Molecular AND logic gate for multiple single-nucleotide mutations detection based on CRISPR/Cas9n system-trigged signal amplification

Chang, Weidan; Liu, Weipeng; Shen, Haoran; Chen, Sisi; Liao, Peizhen; Liu, Yingju

doi:10.1016/j.aca.2020.03.058

Cited by 17 publications

(8 citation statements)

References 50 publications

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“…[3][4][5] Furthermore, CRISPR/cas system also holds great potential in the biological detection field. [6][7][8] Cas9 is one of the most widely applied cas protein and has been confirmed to possess a certain ability for biosensing. [9][10][11] Although Cas9 has excellent selectivity, Cas9 recognizes doublestranded (ds-) DNA with a unique protospacer adjacent motif (PAM) in a 1 : 1 ratio and generates a break with blunt ends, which limit its exploitability in biosensing.…”

mentioning

confidence: 99%

Cascade CRISPR/cas enables amplification-free microRNA sensing with fM-sensitivity and single-base-specificity

Yang

Huang

et al. 2021

Chem. Commun.

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mentioning

confidence: 99%

Cascade CRISPR/cas enables amplification-free microRNA sensing with fM-sensitivity and single-base-specificity

Yang

Huang

et al. 2021

Chem. Commun.

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“…With this platform, the detection of MDRAB could be achieved within 2 h with a LOD as low as 50 CFU mL −1 . [279] In addition, CRISPR/Cas systems-based bio/nanosensors have also been applied in the detection of fungus, such as Phytophthora infestans, [65,281] Candida, and Aspergillus (Table 2). [282]…”

Section: Othersmentioning

confidence: 99%

CRISPR/Cas Systems‐Inspired Nano/Biosensors for Detecting Infectious Viruses and Pathogenic Bacteria

et al. 2022

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Infectious pathogens cause severe human illnesses and great deaths per year worldwide. Rapid, sensitive, and accurate detection of pathogens is of great importance for preventing infectious diseases caused by pathogens and optimizing medical healthcare systems. Inspired by a microbial defense system (i.e., CRISPR/ CRISPR-associated proteins (Cas) system, an adaptive immune system for protecting microorganisms from being attacked by invading species), a great many new biosensors have been successfully developed and widely applied in the detection of infectious viruses and pathogenic bacteria. Moreover, advanced nanotechnologies have also been integrated into these biosensors to improve their detection stability, sensitivity, and accuracy. In this review, the recent advance in CRISPR/Cas systems-based nano/biosensors and their applications in the detection of infectious viruses and pathogenic bacteria are comprehensively reviewed. First of all, the categories and working principles of CRISPR/Cas systems for establishing the nano/biosensors are simply introduced. Then, the design and construction of CRISPR/Cas systems-based nano/biosensors are comprehensively discussed. In the end, attentions are focused on the applications of CRISPR/Cas systems-based nano/biosensors in the detection of infectious viruses and pathogenic bacteria. Impressively, the remaining opportunities and challenges for the further design and development of CRISPR/Cas system-based nano/biosensors and their promising applications are proposed.

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“…This work was later continued by Amir et al to display such nanorobot interactions to achieve even more complex operations, even inside living cockroaches [63]. Other DNA-based studies have used a CRISPR system to behave as an AND gate [83] or incorporated into cell membranes to detect dangerous cells by their surfaces [84]. Table 2 summarizes information from the studies presented in this section.…”

Section: Logic Gates Using Fimentioning

confidence: 99%

Fluorescence for biological logic gates

Barnoy

Popovtzer

Fixler

2020

Journal of Biophotonics

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Biological logic gates are smart probes able to respond to biological conditions in behaviors similar to computer logic gates, and they pose a promising challenge for modern medicine. Researchers are creating many kinds of smart nanostructures that can respond to various biological parameters such as pH, ion presence, and enzyme activity. Each of these conditions alone might be interesting in a biological sense, but their interactions are what define specific disease conditions. Researchers over the past few decades have developed a plethora of stimuli‐responsive nanodevices, from activatable fluorescent probes to DNA origami nanomachines, many explicitly defining logic operations. Whereas many smart configurations have been explored, in this review we focus on logic operations actuated through fluorescent signals. We discuss the applicability of fluorescence as a means of logic gate implementation, and consider the use of both fluorescence intensity as well as fluorescence lifetime.

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Molecular AND logic gate for multiple single-nucleotide mutations detection based on CRISPR/Cas9n system-trigged signal amplification

Cited by 17 publications

References 50 publications

Cascade CRISPR/cas enables amplification-free microRNA sensing with fM-sensitivity and single-base-specificity

Cascade CRISPR/cas enables amplification-free microRNA sensing with fM-sensitivity and single-base-specificity

CRISPR/Cas Systems‐Inspired Nano/Biosensors for Detecting Infectious Viruses and Pathogenic Bacteria

Fluorescence for biological logic gates

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