Molecular and Immune Characteristics for Lung Adenocarcinoma Patients With ERLIN2 Overexpression

Abstract

BackgroundEndoplasmic reticulum lipid raft-associated protein 2 (ERLIN2) is protein contained in the membrane of the endoplasmic reticulum. In lung adenocarcinoma (LUAD), the molecular function of ERLIN2 and the correlation between ERLIN2 and tumor-infiltrating immune cells have been unclear. The aim of our study was to determine the role of ERLIN2 in LUAD development to provide a better understanding of the molecular pathogenesis of this disease and identify new therapeutic targets for its treatment.MethodsImmunohistochemistry, Western blotting, and real-time quantitative polymerase chain reaction were used to detect protein and mRNA levels of ERLIN2 in LUAD and adjacent normal tissues. Using the A549, H1299 cell line, ERLIN2-short hairpin RNA was applied to silence ERLIN2 to determine its role in LUAD cell proliferation and invasion. Based on mRNA expression of ERLIN2 from the Cancer Genome Atlas (TCGA) database, we identified ERLIN2-related protein-coding genes and analyzed the Kyoto Encyclopedia of Genes and Genomes pathway to explore its potential biological functions and determined the correlation between ERLIN2 and tumor-infiltrating immune cells.ResultsERLIN2 was abnormally expressed in a variety of tumor tissues and is highly expressed in LUAD. This overexpression was associated with histological grade (P = 0.044), TNM stage (P = 0.01), and lymph node metastasis (P = 0.038). Patient overall survival was poorer with ERLIN2 overexpression. Downregulation of ERLIN2 inhibited LUAD cell proliferation and invasion in vitro. Based on mRNA expression of ERLIN2 from the TCGA database, 13 ERLIN2-related genes and 10 pathways were identified and showed a correlation between ERLIN2 and naive B cells and neutrophils.ConclusionERLIN2 could serve as a potential diagnostic and prognostic biomarker for LUAD and has demonstrated to be correlated with immune infiltrates, which suggests that it may represent a new therapeutic target for LUAD.

Construction of a Nomogram for Predicting Survival in Elderly Patients With Lung Adenocarcinoma: A Retrospective Cohort Study

“…The histologic grades of tumors were mostly II and III, while the AJCC stages were mostly advanced with distant metastases. A previous study ( 2 , 20 ) similarly found that 76% of lung cancer patients were old and white, more than 90% of tumors were in the upper or lower lobes of the lung, and 80–90% of AC patients among the known pathological types had poorly or moderately differentiated histologic grades ( 25 , 26 ). These characteristics are similar to those in our study.…”

A Deep Transcriptome Meta-Analysis Reveals Sex-based Molecular Differences in Multiple Sclerosis

“…described as an antisense transcript of ERLIN2, a gene associated with juvenile primary lateral sclerosis and immune cells infiltration [53][54][55]. We found 11 and 15 genes significantly altered in the IDF and IDM comparisons, respectively; however, none displayed commonality (Figure 6, Table 7).…”

“…Importantly, ERLIN2 mutations lead to abnormal splicing and nonsense-mediated mRNA decay, promoting motor neurodegeneration and juvenile amyotrophic lateral sclerosis [55]. Additionally, ERLIN2 has been related to the infiltration of immune cells in the tumor microenvironment [54]. Therefore, LOC102723701 may represent an exciting and novel target in MS for future research.…”

Role of cuproptosis-related gene in lung adenocarcinoma

“…The abundances of B cells, plasma cells, resting memory T cells CD4, resting NK cells, monocytes, M2 macrophages, resting DCs, resting mast cells, and neutrophils were substantially different between high and low riskscore group. Ample evidence has shown that the infiltration of B cells, especially naive B cells, memory B cells, and plasma cells is associated with a good prognosis of LUAD ( 38 ). In contrast, we found the high-risk subgroup had a poor prognosis while high plasma cell expression.…”