Molecular and in silico evidences explain the anti-inflammatory effect of Trachyspermum ammi essential oil in lipopolysaccharide induced macrophages

Bahuguna, Ashutosh; Ramalingam, Srinivasan; Arumugam, Aruna; Kim, Myung‐Hee

doi:10.1016/j.procbio.2020.06.006

Cited by 7 publications

(3 citation statements)

References 29 publications

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“…Our results are in accordance with previously published data regarding the influence of the investigated Apiaceae EOs and their main compounds on pro-inflammatory cytokine production. Thus, ajowan EO was shown to possess anti-inflammatory effects as assessed by in vitro studies on murine macrophage cells, by inhibiting cyclooxygenase-2, inducible nitric oxide synthase, and heme oxygenase-1 [71]. Coriander EO exhibited anti-inflammatory effects in an in vivo model of colitis as determined by histo-pathologic evaluation and myeloperoxidase activity [72]; meanwhile, its main constituent linalool was found to inhibit the production of pro-inflammatory cytokines (e.g., TNF-α, IL-1β, IL-6, IL-8) in both in vitro and in vivo settings [73,74].…”

Section: Discussionmentioning

confidence: 99%

Apiaceae Essential Oils: Boosters of Terbinafine Activity against Dermatophytes and Potent Anti-Inflammatory Effectors

Trifan

Luca

Bostănaru

et al. 2021

Plants

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Dermatophyte infections represent an important public health concern, affecting up to 25% of the world’s population. Trichophyton rubrum and T. mentagrophytes are the predominant dermatophytes in cutaneous infections, with a prevalence accounting for 70% of dermatophytoses. Although terbinafine represents the preferred treatment, its clinical use is hampered by side effects, drug–drug interactions, and the emergence of resistant clinical isolates. Combination therapy, associating terbinafine and essential oils (EOs), represents a promising strategy in the treatment of dermatophytosis. In this study, we screened the potential of selected Apiaceae EOs (ajowan, coriander, caraway, and anise) to improve the antifungal activity of terbinafine against T. rubrum ATCC 28188 and T. mentagrophytes ATCC 9533. The chemical profile of EOs was analyzed by gas chromatography. The minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) of EOs/main compounds were determined according to EUCAST-AFST guidelines, with minor modifications. The checkerboard microtiter method was used to identify putative synergistic combinations of EOs/main constituents with terbinafine. The influence of EOs on the viability and pro-inflammatory cytokine production (IL-1β, IL-8 and TNF-α) was determined using an ex vivo human neutrophils model. The binary associations of tested EOs with terbinafine were found to be synergistic against T. rubrum, with FICI values of 0.26–0.31. At the tested concentrations (6.25–25 mg/L), EOs did not exert cytotoxic effects towards human neutrophils. Anise EO was the most potent inhibitor of IL-1β release (46.49% inhibition at 25 mg/L), while coriander EO displayed the highest inhibition towards IL-8 and TNF-α production (54.15% and 54.91%, respectively). In conclusion, the synergistic combinations of terbinafine and investigated Apiaceae EOs could be a starting point in the development of novel topical therapies against T. rubrum-related dermatophytosis.

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Section: Discussionmentioning

confidence: 99%

Apiaceae Essential Oils: Boosters of Terbinafine Activity against Dermatophytes and Potent Anti-Inflammatory Effectors

Trifan

Luca

Bostănaru

et al. 2021

Plants

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“…The anti-inflammatory activity of S. tenuifolia EO was evaluated using a NO inhibitory assay (Bahuguna et al, 2020;Hsieh et al, 2007).…”

Section: No Inhibitory Assaymentioning

confidence: 99%

Spectrum–effect relationship between GC‐QTOF‐MS fingerprint and antioxidant, anti‐inflammatory activities of Schizonepeta tenuifolia essential oil

Bai

Liu

Zhang

et al. 2021

Biomedical Chromatography

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Schizonepeta tenuifolia (Benth.) Briq, a traditional Chinese medicine, is an annual herbaceous plant that is widely distributed in China, Japan, and Korea. The essential oil (EO) of S. tenuifolia has antioxidant and anti-inflammatory properties. However, the components contributing to its antioxidant and anti-inflammatory activities remain unclear. This study was aimed at investigating the spectrum-effect relationship between GC-MS fingerprint and the antioxidant and anti-inflammatory effects of S. tenuifolia EO. Here, the fingerprints of EO from 10 batches of S. tenuifolia from various sources were established using GC-MS, and the antioxidant and antiinflammatory bioactivities were evaluated using 2,2-diphenyl-1-picrylhydrazyl and nitric oxide inhibitory assays, respectively. Finally, 13 common peaks were identified from 10 batches of S. tenuifolia by searching against the standard mass spectra in NIST 14 and comparing the literature retention index. The different sources of S. tenuifolia EO exhibit mild antioxidant activities and significant anti-inflammatory effects. In particular, menthone (peak 3), isomenthone (peak 4), pulegone (peak 7), piperitone (peak 8), and β-caryophyllene (peak 11) might be the dominant constituents responsible for the antioxidant and anti-inflammatory activities of S. tenuifolia EO. This method may provide a time-saving, convenient way to screen the potential effective components of S. tenuifolia EO.

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“…790 mg/kg(Perazzo et al 2003), thymol and carvacrol bioactive component i.e. 980 mg/kg and 810 mg/kg(Bahuguna et al 2020) recommending nanoformulated HAEO as safer next generation green preservative.…”

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confidence: 99%

Chemically characterized nanoencapsulated Homalomena aromatica Schott. essential oil as green preservative against fungal and aflatoxin B1 contamination of stored spices based on in vitro and in situ efficacy and favorable safety profile on mice

Tiwari

Upadhyay

Singh

et al. 2021

Environ Sci Pollut Res

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Present study deals with the efficacy of nanoencapsulated Homalomena aromatica essential oil (HAEO) as a potent green preservative against toxigenic Aspergillus flavus strain (AF-LHP-NS 7), AFB1 and free radical mediated deterioration of stored spices. GC-MS analysis revealed linalool (68.51%) as the major component of HAEO. HAEO was encapsulated into chitosan nanomatrix (CS-HAEO-Ne) and characterized through SEM, FTIR and XRD. CS-HAEO-Ne completely inhibited A. flavus growth and AFB1 biosynthesis at 1.25 μL/mL and 1.0 μL/mL, respectively in comparison to unencapsulated HAEO (1.75 μL/mL and 1.25 μL/mL respectively). CS-HAEO-Ne exhibited superior antioxidant efficacy (IC50 (DPPH) = 4.5 μL/mL) over unencapsulated HAEO (IC50 (DPPH) = 15.9 μL/mL). Further, CS-HAEO-Ne caused significant reduction in ergosterol content in treated A. flavus and provoked leakage of cellular ions (Ca +2 , Mg +2 and K + ) as well as 260 nm and 280 nm absorbing materials. Depletion of methylglyoxal level in treated A. flavus cells deals with the novel antiaflatoxigenic efficacy of CS-HAEO-Ne. CS-HAEO-Ne depicted excellent in situ efficacy by inhibiting mold attack and AFB1 contamination, mineral preservation and acceptable sensorial profile. Moreover, broad safety paradigm (LD50 value = 8006.84 µL/kg) of CS-HAEO-Ne also suggest it as novel green preservative to enhance shelf life of stored spices.

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Molecular and in silico evidences explain the anti-inflammatory effect of Trachyspermum ammi essential oil in lipopolysaccharide induced macrophages

Cited by 7 publications

References 29 publications

Apiaceae Essential Oils: Boosters of Terbinafine Activity against Dermatophytes and Potent Anti-Inflammatory Effectors

Apiaceae Essential Oils: Boosters of Terbinafine Activity against Dermatophytes and Potent Anti-Inflammatory Effectors

Spectrum–effect relationship between GC‐QTOF‐MS fingerprint and antioxidant, anti‐inflammatory activities of Schizonepeta tenuifolia essential oil

Chemically characterized nanoencapsulated Homalomena aromatica Schott. essential oil as green preservative against fungal and aflatoxin B1 contamination of stored spices based on in vitro and in situ efficacy and favorable safety profile on mice

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