Molecular and Chemical Engineering of Bacteriophages for Potential Medical Applications

Hodyra, Katarzyna; Dąbrowska, Krystyna

doi:10.1007/s00005-014-0305-y

Cited by 10 publications

(11 citation statements)

References 86 publications

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“…The mononuclear phagocyte system contributes to phage clearance. The uptake of bacteriophages by mammalian cells has been reported previously [14,[25][26][27][28]. Here, we investigated the interaction of RFP-labelled phages with human macrophages.…”

Section: Discussionmentioning

confidence: 97%

Circulation of Fluorescently Labelled Phage in a Murine Model

Kaźmierczak

Majewska

Milczarek

et al. 2021

Viruses

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Interactions between bacteriophages and mammals strongly affect possible applications of bacteriophages. This has created a need for tools that facilitate studies of phage circulation and deposition in tissues. Here, we propose red fluorescent protein (RFP)-labelled E. coli lytic phages as a new tool for the investigation of phage interactions with cells and tissues. The interaction of RFP-labelled phages with living eukaryotic cells (macrophages) was visualized after 20 min of co-incubation. RFP-labeled phages were applied in a murine model of phage circulation in vivo. Phages administered by three different routes (intravenously, orally, rectally) were detected through the course of time. The intravenous route of administration was the most efficient for phage delivery to multiple body compartments: 20 min after administration, virions were detected in lymph nodes, lungs, and liver; 30 min after administration, they were detectable in muscles; and 1 h after administration, phages were detected in spleen and lymph nodes. Oral and rectal administration of RFP-labelled phages allowed for their detection in the gastrointestinal (GI) tract only.

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Section: Discussionmentioning

confidence: 97%

Circulation of Fluorescently Labelled Phage in a Murine Model

Kaźmierczak

Majewska

Milczarek

et al. 2021

Viruses

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“…Moreover, transferrin is a large and complex glycoprotein and displaying of it in high copy numbers on the phage surface with the common phage display approach seems to be impossible. Assembling of phage particles in the case of using coat proteins fused with bigger and complex proteins would be insufficient (Ivanenkov et al, 1999b;Gupta et al, 2003;Hodyra and Dąbrowska, 2015).…”

Section: Discussionmentioning

confidence: 99%

Preparation of a transferrin-targeted M13-based gene nanocarrier and evaluation of its efficacy for gene delivery and expression in eukaryote cells

Khalaj‐Kondori¹,

Kavoosi²,

Rahmati-Yamchi³

et al. 2016

Turk J Biol

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Bacteriophages are appropriate gene carriers that might be targeted toward target cells using different strategies. Here we prepared a transferrin-targeted M13-based gene nanocarrier (Tf-targeted M13-GFP) and examined its gene delivery and expression efficacy in the AGS cell line. M13 phagemid particles bearing a GFP expression cassette (M13-GFP) were obtained from a recombinant lambda phage through an in vivo excision procedure. Chemical coupling of human holotransferrin molecules (Tf) to the surface of these phagemid particles resulted in Tf-targeted M13-GFP formation, which was then characterized by Phage-ELISA and Cell-ELISA experiments. Immunocytochemistry (ICC) and fluorescence-activated cell sorting (FACS) analysis were used for internalization assay and examination of gene delivery/expression efficacies in the human AGS cell line. The ELISA experiments revealed high-density attachment of Tf molecules to the surface of M13-GFP particles and ICC confirmed highly efficient internalization of the Tf-targeted M13-GFP particles into the AGS cells. Moreover, FACS analysis showed significant increase of GFP-positive cell counts in the samples treated with Tf-targeted M13-GFP (8.09%) in comparison with the samples treated with wild M13-GFP (1.2%). We conclude that this strategy might improve phage-mediated gene delivery and expression in eukaryote cells.

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“…Two types of agents to be considered in this class are bacteriophages [184][185][186][187] and enzybiotics [188][189][190][191], which have attracted substantial attention in recent times, and can be used in prophylactic and therapeutic applications in antimicrobial and anticancer protocols. Bacteriophages have been engineered for medical applications [192] in ways allowing them to retain their antibacterial activity [185] and have been used as anticancer drug delivery systems [186,192], their possible effects on anti-tumor immunity and the response to anticancer therapy must be further evaluated. The same is even more needed with regard to enzybiotics, poorly immunogenic enzymes from bacteriophages or other natural origin able to highly specifically act as antimicrobial agents [187], as our current knowledge about their potential use in anticancer strategies is extremely limited.…”

Section: Alternative Approachesmentioning

confidence: 99%

A Significant Question in Cancer Risk and Therapy: Are Antibiotics Positive or Negative Effectors? Current Answers and Possible Alternatives

Amadei

Notario

2020

Antibiotics

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Cancer is predominantly considered as an environmental disease caused by genetic or epigenetic alterations induced by exposure to extrinsic (e.g., carcinogens, pollutants, radiation) or intrinsic (e.g., metabolic, immune or genetic deficiencies). Over-exposure to antibiotics, which is favored by unregulated access as well as inappropriate prescriptions by physicians, is known to have led to serious health problems such as the rise of antibiotic resistance, in particular in poorly developed countries. In this review, the attention is focused on evaluating the effects of antibiotic exposure on cancer risk and on the outcome of cancer therapeutic protocols, either directly acting as extrinsic promoters, or indirectly, through interactions with the human gut microbiota. The preponderant evidence derived from information reported over the last 10 years confirms that antibiotic exposure tends to increase cancer risk and, unfortunately, that it reduces the efficacy of various forms of cancer therapy (e.g., chemo-, radio-, and immunotherapy alone or in combination). Alternatives to the current patterns of antibiotic use, such as introducing new antibiotics, bacteriophages or enzybiotics, and implementing dysbiosis-reducing microbiota modulatory strategies in oncology, are discussed. The information is in the end considered from the perspective of the most recent findings on the tumor-specific and intracellular location of the tumor microbiota, and of the most recent theories proposed to explain cancer etiology on the notion of regression of the eukaryotic cells and systems to stages characterized for a lack of coordination among their components of prokaryotic origin, which is promoted by injuries caused by environmental insults.

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Molecular and Chemical Engineering of Bacteriophages for Potential Medical Applications

Cited by 10 publications

References 86 publications

Circulation of Fluorescently Labelled Phage in a Murine Model

Circulation of Fluorescently Labelled Phage in a Murine Model

Preparation of a transferrin-targeted M13-based gene nanocarrier and evaluation of its efficacy for gene delivery and expression in eukaryote cells

A Significant Question in Cancer Risk and Therapy: Are Antibiotics Positive or Negative Effectors? Current Answers and Possible Alternatives

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