Molecular and genetic characterization of the inheritance and expression of the synthetic porcine alpha-Lactalbumin transgene in maize (Zea mays L)

Abstract

Chapter 2. Inheritance and expression patterns of a synthetic 37 porcine a-Lactalbumin transgene in maize (Zea mays L.). Abstract Introduction Materials and Methods 41 Results 45 Discussion 51 References Chapter 3. Expression of the synthetic porcine a-Lactalbumin gene results in increased Lysine levels in transgenic maize kernels Abstract Introduction Materials and Methods Results Discussion References Chapter 4. The inheritance and expression patterns of the selectable marker gene BAR in maize (Zea maya L.). Abstract Introduction Materials and Methods Results and Discussion References Chapter 5. General Conclusions V 7b my Cko/g end mww Mwxto Aa&zA * Mean values are from three sibling kernels from each positive and negative class ± standard error. * = difference between positive and negative values is significant in a t-test at a = 0.05; ns = not significant.

Novel approaches to improving qualities of maize endosperm

“…The 27kD gamma zein promoter was chosen for these experiments because it is one of the highest expressing zeins, and has been used to drive high expression of proteins in maize endosperm [92,263,264] which could be useful in situations where high expression of a nutritional protein is desired, or where maize is to be used to produce biopharmaceudicals and similar products for extraction. However, there is some indication that the 27kDa gamma zein is an allergen in pigs [265], potentially making increased expression of the 27kDa gamma zein undesirable for some applications, such as biofortification.…”