Molecular and morphological descriptions of Ceratomyxa collarae n. sp. and Ceratomyxa leucosternoni n. sp. from marine ornamental fishes of Indian waters

Sanil, N K; Chandran, Archana; Shamal, P.; Binesh, C. P.

doi:10.1007/s00436-016-5317-6

Cited by 14 publications

(7 citation statements)

References 23 publications

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“…The intraspecific threshold values used for clinid ceratomyxids in present study are higher than commonly reported in other myxozoan clades [ 17 ] but they have been carefully evaluated with regard to the fast evolution of the studied molecular markers in this long-branched parasite group and we recommend using these cut-offs for this specific group of ceratomyxids in future studies. For example, relatively low values of maximum SSU rDNA intraspecific divergences were reported for ceratomyxids from Indian (up to 0.8%) [ 81 ], Australian (up to 1.3%) [ 26 , 27 , 30 ] and North Atlantic fishes (up to 1.6%) [ 46 ], whereas wide ranges up to 7% in C . cottoidii were encountered for South African clinid ceratomyxids (present study).…”

Section: Discussionmentioning

confidence: 99%

Life in a rock pool: Radiation and population genetics of myxozoan parasites in hosts inhabiting restricted spaces

et al. 2018

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IntroductionIntertidal rock pools where fish and invertebrates are in constant close contact due to limited space and water level fluctuations represent ideal conditions to promote life cycles in parasites using these two alternate hosts and to study speciation processes that could contribute to understanding the roles of parasitic species in such ecosystems.Material and methodsGall bladder and liver samples from five clinid fish species (Blenniiformes: Clinidae) were morphologically and molecularly examined to determine the diversity, prevalence, distribution and host specificity of Ceratomyxa parasites (Cnidaria: Myxozoa) in intertidal habitats along the coast of South Africa. Phylogenetic relationships of clinid ceratomyxids based on the SSU rDNA, LSU rDNA and ITS regions were assessed additionally to the investigation of population genetic structure of Ceratomyxa cottoidii and subsequent comparison with the data known from type fish host Clinus cottoides.Results and discussionSeven Ceratomyxa species including previously described Ceratomyxa dehoopi and C. cottoidii were recognized in clinids. They represent a diverse group of rapidly evolving, closely related species with a remarkably high prevalence in their hosts, little host specificity and frequent concurrent infections, most probably as a result of parasite radiation after multiple speciation events triggered by limited host dispersal within restricted spaces. C. cottoidii represents the most common clinid parasite with a population structure characterized by young expanding populations in the south west and south east coast and by older populations in equilibrium on the west coast of its distribution. Parasite and fish host population structures show overlapping patterns and are very likely affected by similar oceanographic barriers possibly due to reduced host dispersal enhancing parasite community differentiation. While fish host specificity had little impact on parasite population structure, the habitat preference of the alternate invertebrate host as well as tidal water exchange may be additional crucial variables affecting the dispersal and associated population structure of C. cottoidii.

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Section: Discussionmentioning

confidence: 99%

Life in a rock pool: Radiation and population genetics of myxozoan parasites in hosts inhabiting restricted spaces

et al. 2018

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“…Molecular characteristics, such as sequence similarity and variable sites, are usually regarded as important evidence for species identification and discrimination of closely related myxosporean species (Liu et al, 2019; Sanil et al, 2017; Zhao et al, 2013). Generalizing, when the similarity of the two sequences is less than 96.4% (Atkinson et al, 2015; Ferguson et al, 2008) or variable sites of intra‐species identification are more than ten (Zhao et al, 2013), it can be considered to be different species.…”

Section: Discussionmentioning

confidence: 99%

“…Traditionally, species identification of myxosporeans was based on myxospore morphology. However, morphological variation of myxospores within a species, or morphological similarities between species create challenges for myxosporean species identification (Ferguson et al, 2008; Liu et al, 2011; Sanil et al, 2017; Thabet et al, 2016; Whipps & Kent, 2006; Zhai et al, 2016). As such, DNA sequence data has become indispensable for investigating the taxonomy and systematics of myxosporeans.…”

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confidence: 99%

Description of Myxidiumpseudocuneiforme n. sp. (Myxosporea: Myxidiidae) from Cyprinuscarpio in China, with the resolution on a taxonomic dilemma of Myxidiumcuneiforme

Chen

Zhang

Whipps

et al. 2021

J Eukaryotic Microbiology

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Investigations on myxozoan parasites of fish from Chongqing in China, revealed two Myxidium cuneiforme‐like myxosporeans infecting the gallbladder of Cyprinus carpio carpio and Carassius auratus. We researched their myxospore morphology, and analyzed their genetic similarity and phylogenic relationships to other myxozoans based on small subunit ribosomal DNA (18S rDNA) sequences. Although both parasites recovered were morphologically similar, the myxosporean isolated from C. auratus was consistent in morphology to Myxidium cuneiforme, which was described from this host species. The parasite isolated from C. c. carpio had overlapping myxospore dimensions to M. cuneiforme, but on average, the polar capsules were not as long. More importantly, this parasite was genetically distinct from M. cuneiforme with 96.3% and 96.5% similarity in two sequences of 18S rDNA, and we propose the name Myxidium pseudocuneiforme n. sp. for this myxozoan from common carp. Its mature myxospores are ellipsoidal and asymmetric with pointed ends in valvular view, arc‐shaped or fusiform in sutural view. The pyriform polar capsules are equal in size, and polar filament with 5–6 coils. This study highlights that molecular characteristics and host specificity are indispensable for myxozoan species identification when presented with the taxonomic dilemma of whether we are observing one species that exhibits slight morphological differences or multiple, but similar, species in different hosts.

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“…This diversity coupled with the plasticity of myxospore morphology of Ceratomyxa causes challenges for species identification. It is thus recommended to incorporate molecular data in species classification [14,15]. Currently, novel species are described using characters that include myxospore morphology, small subunit (SSU) rRNA gene sequences, and information of host species, infection site (tissue tropism), and geographic locality.…”

Section: Introductionmentioning

confidence: 99%

Morphological and genetic analysis of Ceratomyxa saurida Zhao et al. 2015 and Ceratomyxa mai sp. nov. (Myxozoa: Ceratomyxidae) from the East China Sea

Yang

Huang

Bartholomew

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

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We describe Ceratomyxa saurida Zhao et al. 2015 and Ceratomyxa mai sp. nov. (Myxozoa: Ceratomyxidae) from the East China Sea. C. saurida was found in the gallbladders of 3/13 specimens of its type host, Saurida elongata Temminck and Schlegel 1846 (Aulopiformes). Myxospore characters were consistent with the original description to which we have added small subunit (SSU) rRNA gene data. C. mai sp. nov. was found in gallbladders of 3/13 specimens of S. elongata and 5/13 specimens of Neobythites sivicola Jordan and Snyder 1901 (Ophidiiformes). Mature myxospores of C. mai sp. nov. were crescentic in sutural view, with a deeply concave posterior angle 142.2±8.2° (125.8‒158.2°) and an arched anterior side. Shell valves were smooth and equal, 20.9±1.9 (17.3‒24.7) µm thick and 9.2±0.5 (8.1‒9.9) µm long, and joined at a straight, thin sutural plane passing between two nematocysts (polar capsules). The nematocysts were equal-sized, pyriform, 2.6±0.2 (2.4‒2.9) µm long and 2.7±0.2 (2.4‒3.3) µm wide, with their tapered ends pointed toward each other, located in the anterior third of the spore. Sequences of the SSU rRNA gene and internal transcribed spacer 1 showed that the isolates of C. mai sp. nov. obtained from S. elongata and N. sivicola were identical. The SSU rRNA gene sequence of C. mai sp. nov. was distinct from all known myxosporeans and clustered with C. saurida, and then with Ceratomyxa filamentosi Kalatzis, Kokkari and Katharios 2013, both of which also infect Aulopiformes fishes.

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Molecular and morphological descriptions of Ceratomyxa collarae n. sp. and Ceratomyxa leucosternoni n. sp. from marine ornamental fishes of Indian waters

Cited by 14 publications

References 23 publications

Life in a rock pool: Radiation and population genetics of myxozoan parasites in hosts inhabiting restricted spaces

Life in a rock pool: Radiation and population genetics of myxozoan parasites in hosts inhabiting restricted spaces

Description of Myxidiumpseudocuneiforme n. sp. (Myxosporea: Myxidiidae) from Cyprinuscarpio in China, with the resolution on a taxonomic dilemma of Myxidiumcuneiforme

Morphological and genetic analysis of Ceratomyxa saurida Zhao et al. 2015 and Ceratomyxa mai sp. nov. (Myxozoa: Ceratomyxidae) from the East China Sea

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