Molecular and Cytogenetic Criteria for Identification of Serum-Free Cell Cultures

Abstract

The information about the authenticity of newly or already established animal cell cultures is essential. By using DNA analysis six cell lines has been investigated: ЗТЗ, HeLa, McCoy, the last four was made cytogenetic analysis confirmed that serum-free cell strains McCoy-Plovdiv and HЕp-2-Plovdiv E are originally derived from McCoy and HЕp-2 cell lines. PCR -analysis demonstrated that 3T3 cells are mouse originally, HeLa, HЕp-2 and HЕp-2-Plovdiv E are with the human origin and McCoy and McCoy-Plovdiv are hybrid cells carrying mouse and human genes in their genome.