distribution and allelism of restorer genes for Ogura cytoplasmic male sterility in wild and cultivated radishes.

Brassica Ogu-INRA Cytoplasmic Male Sterility: An Example of Successful Plant Somatic Fusion for Hybrid Seed Production

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