Molecular and immunohistochemical studies do not support a role for papillomaviruses in canine oral squamous cell carcinoma development

Munday, John S.; French, Adrienne F.; Harvey, Colin E.

doi:10.1016/j.tvjl.2015.03.002

Cited by 21 publications

(19 citation statements)

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“…Furthermore, a novel PV type (CPV-17) was detected in a dog that developed multiple oral SCCs [ 21 ]. Additional studies by Munday and colleagues did not detect PV DNA in any of 23 subungual [ 31 ] and 28 oral SCC [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

“…One study reported an intense diffuse p16 immunostaining in a multiple canine oral SCC with papillomaviral DNA and cytopathology [ 21 ]. In the same year, a moderate to strong immunostaining for p16 was detected in 4 out of 28 (14.3%) oral SCCs; however, the absence of amplifiable PV DNA in those cases suggested that the increased p16 was unlikely due to PV infection [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

“…Immunohistochemistry for p16 was performed using a commercial mouse anti-human monoclonal antibody (BD Biosciences, San Jose, CA, USA) whose specificity for canine p16 had been previously validated [ 21 , 26 ].…”

Section: Methodsmentioning

confidence: 99%

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p16 Immunostaining of Canine Squamous Cell Carcinomas Is Not Associated with Papillomaviral DNA

et al. 2016

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While papillomavirus (PVs) are an established cause of human cancer, few reports have supported a relationship between PV and canine squamous cell carcinomas (SCCs). Human oncogenic PVs lead to an increased expression of the p16 tumor suppressor protein, and the latter can be demonstrated immunohistochemically to support a likely causal relationship between tumor and PV infection. In the present study, archive samples of canine SCC from different anatomical locations were tested by polymerase chain reaction for the presence of PV DNA and by p16 immunohistochemistry. The aims were to investigate the relationship between p16 expression and presence of PV DNA, in order to assess the utility of p16 overexpression as a biomarker of PV infection in canine SCC. A total of 52 SCCs were included. Nine cases (17.3%) showed moderate p16 immunoreactivity, with no association with tumor degree of differentiation, histotype or mitotic activity. The canPVf/FAP64 primers amplified Canis familiaris PV-1 DNA from 3 out of 52 tumors (5.8%), one cutaneous, one oral and one tonsillar SCC. There was no association between PV presence and p16 immunostaining. These results do not support a significant role of PVs in the development of canine SCCs. Additionally, PV infection was apparently not the cause of the p16 immunostaining observed in a subset of canine SCCs. A better awareness of p16 level of expression and cellular function in canine cancer may help to define its diagnostic and prognostic role.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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p16 Immunostaining of Canine Squamous Cell Carcinomas Is Not Associated with Papillomaviral DNA

et al. 2016

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“…CPV 1 usually affects young dogs and causes papillomatosis and benign mucosal lesions in the oral cavity with occasional outbreaks in groups [24,25]. Several retrospective studies based on molecular or serological survey evidence have declared that CPV 1 is unlikely to bring about malignant changes [49][50][51]. Controversially, accumulative studies have revealed the identification of CPV 1 in SCCs [26,27].…”

Section: Discussionmentioning

confidence: 99%

The Detection and Association of Canine Papillomavirus with Benign and Malignant Skin Lesions in Dogs

Chang

Chen

Haga

et al. 2020

Viruses

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Papillomavirus (PV) mainly infects the squamous epithelium and may potentially lead to benign or even malignant cutaneous lesions. However, the malignant transforming ability has been identified in several types of PVs. In humans, papillomavirus (HPV) type 16 and 18 are the most prevalent causative agents of cervical cancer. Therefore, vaccines are being developed to protect against these types. For dogs, there have been limited investigations into the association of different canine papillomavirus (CPV) genotypes with malignant lesions. Understanding the high-risk CPV genotype(s) responsible for these malignant lesions would contribute to the development of interventions for preventing CPV-induced carcinomas. In the present study, a retrospective cohort of 102 pathologically confirmed papillomas and 212 squamous cell carcinomas (SCCs) were included. The viral genome and antigens in the formalin-fixed paraffin-embedded (FFPE) tissues were detected using PCR targeting pan PV E1 and COPV L1 genes and by immunohistochemistry staining (IHC), respectively. PVs were successfully detected from 11 FFPE cutaneous tissues and four oral tissues using pan PV E1- and COPV L1-based PCR, respectively. After sequencing, CPV 1, CPV 2, and CPV 6 were detected in the benign lesions using PCR and were confirmed through IHC. While CPV 9 and CPV 15 were first detected in the SCCs of dogs, CPV 16 was most often detected in SCC specimens. The association and confirmative demonstration of viral genes and intralesional antigens of CPV 9, CPV 15, and CPV 16 in SCCs highlight the potential risk of these genotypes of CPVs in malignant transformation.

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“…CPV1, long known as the canine oral papillomavirus (CoPV), is associated with oral papillomatosis (Porcellato et al., ; Sancak et al., ), together with CPV13 (Lange et al., ), and possibly, a subset of conjunctival lesions (Brandes et al., ). However, most oral papillomas do not progress to invasive cancers and papillomaviral DNA is rarely detected in canine oral squamous cell carcinomas (SCC) (Porcellato et al., ; Munday et al., ). Still, while oral SCC appear to be rarely caused by papillomavirus infection, one dog was reported to develop multiple oral SCCs associated with infection by the recently detected CPV‐17 (Munday et al., , ).…”

Section: Papillomavirus In Companion Animals: Which Viruses Cause Whimentioning

confidence: 99%

An Update on Canine, Feline and Bovine Papillomaviruses

Costa

Peleteiro

Pires

et al. 2016

Transbound Emerg Dis

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Over recent years, a growing number of papillomaviruses have been identified, which cause a wide range of lesions in domestic and wild animals. Papillomavirus-induced lesions may have a great impact on animal health, and some diseases observed in farm animals are associated with significant economic losses. This concise review brings together recent advancements on animal papillomavirus research, providing the scientific community and veterinary practitioners with an update on this rapidly evolving field. Among others, bovine, canine and feline papillomaviruses (BPV, CPV and FcaPV) are most extensively discussed, in view of the recent discovery of new viral types and their worldwide importance for animal health. Feline papillomaviruses 2 is an emerging, highly prevalent pathogen in domestic cats, associated with a subset of malignant skin lesions. Aspects related to cross-species infection by BPV and its environmental co-factors are also addressed. Animal papillomaviruses are also fascinating models for studying molecular and cell biology and have recently inspired some major breakthroughs. Overall, it is clear that additional, international and systematic efforts are needed to clarify which lesions are caused by which viral types and to develop experimental models for studying animal papillomavirus.

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Molecular and immunohistochemical studies do not support a role for papillomaviruses in canine oral squamous cell carcinoma development

Cited by 21 publications

References 10 publications

p16 Immunostaining of Canine Squamous Cell Carcinomas Is Not Associated with Papillomaviral DNA

p16 Immunostaining of Canine Squamous Cell Carcinomas Is Not Associated with Papillomaviral DNA

The Detection and Association of Canine Papillomavirus with Benign and Malignant Skin Lesions in Dogs

An Update on Canine, Feline and Bovine Papillomaviruses

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