Molecular and morphological characterisation of species within the Xiphinema americanum-group (Dorylaimida: Longidoridae) from the central valley of Chile

Meza, Pablo; Aballay, Erwin; Hinrichsen, Patricio

doi:10.1163/138855410x518498

Cited by 9 publications

(6 citation statements)

References 6 publications

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“…Sequences revealing high similarity to those obtained here were included in the phylogenetic analyses of both ribosomal and mitochondrial gene regions (Neilson et al 2004, Oliveira et al 2004, He et al 2005b, Gozel et al 2006, Holterman et al 2006; Lazarova et al 2006, Kumari et al 2009, Gutiérrez-Gutiérrez et al 2010, Kumari et al 2010a, Kumari et al 2010b, De Luca and Agostinelli 2011, Gutiérrez-Gutiérrez et al 2011a, Gutiérrez-Gutiérrez et al 2011b, Meza et al 2011, Sakai et al 2011, Gutiérrez-Gutiérrez et al 2012, Kumari and Subbotin 2012, Sakai et al 2012, Kumari and Cesare 2013, Tzortzakakis et al 2014, Getaneh et al 2015, etc). Sequence numbers are presented in the trees.…”

Section: Methodsmentioning

confidence: 95%

Morphological and molecular characterisation, and phylogenetic position of X. browni sp. n., X. penevi sp. n. and two known species of Xiphinema americanum-group (Nematoda, Longidoridae)

Lazarova¹,

Peneva²,

Kumari³

2016

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Using ribosomal (18S, ITS1, ITS2, D2-D3 expansion segments of 28S rDNA) and mitochondrial (partial cox1 and nad4) DNA markers in a study of several populations of Xiphinema americanum-group from Europe and Morocco, two cryptic species Xiphinema browni sp. n. (formerly reported as Xiphinema pachtaicum) and Xiphinema penevi sp. n. were revealed. The species are described, illustrated and their phylogenetic relationships discussed. The first species is most similar to Xiphinema parasimile and is a member of Xiphinema simile species complex. The phylogenetic reconstructions inferred from three molecular markers (18S, D2-D3 28S rDNA and cox1) showed that Xiphinema penevi sp. n. is part of Xiphinema pachtaicum-subgroup and is closely related to Xiphinema incertum, Xiphinema pachtaicum, Xiphinema parapachydermum, Xiphinema plesiopachtaicum, Xiphinema astaregiense and Xiphinema pachydermum. Also, a separate “Xiphinema simile-subgroup”, outside the Xiphinema pachtaicum-subgroup and so far consisting only of the parthenogenetic species Xiphinema simile, Xiphinema parasimile, Xiphinema browni sp. n. and probably Xiphinema vallense was formed. New primers for amplification and sequencing of part of the nad4 mitochondrial gene were designed and used.

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Section: Methodsmentioning

confidence: 95%

Morphological and molecular characterisation, and phylogenetic position of X. browni sp. n., X. penevi sp. n. and two known species of Xiphinema americanum-group (Nematoda, Longidoridae)

Lazarova¹,

Peneva²,

Kumari³

2016

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“…Consequently, currently available molecular techniques may help to provide tools for differentiating X. americanum ‐group species that could significantly improve and facilitate the routine identification of these nematodes. Polyphasic identification, based on an integrative strategy of combining molecular techniques with morphology and measurements for species diagnosis, has proved to be an efficient and reliable tool for nematode identification within this and other nematode groups (Derycke et al ., ; Meza, Aballay & Hinrichsen, ; Gutiérrez‐Gutiérrez et al ., , ). In Spain, the X. americanum ‐group was recently studied by Gutiérrez‐Gutiérrez et al .…”

Section: Introductionmentioning

confidence: 99%

“…Consequently, currently available molecular techniques may help to provide tools for differentiating X. americanum-group species that could significantly improve and facilitate the routine identification of these nematodes. Polyphasic identification, based on an integrative strategy of combining molecular techniques with morphology and measurements for species diagnosis, has proved to be an efficient and reliable tool for nematode identification within this and other nematode groups (Derycke et al, 2010;Meza, Aballay & Hinrichsen, 2011;Gutiérrez-Gutiérrez et al, 2012. In Spain, the X. americanum-group was recently studied by Gutiérrez-Gutiérrez et al (2010, 2012 on populations from grapevines and other host-plants in southern Spain, where two new species were described (Xiphinema parapachydermum and Xiphinema paratenuicutis), and four new records for the country were reported (Xiphinema duriense, X. incertum, X. opisthohysterum, Xiphinema santos).…”

Section: Introductionmentioning

confidence: 99%

Cryptic diversity and species delimitation in theXiphinema americanum-group complex (Nematoda: Longidoridae) as inferred from morphometrics and molecular markers

Archidona-Yuste

Navas‐Cortés

Cantalapiedra‐Navarrete

et al. 2016

Zool J Linn Soc

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The Xiphinema americanum-group constitutes a complex of about 55 species of polyphagous plant-ectoparasitic nematodes with a worldwide distribution. This group of plant-parasitic nematodes is one of the most difficult dagger nematode species complexes for diagnosis because the morphology is very conservative and morphometric characters often overlap. We conducted nematode surveys in cultivated and wild olives in southern Spain from 2012 to 2014, from which we identified 16 nematode populations of the X. americanum-group, five of which were tentatively identified as belonging to three new species and are described herein as Xiphinema plesiopachtaicum sp. nov., Xiphinema vallense sp. nov., and Xiphinema astaregiense sp. nov., and 11 populations belonging to nine known species: Xiphinema brevisicum, Xiphinema duriense, Xiphinema incertum, Xiphinema luci, Xiphinema madeirense, Xiphinema opisthohysterum, Xiphinema pachtaicum, Xiphinema parapachydermum, and Xiphinema rivesi. A phenetic study based on multivariate factor analyses was developed to compare some of these related species by using morphometric features. In the factor analysis the first four factors accounted for 73.1% of the total variance of the selected characters, identifying body length, body length/maximum body width (a), body length/ pharyngeal length (b), body length/tail length (c), and tail length/body width at anus (c′) ratios, distance from anterior end to vulva as percentage of body length (V), stylet length, oral aperture-guiding ring distance, and lip region width as key morphometric characters to differentiate a restricted set of species within the X. pachtaicumsubgroup that includes X. plesiopachtaicum sp. nov. and X. vallense sp. nov. Multivariate analysis of variance using these specific characters allowed to differentiate species in the X. pachtaicum complex or groups of them using morphometric characters (body length, a, b, c, c′, V, stylet length, lip region width, oral apertureguiding ring distance, female tail length, and hyaline region length). Phylogenetic analyses based on nuclear ribosomal DNA genes [D2-D3 expansion segments of large ribosomal subunit 28S, and internal transcribed spacer 1 (ITS1)] and the protein-coding mitochondrial gene, cytochrome c oxidase subunit 1 (coxI) were congruent, showing two main clades separating most of the species of X. americanum-subgroup 'sensu stricto' from the X. pachtaicumsubgroup. Agreement between phylogenetic trees and some morphological characters (viz. total stylet length, vulva position, and a ratio) were tested by reconstruction of their histories on rRNA-based trees using parsimony and Bayesian approaches. Thus, integrative taxonomy, based on a combination of multivariate morphological and molecular analyses constitutes a new insight into the identification of X. americanum-group species.

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“…The South African population is both morphometrically and genetically similar to X. peruvianum . Meza et al (2011) indicated that a high homology exists between Chile population of X. peruvianum and X. oxycaudatum identified from Taiwan. The South African population are similar to X. peruvianum but are distinguished by their shorter odontostyle and tail shape.…”

Section: Discussionmentioning

confidence: 94%

First report and molecular characterization of the dagger nematode, Xiphinema oxycaudatum (Nematoda, Dorylaimidae) from South Africa

Daramola¹,

Knoetze²,

Swart³

et al. 2019

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Plant-parasitic nematodes of the genus Xiphinema Cobb, 1913 comprise a complex group of nematode species, some of which are important vectors of plant viruses. During a field survey to determine the soil health of an abandoned honeybush (Cyclopia genistoides) monoculture, a high density of the dagger nematode, Xiphinema oxycaudatum Lamberti & Bleve-Zacheo, 1979 (Nematoda, Dorylaimidae), was observed in soil around the roots of honeybush plants in an abandoned farmland at Bereaville, an old mission station in the Western Cape province of South Africa. Soil samples were taken from the rhizosphere of plants and nematodes were extracted from the soil using a modified extraction tray method. Specimen of the dagger nematodes were processed for scanning electron microscopy, morphological and molecular analysis. Molecular profiling of the nematode species was done in order to give an accurate diagnosis and to effectively discriminate the nematode from other species within the Xiphinema americanum group. Phylogenetic analysis based on the D2D3 expansion segment of the 28S gene supported a close relationship of species within the americanum group, however, the protein-coding cytochrome oxidase (coxI) of the mitochondrial gene provided a useful tool for distinguishing the nematode from other species within the group. This study represents the first report of X. oxycaudatum from South Africa.

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Molecular and morphological characterisation of species within the Xiphinema americanum-group (Dorylaimida: Longidoridae) from the central valley of Chile

Cited by 9 publications

References 6 publications

Morphological and molecular characterisation, and phylogenetic position of X. browni sp. n., X. penevi sp. n. and two known species of Xiphinema americanum-group (Nematoda, Longidoridae)

Morphological and molecular characterisation, and phylogenetic position of X. browni sp. n., X. penevi sp. n. and two known species of Xiphinema americanum-group (Nematoda, Longidoridae)

Cryptic diversity and species delimitation in theXiphinema americanum-group complex (Nematoda: Longidoridae) as inferred from morphometrics and molecular markers

First report and molecular characterization of the dagger nematode, Xiphinema oxycaudatum (Nematoda, Dorylaimidae) from South Africa

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