Molecular and functional characterization of ovis aries IFN-epsilon

Guo, Yongli; Song, Zhifeng; Cheng, Xintong; Wang, Yujiao; Luo, Xiuxin; An, Ran; Wang, Junwei; Gao, Mingchun

doi:10.1016/j.molimm.2020.01.001

Cited by 12 publications

(10 citation statements)

References 23 publications

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“…In the CoV-infected pangolin skin, the interferon-speci c pathways were not enriched, and the expressions of many interferon pathway-related genes were undetectable or/and not signi cantly differentially expressed. In other placental mammals, IFNE provides the rst line of defence in the skin and mucosa-protected organs (e.g., lungs) and senses viral infection by triggering downstream genes involved in the interferon pathways [40][41][42][43][44][45][46] . Therefore, the IFNE-mediated pathways, including interferon-stimulated gene responses, are unlikely to be activated or up-regulated in the naturally IFNE-and IFIH1-de cient pangolins upon CoV infection 14 .…”

Section: Discussionmentioning

confidence: 99%

“…It has been reported that pangolins have unique immune characteristics 14,15 : both IFNE and IFIH1 (a cytoplasmic RNA sensor that helps initiates the innate immune response to viral infection) genes have been pseudogenised. IFNE plays an important antiviral role in epithelial cells [40][41][42][43][44][45][46] . Therefore, IFNE-de cient pangolin skin may be susceptible to CoV infection.…”

Section: Comparative Analysis Of Transcription In Dahu Skin and Humanmentioning

confidence: 99%

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Coronavirus in the Skin of the Pangolin: Implications for SARS-CoV-2 Transmission?

Deng

Tian

Belshaw

et al. 2021

Preprint

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COVID-19 has become a global pandemic caused by SARS-CoV-2 infection. Dermatologic manifestations are not uncommon in COVID-19 patients and the virus has been reported within some. However, the role of the virus and the host response in these cutaneous manifestations remain poorly studied. The pangolin coronavirus (pCoV) is a model for studying SARS-CoV-2 because of its genetic relatedness and similarity of disease characteristics. Here we report the presence of pCoV in pangolin skin manifestations and present an in-depth analysis of the transcriptional response to the infection. COVID-19-related pathways were up-regulated, but interferon epsilon (IFNE)-related pathways were not differentially regulated (consistent with the pseudogenisation of IFNE and IFIH1 in the pangolin). Endogenous retroviral genes tended to be down-regulated, indicating their importance in CoV infection. We believe our study provides further support for a potentially overlooked percutaneous transmission route of SARS-CoV-2 infection.

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Section: Discussionmentioning

confidence: 99%

Section: Comparative Analysis Of Transcription In Dahu Skin and Humanmentioning

confidence: 99%

Coronavirus in the Skin of the Pangolin: Implications for SARS-CoV-2 Transmission?

Deng

Tian

Belshaw

et al. 2021

Preprint

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“…It is presumed that ISG15 regulates endometrial receptivity in implantation, survival, growth and development of the conceptus (embryo and associated extraembryonic membranes; Johnson et al, 1999). There is a significant increase in ISG15 gene expression in the ovine uterus at 15 days of gestation (Guo et al, 2020). Expression of this gene has been found in parts of the stroma along the uteroplacental interface in gestation.…”

Section: Effect Of the Isg15 Gene On Embryonic Development In Sheepmentioning

confidence: 99%

Involvement of the Interferon Tau Gene in Maternal Recognition of Gestation in Sheep

Conde-Hinojosa

Gallegos‐Sánchez

Hernández

et al. 2021

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Objective: To describe the involvement of the interferon tau gene in the maternal recognition of pregnancy in sheep. Design/Methodology/Approach: A search and analysis of the scientific documents retrieved from the Web of Science and Scopus databases related to the functions of the interferon tau gene in the maternal recognition of pregnancy in sheep were conducted. Results: The interferon tau gene (IFNτ) participates in maternal recognition of pregnancy to avoid possible rejection of the embryo, and supports the secretion of progesterone involved in preparing the endometrium for implantation; it also inhibits myometrial motility to maintain pregnancy. IFNτ stimulates the transcription of so-called interferon-stimulated genes (ISGs), which are the effectors of cell-autonomous antiviral defense. One of the representative members of ISGs is the interferon 15-stimulated gene (ISG15) which regulates endometrial receptivity at implantation, as well as survival, growth and development of the conceptus. Study Limitations/Implications: Most embryonic losses occur between fertilization and maternal recognition of pregnancy. Understanding this issue is essential to understanding the possible causes of early pregnancy losses. Findings/Conclusions: Considerable progress has been made in the discovery of how the IFNτ and ISG15 genes act in maternal recognition of gestation in sheep

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“…Recombinant human IFNε can induce the production of an antiviral protein, MxA, to exhibit antiviral effects and inhibit human immunodeficiency virus replication (20). Recombinant IFN-ε from cattle, camels, dogs, and sheep has also shown significant antiviral activity (21)(22)(23)(24). Although IFN-α and IFN-β preparations have good antiviral effects, their long-term use may lead to side effects such as mental anxiety and depression.…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of the mink interferon-epsilon gene and its antiviral activity

Zhang

Zhang²,

Zhang³

et al. 2023

Front. Vet. Sci.

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The interferon (IFN) response is the first line of defense against viral invasion and thus plays a central role in the regulation of the immune response. IFN-epsilon (IFN-ε) is a newly discovered type I IFN that does not require viral induction, unlike other type I IFNs. IFN-ε is constitutively expressed in epithelial cells and plays an important role in mucosal immunity. In this study, we evaluated the biological activity of the mink-IFN (MiIFN)-ε gene in prokaryotic cells. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used to evaluate IFN-ε expression in different mink tissues. MiIFN-ε was highly expressed in brain, lung, tracheal, kidney, intestinal, bladder, ovarian, and testis tissues. There was no significant difference in MiIFN-ε expression between female and male minks, except in the reproductive system. Expression of the small ubiquitin-like modifier (SUMO3)-MiIFN-ε fusion gene was induced by isopropylβ-d-thiogalactoside, and MiIFN-ε was collected after SUMO-specific protease digestion. We tested the antiviral activity of MiIFN-ε against vesicular stomatitis virus (VSV) in epithelial cells of feline kidney 81 (F81). We used qRT-PCR to analyze the expression of several IFN-stimulated genes (ISGs), including ISG15, 2′-5′ oligoadenylate synthetase (2′-5′OAS1), and myxovirus resistance protein 1 (Mx1). Recombinant IFN-ε induced high ISG expression in F81 cells. Compared with those in the cell control group, expressions of ISG15, Mx1, and 2′-5′ OAS1 in the VSV-GFP control, IFN-ε, and MiIFN-ε-inhibited VSV-GFP groups were significantly increased. Compared with those in the VSV-GFP control group, expressions of ISG15 and 2′-5′ OAS1 in the IFN-ε and MiIFN-ε-inhibited VSV-GFP groups were significantly increased, and the differences were highly significant (p < 0.0001). IFN-ε played an indirect antiviral role. These findings lay the foundation for detailed investigation of IFN-ε in the future.

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Molecular and functional characterization of ovis aries IFN-epsilon

Cited by 12 publications

References 23 publications

Coronavirus in the Skin of the Pangolin: Implications for SARS-CoV-2 Transmission?

Coronavirus in the Skin of the Pangolin: Implications for SARS-CoV-2 Transmission?

Involvement of the Interferon Tau Gene in Maternal Recognition of Gestation in Sheep

Isolation and characterization of the mink interferon-epsilon gene and its antiviral activity

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