Molecular and immunological characterization of <i>Mycobacterium avium</i> 65 kDa heat shock protein (Hsp65)

Nagabhushanam, Vijaya; Praszkier, Judyta; Cheers, Christina

doi:10.1046/j.1440-1711.2001.01032.x

Cited by 9 publications

(9 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This hypothesis is plausible as hsp65 is highly conserved and present in all Mycobacterium spp. despite not being very immunogenic in M. avium [33]. The significant increase in IgG1 and IgG2a anti‐hsp65 antibodies observed after pVAXhsp65 immunization in M. avium ‐primed mice supports this possibility.…”

Section: Discussionmentioning

confidence: 93%

“…However, in work by Nagabhushanam et al. [33], hsp65 was not considered a dominant T cell antigen in M. avium infections.…”

Section: Discussionmentioning

confidence: 99%

“…The lack of anti-hsp65 antibodies in mice injected with heat-killed M. avium was a little unexpected because hsp65 has been described as an immunodominant antigen in mycobacterial infections [32]. However, in work by Nagabhushanam et al [33], hsp65 was not considered a dominant T cell antigen in M. avium infections.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Exposure to Mycobacterium avium Decreases the Protective Effect of the DNA Vaccine pVAXhsp65 Against Mycobacterium tuberculosis‐Induced Inflammation of the Pulmonary Parenchyma

et al. 2011

View full text Add to dashboard Cite

This work investigated the effect of previous Mycobacterium avium exposure on the protective ability of the DNA vaccine pVAXhsp65 against inflammation in the pulmonary parenchyma. BALB/c mice were presensitized with heat‐killed M. avium and then immunized with three doses of pVAXhsp65 prior to challenge with Mycobacterium tuberculosis. M. avium sensitization induced high levels of spontaneous IL‐5 production that were concomitant with a positive delayed‐type hypersensitivity reaction; antigen‐specific IFN‐γ production was also observed upon splenocyte stimulation. Prior exposure to M. avium resulted in altered cytokine and antibody production induced by immunization with pVAXhsp65; instead of a Th1 response, vaccinated mice previously exposed to M. avium developed a strong Th2 response. This switch to a Th2 response coincided with the loss of the anti‐inflammatory effect of pVAXhsp65 vaccination previously observed in the pulmonary parenchyma of mice infected with M. tuberculosis. These results suggest that exposure to environmental mycobacteria can modulate immune responses induced by mycobacterial vaccines other than bacillus Calmette–Guérin.

show abstract

Section: Discussionmentioning

confidence: 93%

“…However, in work by Nagabhushanam et al. [33], hsp65 was not considered a dominant T cell antigen in M. avium infections.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Exposure to Mycobacterium avium Decreases the Protective Effect of the DNA Vaccine pVAXhsp65 Against Mycobacterium tuberculosis‐Induced Inflammation of the Pulmonary Parenchyma

et al. 2011

View full text Add to dashboard Cite

show abstract

“…However, a recombinant HspX protein did not generate a CMI recall response when expressed in cell culture from 10 infected cows (Bannantine & Stabel 2000). In another study, HspX and Hsp65 (Koets et al 1999;Nagabhushanam et al 2001) induced low CMI response in both cattle (Koets et al 1999) and mice (Nagabhushanam et al 2001). …”

Section: Map Antigen Candidates For Diagnosticsmentioning

confidence: 94%

Trends and advances in the diagnosis and control of paratuberculosis in domestic livestock

Chaubey

Gupta

et al. 2016

Veterinary Quarterly

View full text Add to dashboard Cite

Paratuberculosis (pTB) is a chronic granulomatous enteritis caused by Mycobacterium avium subsp. paratuberculosis (MAP) in a wide variety of domestic and wild animals. Control of pTB is difficult due to the lack of sensitive, efficacious and cost-effective diagnostics and marker vaccines. Microscopy, culture, and PCR have been used for the screening of MAP infection in animals for quite a long time. Besides, giving variable sensitivity and specificity, these tests have not been considered ideal for large-scale screening of domestic livestock. Serological tests like ELISA easily detects anti-MAP antibodies. However, it cannot differentiate between the vaccinated and infected animals. Nanotechnology-based diagnostic tests are underway to improve the sensitivity and specificity. Newer generation diagnostic tests based on recombinant MAP secretory proteins would open new paradigm for the differentiation between infected and vaccinated animals and for early detection of the infection. Due to higher seroreactivity of secretory proteins vis-a-vis cellular proteins, the secretory proteins may be used as marker vaccine, which may aid in the control of pTB infection in animals. Secretory proteins can be potentially used to develop future diagnostics, surveillance and monitoring of the disease progression in animals and the marker vaccine for the control and eradication of pTB.

show abstract

“…Mycobacterium avium lysate was prepared as previously described 18 . Recombinant Hsp65 (rHsp65) was prepared from lysates of E. coli transformed with a recombinant plasmid (p2GEX‐2T hsp65) expressing the M. avium Hsp65 as a GST‐fusion protein 19 …”

Section: Methodsmentioning

confidence: 99%

Identification of an I‐A^d restricted peptide on the 65‐kilodalton heat shock protein of Mycobacterium avium

et al. 2002

Self Cite

View full text Add to dashboard Cite

The 65 kilodalton heat shock protein (Hsp65) from mycobacterial species elicits immune responses and in some cases protective immunity. Here we have used a DNA sublibrary approach to identify antigenic fragments of Mycobacterium avium Hsp65 and a synthetic peptide approach to delineate CD4 + T cell determinants. A panel of Hsp65 reactive CD4 + T cell clones was established from lymph node cells obtained from BALB/c mice immunized with recombinant Hsp65. The clones were tested for proliferative reactivity against the products of the DNA sublibrary of the hsp65 gene. A T cell epitope, restricted by the I-A d molecule, was identified within the C-terminal region of Hsp65 and the minimal epitope (amino acid residues 489-503) delineated using overlapping peptides spanning the C-terminal fragment. Additionally, the CD4 + T cell clone recognizing this epitope also responded to native Hsp65 present in M. avium lysates by both proliferation and cytokine production, indicating that the epitope was present and processed similarly both in the native and the recombinant forms of Hsp65. This sequence identified in BALB/c mice (Hsp65 489-503) is identical in other mycobacteria, notably M. tuberculosis , M. bovis and M. leprae , suggesting the epitope may have wider application in murine models of other mycobacterial infections.

show abstract

Molecular and immunological characterization of Mycobacterium avium 65 kDa heat shock protein (Hsp65)

Cited by 9 publications

References 42 publications

Exposure to Mycobacterium avium Decreases the Protective Effect of the DNA Vaccine pVAXhsp65 Against Mycobacterium tuberculosis‐Induced Inflammation of the Pulmonary Parenchyma

Exposure to Mycobacterium avium Decreases the Protective Effect of the DNA Vaccine pVAXhsp65 Against Mycobacterium tuberculosis‐Induced Inflammation of the Pulmonary Parenchyma

Trends and advances in the diagnosis and control of paratuberculosis in domestic livestock

Identification of an I‐A^d restricted peptide on the 65‐kilodalton heat shock protein of Mycobacterium avium

Contact Info

Product

Resources

About

Molecular and immunological characterization of Mycobacterium avium 65 kDa heat shock protein (Hsp65)

Cited by 9 publications

References 42 publications

Exposure to Mycobacterium avium Decreases the Protective Effect of the DNA Vaccine pVAXhsp65 Against Mycobacterium tuberculosis‐Induced Inflammation of the Pulmonary Parenchyma

Exposure to Mycobacterium avium Decreases the Protective Effect of the DNA Vaccine pVAXhsp65 Against Mycobacterium tuberculosis‐Induced Inflammation of the Pulmonary Parenchyma

Trends and advances in the diagnosis and control of paratuberculosis in domestic livestock

Identification of an I‐Ad restricted peptide on the 65‐kilodalton heat shock protein of Mycobacterium avium

Contact Info

Product

Resources

About

Identification of an I‐A^d restricted peptide on the 65‐kilodalton heat shock protein of Mycobacterium avium