Molecular and functional characterization of CYP6BQ23, a cytochrome P450 conferring resistance to pyrethroids in European populations of pollen beetle, Meligethes aeneus

Zimmer, Christoph; Bass, Chris; Williamson, Martin S.; Kaussmann, Martin; Wölfel, Katharina; Gutbrod, Oliver; Nauen, Ralf

doi:10.1016/j.ibmb.2013.11.008

Cited by 91 publications

(89 citation statements)

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“…It has been reported that some P450 genes belonging to the CYP 4, 6, 9 and 12 families are involved in insecticide resistance [16]. For example, the cytochrome P450 gene CYP6BQ23 was recently shown to be the main factor causing pyrethroid resistance in M. aeneus across Europe through its hydroxylation of the alcohol moiety of pyrethroids to a less toxic hydroxy metabolite [22]. In B. tabaci and M. persicae , the cytochrome P450 (CYP6CM1 and CYP6CY3, respectively) over expression contributes to neonicotinoid insecticide resistance because these enzymes can catalyze a more rapid conversion of imidacloprid to its less active form, 5-hydroxy-imidacloprid [23, 24].…”

Section: Discussionmentioning

confidence: 99%

De novo transcriptome and expression profile analyses of the Asian corn borer (Ostrinia furnacalis) reveals relevant flubendiamide response genes

et al. 2017

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BackgroundThe Asian corn borer (ACB), Ostrinia furnacalis (Guenée), has become the most damaging insect pest of corn in Asia. However, the lack of genome or transcriptome information heavily hinders our further understanding of ACB in every aspect at a molecular level and on a genome-wide scale. Here, we used the Ion Torrent Personal Genome Machine (PGM) Sequencer to explore the ACB transcriptome and to identify relevant genes in response to flubendiamide, showing high selective activity against ACB.ResultsWe obtained 35,430 unigenes, with an average length of 716 bp, representing a dramatic expansion of existing cDNA sequences available for ACB. These sequences were annotated with Non-redundant Protein (Nr), Gene Ontology (GO), Clusters of Orthologous Groups (COG) and Kyoto Encyclopedia of Genes and Genomes (KEGG) to better understand their functions. A total of 31 cytochrome P450 monooxygenases (P450s), 27 carboxyl/cholinesterases (CCEs) and 19 glutathione S-transferases (GSTs) were manually curated to construct phylogenetic trees, and 25 unigenes encoding target proteins (acetylcholinesterase, nicotinic acetylcholine receptor, gamma-aminobutyric acid receptor, glutamate-gated chloride channel, voltage-gated sodium channel and ryanodine receptor) were identified. In addition, we compared and validated the differentially expressed unigenes upon flubendiamide treatment, revealing that the genes for detoxification enzymes (P450s and esterase), calcium signaling pathways and muscle control pathways (twitchin and tropomyosin), immunoglobulin (hemolin), chemosensory protein and heat shock protein 70 were significantly overexpressed in response to flubendiamide, while the genes for cuticular protein, protease and oxidoreductase showed much lower expression levels.ConclusionThe obtained transcriptome information provides large genomic resources available for further studies of ACB. The differentially expressed gene data will elucidate the molecular mechanisms of ACB in response to the novel diamide insecticide, flubendiamide. In particular, these findings will facilitate the identification of the genes involved in insecticide resistance and the development of new compounds to control the ACB.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-3431-6) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

De novo transcriptome and expression profile analyses of the Asian corn borer (Ostrinia furnacalis) reveals relevant flubendiamide response genes

et al. 2017

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“…Detoxification enzymes may play a role in resistance to some pyrethroids in T. absoluta (Martin, Ochou, Vaissayre, & Fournier, ; Stevenson et al., ; Yang et al., ; Zimmer et al., ). Silva et al.…”

Section: Discussionmentioning

confidence: 99%

Organophosphate and pyrethroid resistances in the tomato leaf miner Tuta absoluta (Lepidoptera: Gelechiidae) from Iran

Zibaee

Mahmood

Esmaeily

et al. 2017

J Applied Entomology

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The tomato leafminer, Tuta absoluta (Meyrich) (Lepidoptera: Gelechiidae), is a serious pest of tomato crops worldwide. The intensive use of chemical pesticides to control it has led to the selection of resistant populations. This study investigated the resistance of T. absoluta populations to pyrethroid and the organophosphate insecticides from ten regions of Iran. The resistance ratios at LC 50 for chlorpyrifos and diazinon varied among populations from 4.3 to 12 and from 1.4 to 9.0, respectively. The resistance ratios of the pyrethroids cypermethrin, deltamethrin and permethrin varied from 1.3 to 3.7, 2.7 to 13 and 1.2 to 4.3, respectively. Inclusion of synergists in toxicological bioassays and the variation observed in the activity of esterases, glutathione S-transferase and cytochrome P450-dependent monooxygenase suggest the existence of metabolically based resistance. Esterase and P450 biochemical assays were positively correlated with deltamethrin, and cypermethrin tolerance and diazinon tolerance correlated with esterase activity. The genes encoding the organophosphate and pyrethroid target sites acetylcholinesterase (ace1) and sodium channel (kdr) were partly sequenced. The genotyping revealed mutations in high frequencies in all populations leading to an A201S substitution in ace1 and three substitutions in the sodium channel gene L1014F, M918T, T929I. In summary, our results indicate the presence of organophosphate and pyrethroid resistance in Iranian T. absoluta populations with involvement of both detoxification enzymes and target site alterations. Most likely the populations of T. absoluta imported to Iran were resistant upon arrival. K E Y W O R D Sacetylcholinesterase, insecticide resistance, knock-down resistance, metabolic resistance, target site mutation, Tuta absoluta

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“…Several of about a hundred likely to be encoded by the H. armigera genome would be expected to have activity against fenvalerate, as some in vitro activity against pyrethroids has now been demonstrated for at least 15 members of four P450 families (CYP6, CYP9, CYP321 and CYP337) in various insects [12][13][14][15][16][17]. And higher expression in resistant strains has been demonstrated for 12 (from the CYP4, CYP6, CYP9, CYP332 and CYP337 families) of 30 H. armigera CYP genes tested [37].…”

Section: Discussionmentioning

confidence: 99%

“…In the case of H. armigera, the resistance is generally due to enhanced metabolism, and bioassays with the P450 inhibitor piperonyl butoxide (PBO) and the esterase inhibitor S,S,S-tributyl phosphorotrithioate (DEF) implicate both in the resistance but find that the relative contributions of the two classes of enzyme to resistance vary among populations [6][7][8][9][10][11]. Several individual P450s [12][13][14][15][16][17] and esterases [10,18,19] have been shown to metabolize pyrethroids and some, specifically CYP337B3, CYP6B7, CYP9A12 and CYP9A14 [20][21][22][23], and CCE001a, CCE001c, CCE001d and CCE001i [10,18,19], have also been found to be more highly expressed in resistant strains in at least one study. However quantitative trait loci (QTL) mapping has only so far directly associated one enzyme, CYP337B3, with resistance [14].…”

Section: Introductionmentioning

confidence: 99%

Variation in P450-mediated fenvalerate resistance levels is not correlated with CYP337B3 genotype in Chinese populations of Helicoverpa armigera

Han

Zhang

et al. 2015

Pesticide Biochemistry and Physiology

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Molecular and functional characterization of CYP6BQ23, a cytochrome P450 conferring resistance to pyrethroids in European populations of pollen beetle, Meligethes aeneus

Cited by 91 publications

References 41 publications

De novo transcriptome and expression profile analyses of the Asian corn borer (Ostrinia furnacalis) reveals relevant flubendiamide response genes

De novo transcriptome and expression profile analyses of the Asian corn borer (Ostrinia furnacalis) reveals relevant flubendiamide response genes

Organophosphate and pyrethroid resistances in the tomato leaf miner Tuta absoluta (Lepidoptera: Gelechiidae) from Iran

Variation in P450-mediated fenvalerate resistance levels is not correlated with CYP337B3 genotype in Chinese populations of Helicoverpa armigera

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