Molecular and genetic analysis of the yeast early meiotic recombination genes REC102 and REC107/MER2.

Cool, Marc; Malone, Robert E.

doi:10.1128/mcb.12.3.1248

Cited by 36 publications

(34 citation statements)

References 17 publications

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“…Cells with rec102 mutations also enter the first meiotic division at an earlier time, consistent with the view that REC102 is required for the normal timing of the reductional division Jiao et al, 1999). Northern analysis shows that REC102 is specifically transcribed during meiosis, and no transcripts can be detected in mitotic cells (Cool and Malone, 1992). REC102 expression is detectable about the same time as meiotic recombination starts, consistent with its role in the initiation of meiotic recombination (Cool and Malone, 1992).…”

Section: Introductionmentioning

confidence: 76%

“…Since S. paradoxus averages 80% nucleic acid identity, and S. pastorianus averages 65% identity with S. cerevisiae, comparisons of the REC102 promoter sequences among the three species might reveal which of the many candidate regulatory sequences (Cool and Malone, 1992) had been conserved. We cloned the REC102 homologues from these two species by screening genomic libraries of S. pastorianus and S. paradoxus (Nau et al, 1997) using a S. cerevisiae REC102 probe.…”

Section: Resultsmentioning

confidence: 99%

“…pCM208 contains the S. cerevisiae REC102 gene (Cool and Malone, 1992), and is used as the positive control for complementation tests. pRM263 is the vector pRS316 (Sikorski and Hieter, 1989) containing a 7.4 kb Sau3A/Sau3A fragment from S. paradoxus genomic DNA that hybridizes to REC102.…”

Section: Yeast Strains Media and Plasmidsmentioning

confidence: 99%

“…Our interest was initially motivated by the observation (Cool and Malone, 1992) that the promoter of REC102 contained multiple potential regulatory elements and might even have a regulatory element in the coding region. To determine which of the many possible regulatory sequences in the promoter region were actually important, we have used two different approaches.…”

Section: Introductionmentioning

confidence: 99%

“…REC102 is an early meiotic gene required for the initiation of meiotic recombination (Cool and Malone, 1992;Bhargava et al, 1992). Null rec102 mutations completely abolish all meiotic recombination, eliminate meiotic double-strand breaks, reduce spore viability to zero and diminish final sporulation values.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Phylogenetic footprinting reveals multiple regulatory elements involved in control of the meiotic recombination gene, REC102

Jiao

Nau

Cool

et al. 2001

Yeast

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REC102 is a meiosis-specific early exchange gene absolutely required for meiotic recombination in Saccharomyces cerevisiae. Sequence analysis of REC102 indicates that there are multiple potential regulatory elements in its promoter region, and a possible regulatory element in the coding region. This suggests that the regulation of REC102 may be complex and may include elements not yet reported in other meiotic genes. To identify potential cis-regulatory elements, phylogenetic footprinting analysis was used. REC102 homologues were cloned from other two Saccharomyces spp. and sequence comparison among the three species defined evolutionarily conserved elements. Deletion analysis demonstrated that the early meiotic gene regulatory element URS1 was necessary but not sufficient for proper regulation of REC102. Upstream elements, including the binding sites for Gcr1p, Yap1p, Rap1p and several novel conserved sequences, are also required for the normal regulation of REC102 as well as a Rap1p binding site located in the coding region. The data in this paper support the use of phylogenetic comparisions as a method for determining important sequences in complex promoters.

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Section: Introductionmentioning

confidence: 76%

Section: Resultsmentioning

confidence: 99%

Section: Yeast Strains Media and Plasmidsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Phylogenetic footprinting reveals multiple regulatory elements involved in control of the meiotic recombination gene, REC102

Jiao

Nau

Cool

et al. 2001

Yeast

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Characterization of REC104, a gene required for early meiotic recombination in the yeast Saccharomyces cerevisiae

Galbraith

Malone

1992

Dev. Genet.

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The REC104 gene was initially defined by mutations that rescued the inviability of a rad52 spo 13 haploid strain in meiosis. We have observed that rec104 mutant strains undergo essentially no induction of meiotic gene conversion, and we have not been able to detect any meiotic crossing over in such strains. The REC104 gene has no apparent role in mitosis, since mutations have no observable effect on growth, mitotic recombination, or DNA repair. The DNA sequence of REC104 reveals that it is a previously unknown gene with a coding region of 549-bp, and genetic mapping has localized the gene to chromosome VIII near FUR1. Expression of the REC104 gene is induced in meiosis, and it appears that the gene is not transcribed in mitotic cells. Possible roles for the REC104 gene product in meiosis are discussed.

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A nuclear mutation defective in mitochondrial recombination in yeast.

et al. 1995

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Homologous recombination (crossing over and gene conversion) is generally essential for heritage and DNA repair, and occasionally causes DNA aberrations, in nuclei of eukaryotes. However, little is known about the roles of homologous recombination in the inheritance and stability of mitochondrial DNA which is continuously damaged by reactive oxygen species, by‐products of respiration. Here, we report the first example of a nuclear recessive mutation which suggests an essential role for homologous recombination in the stable inheritance of mitochondrial DNA. For the detection of this class of mutants, we devised a novel procedure, ‘mitochondrial crossing in haploid’, which has enabled us to examine many mutant clones. Using this procedure, we examined mutants of Saccharomyces cerevisiae that showed an elevated UV induction of respiration‐deficient mutations. We obtained a mutant that was defective in both the omega‐intron homing and Endo.SceI‐induced homologous gene conversion. We found that the mutant cells are temperature sensitive in the maintenance of mitochondrial DNA. A tetrad analysis indicated that elevated UV induction of respiration‐deficient mutations, recombination deficiency and temperature sensitivity are all caused by a single nuclear mutation (mhr1) on chromosome XII. The pleiotropic characteristics of the mutant suggest an essential role for the MHR1 gene in DNA repair, recombination and the maintenance of DNA in mitochondria.

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Molecular and genetic analysis of the yeast early meiotic recombination genes REC102 and REC107/MER2.

Cited by 36 publications

References 17 publications

Phylogenetic footprinting reveals multiple regulatory elements involved in control of the meiotic recombination gene, REC102

Phylogenetic footprinting reveals multiple regulatory elements involved in control of the meiotic recombination gene, REC102

Characterization of REC104, a gene required for early meiotic recombination in the yeast Saccharomyces cerevisiae

A nuclear mutation defective in mitochondrial recombination in yeast.

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