Molecular and functional characterization of myxobacteria isolated from soil in India

Abstract

This study reports the isolation of myxobacteria from soil collected from plains in north India. Based on the morphology and 16S rDNA sequence, the isolated myxobacteria were identified as Corallococcus sp., Pyxidicoccus sp., Myxococcus sp., Cystobacter sp. and Archangium sp. The myxobacteria were functionally characterized to assess their ability to produce antibacterial and anticancer metabolites. The isolates were found to be functionally versatile as they produced extracellular bioactive molecules that exhibited high frequency of activities against Bacillus cereus, Mycobacterium smegmatis, Enterobacter cloacae and Pseudomonas syringae. The strains also showed cytotoxic activity against the human cancer cell lines of liver, pancreas, prostrate, bone and cervix. These results indicate the importance of isolating diverse strains of myxobacteria from unexplored habitats to find novel bioactive compounds. Moreover, the bioactive molecules explored in this study are predominantly hydrophilic compounds, obviating the limitations of solubility-related aspect of drug discovery.

Multipotential Alkaline Protease From a Novel Pyxidicoccus sp. 252: Ecofriendly Replacement to Various Chemical Processes

“…The newly isolated strains of myxobacteria (S236, S244, S250, and S252) along with the previously reported strains were identified as protease producers based on the zone of clearance on skim milk agar medium at pH 11.0 ( Kumar et al, 2017 ). All the tested myxobacterial strains produced extracellular proteases.…”

“…Myxobacteria were isolated as described in Kumar et al (2017) . Briefly, soil samples were placed on E. coli paste spread on water calcium chloride clerigel (WCX clerigel; 0.1% CaCl 2 ⋅2H 2 O, 20 mM HEPES buffer, 0.8% clerigel, and 50 μg/ml cycloheximide; pH 7.2).…”

Biological ball filters regulate bacterial communities in marron ( Cherax cainii ) culture system

“…The bacteria Pajaroellobacter was identified from soil in earlier study (Kumar et al . ), but its functional role in water remediation has yet to be decoded. Finally, Corynebacterium growth was reported to be linked with higher temperature, whereas slow growth was recorded below 22°C (does not grow at 15°C).…”

“…Actinobacteria is an obligate chemoorganotrophs that thrives well on decaying organic matter (Shivlata and Satyanarayana 2015). A study on biodegradation of organic wastes in the ocean found significantly higher percentages of Actinobacteria (55Á9-76Á3%) along with Firmicutes (44Á1-44Á9%) than Proteobacteria (9Á2-15Á5%) (de Leo et al 2012). The positive role of Actinobacteria has been unequivocal for waste water treatment and heavy metal removal from the polluted sites (Wagner and ).…”

“…Haliscomonobacter was characterized from the aeration tanks of sewage and waste water treatment plants from paper industry and also reported to play a positive role in water remediation (Fourest et al 2004). The bacteria Pajaroellobacter was identified from soil in earlier study (Kumar et al 2017), but its functional role in water remediation has yet to be decoded. Finally, Corynebacterium growth was reported to be linked with higher temperature, whereas slow growth was recorded below 22°C (does not grow at 15°C).…”