Molecular and Field Analysis of Some Barley Genotypes for Water Stress Tolerance

El-Denary, M. E.; El-Shawy, E. E.

doi:10.21608/ejgc.2014.9940

Cited by 6 publications

(4 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Based on our results, maximum fresh and dry weight was obtained at control and minimum at 20% PEG treatment (Table 3). Results of this experiment were in line with earlier findings where the fresh and dry weight of seedlings were reduced due to the declined water potential, however, the extent of reduction was greater in shoot than the roots [52,53]. Similar results were reported for other crops under water stress conditions and seedlings had reduced root and shoot length as well as fresh and dry biomass [17,41].…”

Section: Discussionsupporting

confidence: 90%

Changes in Germination and Seedling Traits of Sesame under Simulated Drought

Ahmed¹,

Kheir²,

Mehmood³

et al. 2022

Phyton

View full text Add to dashboard Cite

Drought is considered one of the leading abiotic constraints to agricultural crop production globally. Present study was conducted to assess the effects of different drought treatments (viz. Control, 10% PEG, and 20% PEG) on seed germination, germination indices, seedling traits, and drought tolerance indices of sesame. Our results showed that maximum reduction in the studied parameters was observed at higher PEG concentration (i.e., 20% PEG). As compared to control, the drought treatments viz. 10% and 20% PEG decreased the values for germination indices, such as germination percentage, coefficient of variation of germination time, germination index, and seedling vigor index. Similarly, for seedling traits, the values were decreased for root length, shoot length, root shoot ratio, root fresh weight, shoot fresh weight, root dry weight and shoot dry weight under 10% and 20% PEG treatments significantly in comparison with control. Furthermore, relative to control, the values for drought tolerance indices, such as germination drought tolerance index, root length drought tolerance index, shoot length drought tolerance index, total seedling length drought tolerance index, root fresh weight drought tolerance index, shoot fresh weight drought tolerance index, total fresh weight drought tolerance index, root dry weight drought tolerance index, shoot dry weight drought tolerance index and total dry weight drought tolerance index were also reduced under 10% and 20% PEG treatments, respectively. Our results confirms that drought impact on seed germination and seedling traits could be quantified by using different indices which can further help to design drought adaptation and mitigation strategies. Based on these results it can be concluded that germination indices, seedling traits, and drought tolerance indices have great potential to simulate drought stress impacts on different crop traits thus they should be used in all kinds of stress related studies.

show abstract

Section: Discussionsupporting

confidence: 90%

Changes in Germination and Seedling Traits of Sesame under Simulated Drought

Ahmed¹,

Kheir²,

Mehmood³

et al. 2022

Phyton

View full text Add to dashboard Cite

show abstract

“…This negative effect was observed in descending order, the following barley cultivars Giza 124 > Giza 129 > Giza 135 > Giza 125 relative to their values at PEG at 10% and 20%. Total dry mater of root and shoot, germination percentage, shoot length, root length were the most effective traits between sensitive and tolerant barley cultivars [17].…”

Section: Resultsmentioning

confidence: 94%

“…El-Denary and El-Shawy [17] studied the water stress induced by PEG application on three barley ( Hordeum vulgare L.) genotypes. Results showed that germination percentage, shoot length, root length and total dry mater were the most effective traits between sensitive and tolerant genotypes.…”

Section: Introductionmentioning

confidence: 99%

Influence of PEG induced drought stress on molecular and biochemical constituents and seedling growth of Egyptian barley cultivars

Hellal

El-Shabrawi

El-Hady

et al. 2018

Journal of Genetic Engineering and Biotechnology

125

View full text Add to dashboard Cite

In order to investigate the effects of drought stress on germination components of barley cultivars, a laboratory experiment was conducted in a factorial randomized complete design with four replications. The controlled experiment included ten of Egyptian barley cultivars namely; (Giza 123, 124, 125, 126, 127, 129, 130, 134, 135 and 2000) as first factor. The second factor included 4 levels of drought stress inducer by applying 0, 5, 10 and 20% of polyethylene glycol-6000 (PEG) which is equivalent to four osmotic potential levels including −0.001, −0.27, −0.54 and −1.09 MPa, respectively. The results showed that, the highest reduction was related to the drought level of 20% PEG among the barley cultivars. The best cultivars in terms of germination traits were Giza 134, Giza 127, and Giza 126 this indicate their tolerance to drought stress and Giza 130, 135, 2000 cultivars was moderately tolerance and remaining is less tolerance. The protein band 27 kDa and 78 kDa showed high intensity after stress in almost all cultivars. Those two protein bands their exciting was very clear in treated barley leaf tissue. It could be related to dehydrine and oxygen evolving enhancer protein 2 (OEE2) which involved in drought stress tolerance response. Cultivars Giza 127, 130 and 134 showed highest tolerance response under drought stress. The antioxidant enzymes PAGE pattern of Peroxidase (POX), Sodium dismutase (SOD) and Ascorbate peroxidase (APX) for Barley cultivars under drought stress revealed a high activities for Giza 126, 127, 134, 136 and 2000 under −0.5 MPa osmotic stress by PEG in most of their isoforms. Based on similarity coefficient values the highest values were 1.0 with 100% similarly between tolerant cultivars Giza 130 and Giza 127. Similarly between the susceptible cultivars 125 and Giza 129 was 60%.These data confirmed by the growth parameters which we ranked as tolerant to drought stress.

show abstract

“…Therefore, the selection on yield components character can be started with the selection on drought resistance character. The drought resistance character can be identified based on the peroxidase isozyme (Nasr et al, 2009), since the increasing levels of drought stress can encourage the increase of peroxidase activities (Sharma and Dubey, 2005). It is caused by the peroxidase role in osmotic adjustment of cells, so the plant can be more resistant under drought stress (Omidi, 2010).…”

Section: Introductionmentioning

confidence: 99%

PEROXIDASE ISOZYME IDENTIFICATION OF SOME RICE GENOTYPES IN M1 GENERATION UNDER DROUGHT STRESS LEVEL OF -0.03 MPa

2014

View full text Add to dashboard Cite

The effort to fulfill the need of rice through the improvement of dryland productivity can be viewed as a more environmentally-friendly way. This research used 36 rice genotypes in M 1 generation that were grown hydrophonically under drought stress level of -0.03 MPa. The identifications were conducted based on peroxidase isozyme marker. The isozyme patterns in zymogram were binary-coded by visual scores for each genotype, based on the thickness and the number in the appearance of bands on certain migration distance. The migration distances were measured based on values of Rf. The similarity coefficients were calculated using Dice's coefficient that were used to construct dendrogram using the UPGMA employing the SAHN from the NTSYSpc 2.02. The results showed that the most resistant genotype under drought stress was R-4, and the genetic relationships among the genotypes were divided into two main groups, aromatic and nonaromatic group, in which some genotypes experienced the reduced levels of aromatic character (R-8 and R-9) and the drought resistance character (

show abstract

Molecular and Field Analysis of Some Barley Genotypes for Water Stress Tolerance

Cited by 6 publications

References 15 publications

Changes in Germination and Seedling Traits of Sesame under Simulated Drought

Changes in Germination and Seedling Traits of Sesame under Simulated Drought

Influence of PEG induced drought stress on molecular and biochemical constituents and seedling growth of Egyptian barley cultivars

PEROXIDASE ISOZYME IDENTIFICATION OF SOME RICE GENOTYPES IN M1 GENERATION UNDER DROUGHT STRESS LEVEL OF -0.03 MPa

Contact Info

Product

Resources

About