BACKGROUND: In recent years, autologous serum has been increasingly used in the treatment of macular hole. The study carried out a biological analysis of autologous serum obtained by various methods.
AIM: The aim of the investigation is to compare the cellular and biochemical composition of autologous serum obtained using different methods for use in the macular hole treatment.
MATERIALS AND METHODS: The study of the number of platelets, leukocytes and fibrinogen level in the blood serum of 24 patients obtained by centrifugation in original systems for harvesting autologous serum and laboratory test tubes was performed.
RESULTS: The indices of P-PRP obtained in the Arthrex ACP system and in a laboratory test tube do not statistically differ in quantitative indices of fibrinogen and platelets (p 0.05), and differ in the content of leukocytes (p 0.05) in the direction of increasing the number of leukocytes in the substrate obtained in a laboratory test tube. The indicators of L-PRP obtained in the Ycellbio-Kit system and in a laboratory test tube do not statistically differ in the amount of fibrinogen (p 0.05) and differ in the content of platelets and leukocytes (p 0.05) in the direction of decreasing the concentration of leukocytes and platelets in the autologous serum obtained in a laboratory test tube.
CONCLUSIONS: Currently, autologous serum obtained in the Arthrex ACP system is of great interest for macular hole surgery due to the minimal content of leukocytes, the closed character of the system, and the best coagulation properties of the substrate obtained. Laboratory test tubes may be considered a more affordable alternative for the production of autologous serum (P-PRP) for the treatment of macular hole. The autologous serum (L-PRP) obtained in the Ycellbio-Kit system and in a laboratory test tube is less suitable for macular hole surgery according to its composition.
