Molecular and carbon isotopic characterization of an anaerobic stable enrichment culture containing Dehalobacterium sp. during dichloromethane fermentation

Trueba-Santiso, Alba; Parladé, Eloi; Rosell, Mònica; Llirós, Marc; Mortan, Siti Hatijah; Martínez-Alonso, Maira; Gaju, Núria; Martín-González, Lucía; Vicent, Teresa; Marco‐Urrea, Ernest

doi:10.1016/j.scitotenv.2016.12.174

Cited by 30 publications

(30 citation statements)

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“…Samples of the time-course degradation of DCM in a culture containing Dehalobacterium (Dhb) were obtained from a previous study reported elsewhere (Trueba-Santiso et al, 2017).…”

Section: Methodsmentioning

confidence: 99%

“…Samples of a Dhb-containing culture that were previously killed to analyse the decrease of concentration and the carbon isotope fractionation of DCM (Trueba-Santiso et al, 2017) were used in this study. The δ 37 Cl during DCM degradation increased from -5.7 ± 0.2 to +7.2 ± 0.2‰ after 97% transformation, but neither changes in concentration nor in δ 37 Cl (average -5.7 ± 0.2‰) were observed in the abiotic controls.…”

Section: Chlorine Isotope Fractionation and λ C/cl During Anaerobic Dmentioning

confidence: 99%

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Use of dual element isotope analysis and microcosm studies to determine the origin and potential anaerobic biodegradation of dichloromethane in two multi-contaminated aquifers

Blázquez-Pallí

Shouakar-Stash

Palau

et al. 2019

Science of The Total Environment

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Many aquifers around the world are impacted by toxic chlorinated methanes derived from industrial processes due to accidental spills. Frequently, these contaminants co-occur with chlorinated ethenes and/or chlorinated benzenes in groundwater, forming complex mixtures that become very difficult to remediate. In this study, a multi-method approach was used to provide lines of evidence of natural attenuation processes and potential setbacks in the implementation of bioremediation strategies in multi-contaminated aquifers. First, this study determined i) the carbon and chlorine isotopic compositions (δ 13 C, δ 37 Cl) of several commercial pure phase chlorinated compounds, and ii) the chlorine isotopic fractionation (εCl =-5.2 ± 0.6‰) and the dual C-Cl isotope correlation (Λ C/Cl = 5.9 ± 0.3) during dichloromethane (DCM) degradation by a Dehalobacteriumcontaining culture. Such data provide valuable information for practitioners to support the interpretation of stable isotope analyses derived from polluted sites. Second, the bioremediation potential of two industrial sites contaminated with a mixture of organic pollutants (mainly DCM, chloroform (CF), trichloroethene (TCE), and monochlorobenzene (MCB)) was evaluated. Hydrochemistry, dual (C-Cl) isotope analyses, laboratory microcosms, and microbiological data were used to investigate the origin, fate and biodegradation potential of chlorinated methanes. At Site 1, δ 13 C and δ 37 Cl compositions from field samples were consistent with laboratory microcosms, which showed complete degradation of CF, DCM and TCE, while MCB remained.

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“…Samples of the time-course degradation of DCM in a culture containing Dehalobacterium (Dhb) were obtained from a previous study reported elsewhere (Trueba-Santiso et al, 2017).…”

Section: Methodsmentioning

confidence: 99%

Section: Chlorine Isotope Fractionation and λ C/cl During Anaerobic Dmentioning

confidence: 99%

Use of dual element isotope analysis and microcosm studies to determine the origin and potential anaerobic biodegradation of dichloromethane in two multi-contaminated aquifers

Blázquez-Pallí

Shouakar-Stash

Palau

et al. 2019

Science of The Total Environment

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“…Yet study of anaerobic DCM degradation has been hindered by the highly limited number of organisms capable of this metabolism. Dehalobacterium formicoaceticum strain DMC is the only isolate (15) and from the handful of enrichment cultures (16)(17)(18), only 'Candidatus Dichloromethanomonas elyunquensis' strain RM has been characterised (19,20). Both D.…”

Section: Introductionmentioning

confidence: 99%

A novel, dichloromethane-fermenting bacterium in thePeptococcaceaefamily, ‘CandidatusFormamonas warabiya’, gen. nov. sp. nov.

Holland

Ertan

Manefield

et al. 2020

Preprint

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Dichloromethane (DCM; CH2Cl2) is a toxic groundwater pollutant that also has a detrimental effect on atmospheric ozone levels. As a dense non-aqueous phase liquid, DCM migrates vertically through groundwater to low redox zones, yet information on anaerobic microbial DCM transformation remains scarce due to a lack of cultured organisms. We report here the characterisation of strain DCMF, the dominant organism in an enrichment culture (DFE) that is capable of fermenting DCM to the environmentally benign product acetate. Stable carbon isotope experiments demonstrated that the organism assimilated carbon from DCM and bicarbonate via the Wood-Ljungdahl pathway. Strain DCMF is the first non-obligate anaerobic DCM-degrading bacterium. An anaerobic methylotroph, it appears to utilise the Wood-Ljungdahl pathway for metabolism of methyl groups from methanol, choline, and glycine betaine, which has implications for the flux of climate-active compounds from subsurface environments. Community profiling and enrichment of the cohabiting taxa in culture DFE to the exclusion of strain DCMF suggest that it is the sole organism in this culture responsible for substrate metabolism, while the cohabitants persist via necromass recycling. Genomic and physiological evidence support placement of strain DCMF in a novel genus, 'Candidatus Formamonas warabiya'.

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“…and/or Dehalobacterium sp. [4][5][6], and a new bacterium identified as Candidatus Dichloromethanomonas elyunquensis [7,8].…”

Section: Introductionmentioning

confidence: 99%

“…Previously, we reported carbon isotopic fractionation of DCM (εC = −27 ± 2‰) of an anaerobic enrichment culture containing Dehalobacterium sp. during DCM fermentation and differing from Dehalobacter and methylotrophic organisms [4]. Recently, several laboratory studies have applied C-Cl dual isotope analysis to characterize DCM biodegradation by different bacteria [13,14].…”

Section: Introductionmentioning

confidence: 99%

Use of C–Cl CSIA to elucidate origin and fate of DCM in complex contaminated field sites

et al. 2019

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We used C-Cl dual isotope analysis and microcosm studies for elucidating the origin and fate of the common groundwater pollutant dichloromethane (DCM) in two different multi-contaminant field sites in Catalonia, Spain; where DCM contamination could be the result of direct solvent releases and/or chloroform (CF) transformation. Known commercial solvents isotopic compositions as well as characteristic C-Cl dual isotope slopes from our anaerobic enrichment culture containing Dehalobacterium sp., capable of fermenting DCM, and other bacteria from the literature were used for field data interpretation.

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Molecular and carbon isotopic characterization of an anaerobic stable enrichment culture containing Dehalobacterium sp. during dichloromethane fermentation

Cited by 30 publications

References 45 publications

Use of dual element isotope analysis and microcosm studies to determine the origin and potential anaerobic biodegradation of dichloromethane in two multi-contaminated aquifers

Use of dual element isotope analysis and microcosm studies to determine the origin and potential anaerobic biodegradation of dichloromethane in two multi-contaminated aquifers

A novel, dichloromethane-fermenting bacterium in thePeptococcaceaefamily, ‘CandidatusFormamonas warabiya’, gen. nov. sp. nov.

Use of C–Cl CSIA to elucidate origin and fate of DCM in complex contaminated field sites

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