Molecular and cellular features of esophageal cancer cells

Abstract

More than 70 cell lines were established from esophageal cancer, including 15 TE-series cell lines established by the authors. This article reviews molecular and cellular features of esophageal cancer cells from studies using these cell lines as well as primary tumors. The subjects reviewed include primary cultures of normal epithelium of the esophagus and of esophageal tumors, their growth and differentiation properties, chromosomal aberrations, protein kinase C, growth factors and their receptors, oncogenes, and tumor-suppressor genes. Lesions of genetic loci in esophageal cancer include the absence of mutations in ras genes in primary tumors, amplification and overexpression of the c-erbB gene, co-amplification of hst-1 and int-2 genes, mutations, and allelic loss of tumor suppressor genes, p53, Rb, APC, and MCC. Future clinical improvement will be achieved on the basis of the understanding of molecular and cellular features of esophageal cancer cells.

Altered LKB1/CREB-regulated transcription co-activator (CRTC) signaling axis promotes esophageal cancer cell migration and invasion

“…Het1A cells were grown in BEGM medium (Biowhittaker Inc, Chesterbrook, PA, USA). The TE series (Nishihira et al, 1993) and KYSE series (Shimada et al, 1992) of esophageal cancer cells were grown in RPMI-1640 containing 10% fetal bovine serum. Retroviral and lentiviral production and transduction were previously described .…”

FAK overexpression is correlated with tumour invasiveness and lymph node metastasis in oesophageal squamous cell carcinoma

“…The TTn cell line was established from a transplanted tumour in a nude mouse, the primary lesion being the same as that from which the TT cell line was established. It has been reported that all these cell lines were transplantable in nude mice (Takahashi et al, 1990;Galiana et al, 1993;Nishihira et al, 1993). TE-2, TE-8, and TE-13 have the wild type of p53 (Barnas et al, 1997;Itoshima et al, 2000;Akimoto et al, 2001).…”

Differential gene expression profiles of radioresistant oesophageal cancer cell lines established by continuous fractionated irradiation

“…The human oesophageal squamous cancer cell lines TE-2, TE-5, TE-9, and TE-13 were obtained from the Cell Resource Center for Biomedical Research Institute of Development, Aging and Cancer (Tohoku University, Sendai, Japan) (Nishihira et al, 1993). Cell lines KYSE170 and KYSE180 were kindly provided by Dr Shimada (Kyoto University, Kyoto, Japan) (Shimada et al, 1992).…”